Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFL02_RS17275 Genome accession   NZ_CP099328
Coordinates   3589804..3590784 (-) Length   326 a.a.
NCBI ID   WP_071986121.1    Uniprot ID   -
Organism   Escherichia fergusonii strain RHB44-C21     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3584804..3595784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFL02_RS17245 (NFL02_17220) - 3585636..3586133 (+) 498 WP_001296359.1 SprT family zinc-dependent metalloprotease -
  NFL02_RS17250 (NFL02_17225) endA 3586228..3586935 (+) 708 WP_000286513.1 deoxyribonuclease I -
  NFL02_RS17255 (NFL02_17230) rsmE 3587015..3587746 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFL02_RS17260 (NFL02_17235) gshB 3587759..3588709 (+) 951 WP_000593298.1 glutathione synthase -
  NFL02_RS17265 (NFL02_17240) - 3588818..3589381 (+) 564 WP_002431379.1 YqgE/AlgH family protein -
  NFL02_RS17270 (NFL02_17245) ruvX 3589381..3589797 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  NFL02_RS17275 (NFL02_17250) pilT 3589804..3590784 (-) 981 WP_071986121.1 type IV pilus twitching motility protein PilT Machinery gene
  NFL02_RS17280 (NFL02_17255) yggS 3590802..3591506 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  NFL02_RS17285 (NFL02_17260) yggT 3591524..3592090 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NFL02_RS17290 (NFL02_17265) yggU 3592087..3592377 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  NFL02_RS17295 (NFL02_17270) - 3592385..3592978 (+) 594 WP_182258112.1 XTP/dITP diphosphatase -
  NFL02_RS17300 (NFL02_17275) hemW 3592971..3594107 (+) 1137 WP_182258113.1 radical SAM family heme chaperone HemW -
  NFL02_RS17305 (NFL02_17280) - 3594182..3595186 (-) 1005 WP_228932164.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35982.18 Da        Isoelectric Point: 5.6101

>NTDB_id=698829 NFL02_RS17275 WP_071986121.1 3589804..3590784(-) (pilT) [Escherichia fergusonii strain RHB44-C21]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSYCPQLEQLGAPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698829 NFL02_RS17275 WP_071986121.1 3589804..3590784(-) (pilT) [Escherichia fergusonii strain RHB44-C21]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTGGAAGAGCTATTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGTTAGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGTTGTTACCTTCGTATTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGAAGTGGAAAAT
CTACCACGCTGGCAGCGATGGTTGGTTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGGTGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGCATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACCG
CGGCAGAAACCGGACATCTGGTGCTGGCAACCTTGCATACGCGTGGTGCCGCGCAGGCGGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCCGGGAGCTTGCGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATACGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACTGGGCAGCAGGTGGGGATGTTGACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368