Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFK05_RS03835 Genome accession   NZ_CP099306
Coordinates   798113..799093 (+) Length   326 a.a.
NCBI ID   WP_014839535.1    Uniprot ID   A0A249WJI3
Organism   Klebsiella michiganensis strain RHB20-SO-C01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 793113..804093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFK05_RS03805 (NFK05_03805) - 793495..794481 (-) 987 WP_049071806.1 TRAP transporter substrate-binding protein -
  NFK05_RS03810 (NFK05_03810) hemW 794786..795922 (-) 1137 WP_032751248.1 radical SAM family heme chaperone HemW -
  NFK05_RS03815 (NFK05_03815) - 795915..796508 (-) 594 WP_004854157.1 XTP/dITP diphosphatase -
  NFK05_RS03820 (NFK05_03820) yggU 796521..796811 (-) 291 WP_004854155.1 DUF167 family protein YggU -
  NFK05_RS03825 (NFK05_03825) - 796808..797374 (-) 567 WP_181251419.1 YggT family protein -
  NFK05_RS03830 (NFK05_03830) - 797392..798096 (-) 705 WP_025105939.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFK05_RS03835 (NFK05_03835) pilT 798113..799093 (+) 981 WP_014839535.1 type IV pilus twitching motility protein PilT Machinery gene
  NFK05_RS03840 (NFK05_03840) - 799442..800095 (+) 654 WP_014226837.1 LuxR C-terminal-related transcriptional regulator -
  NFK05_RS03845 (NFK05_03845) ruvX 800151..800567 (-) 417 WP_014226836.1 Holliday junction resolvase RuvX -
  NFK05_RS03850 (NFK05_03850) - 800567..801130 (-) 564 WP_014226835.1 YqgE/AlgH family protein -
  NFK05_RS03855 (NFK05_03855) gshB 801307..802254 (-) 948 WP_014226834.1 glutathione synthase -
  NFK05_RS03860 (NFK05_03860) rsmE 802275..803006 (-) 732 WP_032720268.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFK05_RS03865 (NFK05_03865) endA 803098..803805 (-) 708 WP_014226832.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35210.42 Da        Isoelectric Point: 6.7582

>NTDB_id=698567 NFK05_RS03835 WP_014839535.1 798113..799093(+) (pilT) [Klebsiella michiganensis strain RHB20-SO-C01]
MELEEIVALSVKHNVSDLHLCNASTPRWRRQGKLEPAPFTSPDIGKILADWLNAEQLAQWQACGQVDFALAPAGGPRLRA
SAFVHNRGLSLALRLLAETCPQLADLGAPAALTELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLNGHILTLEDPVE
FIHRSERCLIQQREIGRHCPSFAAALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRSQLAGSLCAVLAQKLFPDTAGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQFGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698567 NFK05_RS03835 WP_014839535.1 798113..799093(+) (pilT) [Klebsiella michiganensis strain RHB20-SO-C01]
ATGGAACTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCTTCAACACCGCG
CTGGCGTCGCCAGGGCAAGCTTGAGCCCGCGCCGTTTACCTCTCCGGATATTGGCAAGATACTCGCTGATTGGCTCAACG
CCGAGCAGCTCGCCCAGTGGCAGGCCTGCGGACAGGTCGATTTCGCGCTGGCTCCGGCCGGCGGACCGCGCCTGCGCGCC
AGCGCATTTGTCCACAACCGCGGTCTGTCGCTGGCCCTGCGTCTGCTGGCGGAAACCTGTCCGCAGCTGGCGGATCTCGG
CGCGCCGGCCGCCTTAACGGAGCTGCTCAATGAGGAGAGCGGGCTTATCCTGGTCACGGGCGCCACCGGTAGCGGGAAAT
CCACCACCCTGGCGGCGATGGTGGGCTATCTTAATCAGCGCCTCAATGGCCATATCCTGACGCTGGAAGATCCGGTTGAG
TTTATTCATCGTAGCGAGCGCTGCCTGATCCAGCAGCGGGAAATCGGTCGCCACTGCCCCTCATTTGCCGCGGCCCTGCG
CGCTGCGCTGCGTCAGGATCCGGATGTCATTCTGCTAGGGGAACTGCGTGACAGCGAAACGATCCGCCTGGCATTGACCG
CGGCGGAGACCGGACATCTGGTTATGGCGACGCTGCATACGCGCGGCGCGGCACAGGCGGTTGAAAGGCTGGTGGATGTT
TTTCCTGCCCAGGAGAAAGAGCAGGTTCGTAGCCAGCTGGCGGGAAGTTTATGCGCGGTGCTGGCACAAAAATTGTTTCC
GGATACTGCTGGCGGAAGAGTTGCGCTATACGAGCTTTTGGTTAATACCCCGGCGGTGGCGAATTTGATCCGCGAGGGGA
AAACGCATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAATTCGGGATGCAAACTTTTACGCAAAGCTTTCAGCAACGT
ACGGCTGCGGGTCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249WJI3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.848

100

0.503
  pilT Vibrio cholerae strain A1552

49.848

100

0.503
  pilT Neisseria meningitidis 8013

48.182

100

0.488
  pilT Neisseria gonorrhoeae MS11

47.879

100

0.485
  pilT Legionella pneumophila strain Lp02

48.113

97.546

0.469
  pilT Legionella pneumophila strain ERS1305867

48.113

97.546

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.201

100

0.466
  pilT Acinetobacter baumannii strain A118

45.455

100

0.46
  pilT Acinetobacter baumannii D1279779

45.455

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.152

100

0.457
  pilT Acinetobacter baylyi ADP1

44.985

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.414
  pilU Vibrio cholerae strain A1552

40.683

98.773

0.402
  pilU Acinetobacter baylyi ADP1

38.39

99.08

0.38