Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFK67_RS19505 Genome accession   NZ_CP099366
Coordinates   4056076..4057056 (-) Length   326 a.a.
NCBI ID   WP_155574361.1    Uniprot ID   -
Organism   Citrobacter braakii strain RHB41-E2-C02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4051076..4062056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFK67_RS19475 (NFK67_19465) - 4051857..4052354 (+) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -
  NFK67_RS19480 (NFK67_19470) endA 4052449..4053156 (+) 708 WP_019077649.1 deoxyribonuclease I -
  NFK67_RS19485 (NFK67_19475) rsmE 4053232..4053963 (+) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFK67_RS19490 (NFK67_19480) gshB 4053976..4054923 (+) 948 WP_016154394.1 glutathione synthase -
  NFK67_RS19495 (NFK67_19485) - 4055100..4055663 (+) 564 WP_016154395.1 YqgE/AlgH family protein -
  NFK67_RS19500 (NFK67_19490) ruvX 4055663..4056079 (+) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  NFK67_RS19505 (NFK67_19495) pilT 4056076..4057056 (-) 981 WP_155574361.1 type IV pilus twitching motility protein PilT Machinery gene
  NFK67_RS19510 (NFK67_19500) - 4057074..4057244 (+) 171 Protein_3822 YggS family pyridoxal phosphate enzyme -
  NFK67_RS19515 (NFK67_19505) - 4057250..4057669 (-) 420 WP_279267271.1 DUF3828 domain-containing protein -
  NFK67_RS19520 (NFK67_19510) - 4058193..4058357 (-) 165 Protein_3824 type IV pili twitching motility protein PilT -
  NFK67_RS19525 (NFK67_19515) - 4058330..4059034 (+) 705 WP_016154400.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFK67_RS19530 (NFK67_19520) - 4059053..4059619 (+) 567 WP_016154401.1 YggT family protein -
  NFK67_RS19535 (NFK67_19525) yggU 4059616..4059906 (+) 291 WP_005123286.1 DUF167 family protein YggU -
  NFK67_RS19540 (NFK67_19530) - 4059914..4060507 (+) 594 WP_016154402.1 XTP/dITP diphosphatase -
  NFK67_RS19545 (NFK67_19535) hemW 4060500..4061636 (+) 1137 WP_279267272.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35938.01 Da        Isoelectric Point: 6.1727

>NTDB_id=699004 NFK67_RS19505 WP_155574361.1 4056076..4057056(-) (pilT) [Citrobacter braakii strain RHB41-E2-C02]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIGGKVEIAPFTPPDVENLLMCWLNEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=699004 NFK67_RS19505 WP_155574361.1 4056076..4057056(-) (pilT) [Citrobacter braakii strain RHB41-E2-C02]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATAGGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAATG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACCCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCACATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGACTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGTTGGAGGA
AGATAAGCAGGGAGGACGCGTTGCGCTATTCGAACTGCTCGTCAACACTCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Legionella pneumophila strain Lp02

47.799

97.546

0.466
  pilT Legionella pneumophila strain ERS1305867

47.799

97.546

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.587

97.239

0.414
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

40.323

95.092

0.383
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365