Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFJ86_RS09475 Genome accession   NZ_CP099349
Coordinates   1922290..1923270 (-) Length   326 a.a.
NCBI ID   WP_181202821.1    Uniprot ID   A0A7W3ETT0
Organism   Escherichia fergusonii strain RHB16-E2-C07     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1917290..1928270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ86_RS09445 (NFJ86_09420) - 1918014..1918511 (+) 498 WP_104917519.1 SprT family zinc-dependent metalloprotease -
  NFJ86_RS09450 (NFJ86_09425) endA 1918606..1919313 (+) 708 WP_000286513.1 deoxyribonuclease I -
  NFJ86_RS09455 (NFJ86_09430) rsmE 1919393..1920124 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFJ86_RS09460 (NFJ86_09435) gshB 1920137..1921087 (+) 951 WP_000593298.1 glutathione synthase -
  NFJ86_RS09465 (NFJ86_09440) - 1921196..1921759 (+) 564 WP_002431379.1 YqgE/AlgH family protein -
  NFJ86_RS09470 (NFJ86_09445) ruvX 1921759..1922175 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  NFJ86_RS09475 (NFJ86_09450) pilT 1922290..1923270 (-) 981 WP_181202821.1 type IV pilus twitching motility protein PilT Machinery gene
  NFJ86_RS09480 (NFJ86_09455) yggS 1923288..1923992 (+) 705 WP_001723871.1 pyridoxal phosphate homeostasis protein -
  NFJ86_RS09485 (NFJ86_09460) yggT 1924010..1924576 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NFJ86_RS09490 (NFJ86_09465) yggU 1924573..1924863 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  NFJ86_RS09495 (NFJ86_09470) - 1924871..1925464 (+) 594 WP_001174747.1 XTP/dITP diphosphatase -
  NFJ86_RS09500 (NFJ86_09475) hemW 1925457..1926593 (+) 1137 WP_181202822.1 radical SAM family heme chaperone HemW -
  NFJ86_RS09505 (NFJ86_09480) - 1926907..1927893 (+) 987 WP_181668776.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35941.18 Da        Isoelectric Point: 5.7980

>NTDB_id=698908 NFJ86_RS09475 WP_181202821.1 1922290..1923270(-) (pilT) [Escherichia fergusonii strain RHB16-E2-C07]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQLR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698908 NFJ86_RS09475 WP_181202821.1 1922290..1923270(-) (pilT) [Escherichia fergusonii strain RHB16-E2-C07]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATGCGCCGGACGTGGAAGAGCTATTGCGGGAGTGGCTGGATG
ACGACCAGCGGGCAATATTGCTGGAGAATGGTCAGTTAGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTAACGGGGGCGACGGGAAGTGGAAAAT
CTACCACGCTGGCAGCGATGGTTGGTTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGGTGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGACATCTGGTGCTGGCGACCTTACATACACGCGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCCGGGAGCTTGCGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGAAGAGTAGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGAGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCTGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7W3ETT0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.061

100

0.402
  pilU Pseudomonas stutzeri DSM 10701

38.344

100

0.383
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368