Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFK08_RS18855 Genome accession   NZ_CP099308
Coordinates   3929186..3930166 (-) Length   326 a.a.
NCBI ID   WP_248198634.1    Uniprot ID   -
Organism   Enterobacter roggenkampii strain RHB21-E1-C01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3924186..3935166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFK08_RS18825 (NFK08_18815) - 3925067..3925564 (+) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -
  NFK08_RS18830 (NFK08_18820) endA 3925659..3926366 (+) 708 WP_021242074.1 deoxyribonuclease I -
  NFK08_RS18835 (NFK08_18825) rsmE 3926418..3927149 (+) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFK08_RS18840 (NFK08_18830) gshB 3927169..3928116 (+) 948 WP_008499751.1 glutathione synthase -
  NFK08_RS18845 (NFK08_18835) - 3928199..3928759 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  NFK08_RS18850 (NFK08_18840) ruvX 3928759..3929175 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  NFK08_RS18855 (NFK08_18845) pilT 3929186..3930166 (-) 981 WP_248198634.1 type IV pilus twitching motility protein PilT Machinery gene
  NFK08_RS18860 (NFK08_18850) - 3930184..3930885 (+) 702 WP_008499756.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFK08_RS18865 (NFK08_18855) - 3930907..3931473 (+) 567 WP_008499757.1 YggT family protein -
  NFK08_RS18870 (NFK08_18860) yggU 3931470..3931766 (+) 297 WP_008499758.1 DUF167 family protein YggU -
  NFK08_RS18875 (NFK08_18865) - 3931770..3932363 (+) 594 WP_032664352.1 XTP/dITP diphosphatase -
  NFK08_RS18880 (NFK08_18870) hemW 3932356..3933504 (+) 1149 WP_023294525.1 radical SAM family heme chaperone HemW -
  NFK08_RS18885 (NFK08_18875) - 3933689..3934405 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  NFK08_RS18890 (NFK08_18880) - 3934462..3934788 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35413.45 Da        Isoelectric Point: 7.1630

>NTDB_id=698602 NFK08_RS18855 WP_248198634.1 3929186..3930166(-) (pilT) [Enterobacter roggenkampii strain RHB21-E1-C01]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPSPDLGALLKAWLNDEQQGIWWANGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRVIPELLTNASGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSARCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLVPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGMIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698602 NFK08_RS18855 WP_248198634.1 3929186..3930166(-) (pilT) [Enterobacter roggenkampii strain RHB21-E1-C01]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCTCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTTCTCCGGACCTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAACGGGCAGGTGGATTTTGCCGCGACGGTGGCGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGAGTCTCTGTAACGCTACGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCGCTGGG
CGCGCCGAGGGTTATCCCGGAACTGTTAACCAATGCGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATTCTCACGCTCGAAGATCCGGTGGAG
TTTATCTATCAGAGCGCACGTTGCCTGATCCAGCAGCGGGAGATCGGCCAGCACAGCCCGTCATTTGCCGATGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTGATTTTACTGGGAGAGTTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAAGCTGGTTCC
TGACTTACAGGGCGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACCGCGGCGGCGGCGAATCTGATACGAGAAGGAA
AAACGTGGCAGCTGCCCGGCATGATTCAAACCGGACAGCAGGCAGGCATGCAGAACTTTGACCAAAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.695

100

0.5
  pilT Vibrio cholerae strain A1552

49.695

100

0.5
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

40.483

100

0.411
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365