Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFJ84_RS03825 Genome accession   NZ_CP099381
Coordinates   791854..792834 (+) Length   326 a.a.
NCBI ID   WP_279265517.1    Uniprot ID   -
Organism   Citrobacter braakii strain RHB16-E2-C02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 786854..797834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ84_RS03795 (NFJ84_03790) - 787230..788216 (-) 987 WP_169329839.1 TRAP transporter substrate-binding protein -
  NFJ84_RS03800 (NFJ84_03795) hemW 788530..789666 (-) 1137 WP_279265516.1 radical SAM family heme chaperone HemW -
  NFJ84_RS03805 (NFJ84_03800) - 789659..790252 (-) 594 WP_019077664.1 XTP/dITP diphosphatase -
  NFJ84_RS03810 (NFJ84_03805) yggU 790260..790550 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  NFJ84_RS03815 (NFJ84_03810) - 790547..791113 (-) 567 WP_016154401.1 YggT family protein -
  NFJ84_RS03820 (NFJ84_03815) - 791132..791836 (-) 705 WP_016154400.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFJ84_RS03825 (NFJ84_03820) pilT 791854..792834 (+) 981 WP_279265517.1 type IV pilus twitching motility protein PilT Machinery gene
  NFJ84_RS03830 (NFJ84_03825) ruvX 792831..793247 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  NFJ84_RS03835 (NFJ84_03830) - 793247..793810 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  NFJ84_RS03840 (NFJ84_03835) gshB 793987..794934 (-) 948 WP_016154394.1 glutathione synthase -
  NFJ84_RS03845 (NFJ84_03840) rsmE 794947..795678 (-) 732 WP_279265518.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFJ84_RS03850 (NFJ84_03845) endA 795754..796461 (-) 708 WP_279265519.1 deoxyribonuclease I -
  NFJ84_RS03855 (NFJ84_03850) - 796556..797053 (-) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35911.97 Da        Isoelectric Point: 5.9920

>NTDB_id=699218 NFJ84_RS03825 WP_279265517.1 791854..792834(+) (pilT) [Citrobacter braakii strain RHB16-E2-C02]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIGGKVEIAPFTPPDVEDLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=699218 NFJ84_RS03825 WP_279265517.1 791854..792834(+) (pilT) [Citrobacter braakii strain RHB16-E2-C02]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATAGGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGGATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTCCA
GACCCCCGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCACATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCGTCAACACGCCCGCCGTGGGTAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Neisseria meningitidis 8013

46.037

100

0.463
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Neisseria gonorrhoeae MS11

45.732

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365