Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFK10_RS03730 Genome accession   NZ_CP099378
Coordinates   758175..759155 (+) Length   326 a.a.
NCBI ID   WP_016154397.1    Uniprot ID   R8WNL0
Organism   Citrobacter braakii strain RHB21-E1-C04     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 753175..764155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFK10_RS03685 (NFK10_03680) hemW 753403..754539 (-) 1137 WP_016157443.1 radical SAM family heme chaperone HemW -
  NFK10_RS03690 (NFK10_03685) - 754532..755125 (-) 594 WP_016154402.1 XTP/dITP diphosphatase -
  NFK10_RS03695 (NFK10_03690) yggU 755133..755423 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  NFK10_RS03700 (NFK10_03695) - 755420..755986 (-) 567 WP_016154401.1 YggT family protein -
  NFK10_RS03705 (NFK10_03700) - 756005..756709 (-) 705 WP_016154400.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFK10_RS03710 (NFK10_03705) - 756682..756852 (+) 171 Protein_727 type IV pili twitching motility protein PilT -
  NFK10_RS03715 (NFK10_03710) - 757203..757562 (+) 360 WP_016157441.1 hypothetical protein -
  NFK10_RS03720 (NFK10_03715) - 757562..757981 (+) 420 WP_016157440.1 DUF3828 domain-containing protein -
  NFK10_RS03725 (NFK10_03720) - 757987..758157 (-) 171 Protein_730 YggS family pyridoxal phosphate enzyme -
  NFK10_RS03730 (NFK10_03725) pilT 758175..759155 (+) 981 WP_016154397.1 type IV pilus twitching motility protein PilT Machinery gene
  NFK10_RS03735 (NFK10_03730) ruvX 759152..759568 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  NFK10_RS03740 (NFK10_03735) - 759568..760131 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  NFK10_RS03745 (NFK10_03740) gshB 760308..761255 (-) 948 WP_016154394.1 glutathione synthase -
  NFK10_RS03750 (NFK10_03745) rsmE 761268..761999 (-) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFK10_RS03755 (NFK10_03750) endA 762075..762782 (-) 708 WP_016157439.1 deoxyribonuclease I -
  NFK10_RS03760 (NFK10_03755) - 762877..763374 (-) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36010.12 Da        Isoelectric Point: 6.3392

>NTDB_id=699172 NFK10_RS03730 WP_016154397.1 758175..759155(+) (pilT) [Citrobacter braakii strain RHB21-E1-C04]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTPPDVENLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=699172 NFK10_RS03730 WP_016154397.1 758175..759155(+) (pilT) [Citrobacter braakii strain RHB21-E1-C04]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCGGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGCACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACTCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCATATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCACAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTTGAACTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8WNL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365