Detailed information NTG0698968

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: NFL11_RS04095
• Genome accession: NZ_CP099364
• Coordinates: 817208..818188 (+)
• Length: 326 a.a.
• NCBI ID: WP_103928580.1
• Uniprot ID: -
• Organism: Citrobacter braakii strain RHB44-SE-C08
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	816193..816890	817208..818188	flank	318

Gene organization within MGE regions

Location: 816193..818188

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NFL11_RS04085 (NFL11_04085)	-	816193..816890 (+)	698	WP_223200906.1	IS1-like element IS1A family transposase	-
NFL11_RS04090 (NFL11_04090)	-	817041..817190 (-)	150	Protein_803	YggS family pyridoxal phosphate enzyme	-
NFL11_RS04095 (NFL11_04095)	pilT	817208..818188 (+)	981	WP_103928580.1	type IV pilus twitching motility protein PilT	Machinery gene

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35880.90 Da
• Isoelectric Point: 6.1727

>NTDB_id=698968 NFL11_RS04095 WP_103928580.1 817208..818188(+) (pilT) [Citrobacter braakii strain RHB44-SE-C08]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIGGKVEIAPFTPPDVENLLTCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide

• Length: 981 bp

>NTDB_id=698968 NFL11_RS04095 WP_103928580.1 817208..818188(+) (pilT) [Citrobacter braakii strain RHB44-SE-C08]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATAGGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGACGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACCCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCATATTCTAACTCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGACTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGTTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCGTCAACACTCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (56-265)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	50.765	100	0.509
pilT	Vibrio cholerae strain A1552	50.765	100	0.509
pilT	Neisseria meningitidis 8013	46.646	100	0.469
pilT	Neisseria gonorrhoeae MS11	46.341	100	0.466
pilT	Legionella pneumophila strain ERS1305867	47.484	97.546	0.463
pilT	Legionella pneumophila strain Lp02	47.484	97.546	0.463
pilT	Acinetobacter baylyi ADP1	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	45.872	100	0.46
pilT	Pseudomonas stutzeri DSM 10701	45.872	100	0.46
pilT	Acinetobacter baumannii D1279779	45.872	100	0.46
pilT	Pseudomonas aeruginosa PAK	45.566	100	0.457
pilT	Acinetobacter nosocomialis M2	45.566	100	0.457
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.271	97.239	0.411
pilU	Pseudomonas stutzeri DSM 10701	38.788	100	0.393
pilU	Vibrio cholerae strain A1552	39.13	98.773	0.386
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362