Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFJ83_RS04020 Genome accession   NZ_CP099382
Coordinates   814169..815149 (+) Length   326 a.a.
NCBI ID   WP_016154397.1    Uniprot ID   R8WNL0
Organism   Citrobacter braakii strain RHB14-SO-C08     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 809169..820149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ83_RS03980 (NFJ83_03980) hemW 809592..810728 (-) 1137 WP_103284209.1 radical SAM family heme chaperone HemW -
  NFJ83_RS03985 (NFJ83_03985) - 810721..811314 (-) 594 WP_016154402.1 XTP/dITP diphosphatase -
  NFJ83_RS03990 (NFJ83_03990) yggU 811322..811612 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  NFJ83_RS03995 (NFJ83_03995) - 811609..812175 (-) 567 WP_016154401.1 YggT family protein -
  NFJ83_RS04000 (NFJ83_04000) - 812194..812898 (-) 705 WP_016154400.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFJ83_RS04005 (NFJ83_04005) - 812871..813041 (+) 171 Protein_786 type IV pili twitching motility protein PilT -
  NFJ83_RS04010 (NFJ83_04010) - 813556..813975 (+) 420 WP_016157440.1 DUF3828 domain-containing protein -
  NFJ83_RS04015 (NFJ83_04015) - 813981..814151 (-) 171 Protein_788 YggS family pyridoxal phosphate enzyme -
  NFJ83_RS04020 (NFJ83_04020) pilT 814169..815149 (+) 981 WP_016154397.1 type IV pilus twitching motility protein PilT Machinery gene
  NFJ83_RS04025 (NFJ83_04025) ruvX 815146..815562 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  NFJ83_RS04030 (NFJ83_04030) - 815562..816125 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  NFJ83_RS04035 (NFJ83_04035) gshB 816302..817249 (-) 948 WP_016154394.1 glutathione synthase -
  NFJ83_RS04040 (NFJ83_04040) rsmE 817262..817993 (-) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFJ83_RS04045 (NFJ83_04045) endA 818069..818776 (-) 708 WP_103284208.1 deoxyribonuclease I -
  NFJ83_RS04050 (NFJ83_04050) - 818871..819368 (-) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36010.12 Da        Isoelectric Point: 6.3392

>NTDB_id=699241 NFJ83_RS04020 WP_016154397.1 814169..815149(+) (pilT) [Citrobacter braakii strain RHB14-SO-C08]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTPPDVENLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=699241 NFJ83_RS04020 WP_016154397.1 814169..815149(+) (pilT) [Citrobacter braakii strain RHB14-SO-C08]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCGGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGCACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACTCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCATATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCACAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTTGAACTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8WNL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365