Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFK63_RS03915 Genome accession   NZ_CP099367
Coordinates   788923..789903 (+) Length   326 a.a.
NCBI ID   WP_279275496.1    Uniprot ID   -
Organism   Citrobacter portucalensis strain RHB40-E3-C05     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 783923..794903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFK63_RS03885 (NFK63_03885) ansB 784438..785484 (+) 1047 WP_126317426.1 L-asparaginase 2 -
  NFK63_RS03890 (NFK63_03890) hemW 785599..786735 (-) 1137 WP_126317427.1 radical SAM family heme chaperone HemW -
  NFK63_RS03895 (NFK63_03895) - 786728..787321 (-) 594 WP_003825414.1 XTP/dITP diphosphatase -
  NFK63_RS03900 (NFK63_03900) yggU 787329..787619 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  NFK63_RS03905 (NFK63_03905) - 787616..788182 (-) 567 WP_003825417.1 YggT family protein -
  NFK63_RS03910 (NFK63_03910) - 788201..788905 (-) 705 WP_057067562.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFK63_RS03915 (NFK63_03915) pilT 788923..789903 (+) 981 WP_279275496.1 type IV pilus twitching motility protein PilT Machinery gene
  NFK63_RS03920 (NFK63_03920) ruvX 789900..790316 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  NFK63_RS03925 (NFK63_03925) - 790316..790879 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  NFK63_RS03930 (NFK63_03930) gshB 791055..792002 (-) 948 WP_003027083.1 glutathione synthase -
  NFK63_RS03935 (NFK63_03935) rsmE 792022..792753 (-) 732 WP_003825432.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFK63_RS03940 (NFK63_03940) endA 792828..793535 (-) 708 WP_008785767.1 deoxyribonuclease I -
  NFK63_RS03945 (NFK63_03945) - 793630..794127 (-) 498 WP_279275497.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35940.02 Da        Isoelectric Point: 6.3379

>NTDB_id=699013 NFK63_RS03915 WP_279275496.1 788923..789903(+) (pilT) [Citrobacter portucalensis strain RHB40-E3-C05]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVEYLLMCWLSEQQQVQWQEQGQLDFAIALAGSQRLRA
SAFVHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQHVAGHILTLEDPIE
YRYTSQRCLIHQREVGVHCASFAAGLRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FSATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=699013 NFK63_RS03915 WP_279275496.1 788923..789903(+) (pilT) [Citrobacter portucalensis strain RHB40-E3-C05]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGTATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCAGGAACAGGGGCAGCTGGATTTTGCTATTGCGCTGGCAGGTTCCCAGCGGCTGCGCGCC
AGTGCTTTTGTTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTCCCGCTTGATTGTCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTAGAGTACCTTAATCAGCATGTTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCACCAACGGGAGGTGGGCGTACACTGCGCTTCTTTTGCCGCCGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGATGTCATTTTGCTGGGCGAACTGCGCGACGCGGAAACCATTCGTCTGGCGTTAACGG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCACAGGCCATCGCGCGGCTGGTGGACACC
TTTTCGGCAACAGAGAAGGATCCGGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAACAGGGAGGGCGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCTGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGCCAGCAGCAGCGT
CAGGCGCAGGGGCGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

46.061

100

0.466
  pilT Neisseria gonorrhoeae MS11

45.758

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

45.593

100

0.46
  pilT Acinetobacter baylyi ADP1

45.289

100

0.457
  pilT Acinetobacter baumannii D1279779

45.289

100

0.457
  pilT Acinetobacter baumannii strain A118

45.289

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter nosocomialis M2

44.985

100

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371