Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFK58_RS04660 Genome accession   NZ_CP099373
Coordinates   939459..940439 (+) Length   326 a.a.
NCBI ID   WP_275396656.1    Uniprot ID   -
Organism   Citrobacter portucalensis strain RHB38-SO-C05     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 934459..945439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFK58_RS04630 (NFK58_04660) ansB 934972..936018 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  NFK58_RS04635 (NFK58_04665) hemW 936135..937271 (-) 1137 WP_123267928.1 radical SAM family heme chaperone HemW -
  NFK58_RS04640 (NFK58_04670) - 937264..937857 (-) 594 WP_123267929.1 XTP/dITP diphosphatase -
  NFK58_RS04645 (NFK58_04675) yggU 937865..938155 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  NFK58_RS04650 (NFK58_04680) - 938152..938718 (-) 567 WP_003825417.1 YggT family protein -
  NFK58_RS04655 (NFK58_04685) - 938737..939441 (-) 705 WP_057067562.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFK58_RS04660 (NFK58_04690) pilT 939459..940439 (+) 981 WP_275396656.1 type IV pilus twitching motility protein PilT Machinery gene
  NFK58_RS04665 (NFK58_04695) ruvX 940436..940852 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  NFK58_RS04670 (NFK58_04700) - 940852..941415 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  NFK58_RS04675 (NFK58_04705) gshB 941592..942539 (-) 948 WP_003027083.1 glutathione synthase -
  NFK58_RS04680 (NFK58_04710) rsmE 942559..943290 (-) 732 WP_008785768.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFK58_RS04685 (NFK58_04715) endA 943365..944072 (-) 708 WP_008785767.1 deoxyribonuclease I -
  NFK58_RS04690 (NFK58_04720) - 944167..944664 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35863.97 Da        Isoelectric Point: 6.4093

>NTDB_id=699060 NFK58_RS04660 WP_275396656.1 939459..940439(+) (pilT) [Citrobacter portucalensis strain RHB38-SO-C05]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIHGKVDIAPFTTPDVEKLLMCWLSEQQQVQWREQGQVDFAIALAGSRRLRA
SAFAHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLSSENGLILVTGATGSGKSTTLAAMVEYLNQQVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=699060 NFK58_RS04660 WP_275396656.1 939459..940439(+) (pilT) [Citrobacter portucalensis strain RHB38-SO-C05]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACATGGAAAAGTCGACATCGCACCATTTACTACGCCTGACGTGGAGAAGCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGGTAGATTTTGCTATTGCGCTGGCAGGTTCCCGGCGGCTGCGCGCC
AGTGCTTTTGCTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTCCCGCTTGATTGCCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGAGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTTGAGTACCTTAATCAGCAAGTTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAACGGGAGGTGGGCGTGCACTGCGCTTCTTTTGCCGCGGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGATGTGGAAACCATTCGTCTGGCGTTAACCG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCGCAGGCTATCGCACGGCTGGTGGATACC
TTTCCCGCACAGGAAAAAGATCCGGTGCGTAACCAACTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAGGA
AGATAAACAGGGAGGGCGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGCCAGCAGCAGCGT
CAGGCGCAGGGGCGGATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

46.667

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.364

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Acinetobacter baumannii D1279779

45.593

100

0.46
  pilT Acinetobacter baumannii strain A118

45.593

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.289

100

0.457
  pilT Acinetobacter baylyi ADP1

45.289

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365