Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFJ16_RS03640 Genome accession   NZ_CP099303
Coordinates   748523..749503 (+) Length   326 a.a.
NCBI ID   WP_016150970.1    Uniprot ID   A0AAW7G9A5
Organism   Citrobacter freundii strain RHB02-E1-C01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 743523..754503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ16_RS03610 (NFJ16_03600) ansB 744037..745083 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  NFJ16_RS03615 (NFJ16_03605) hemW 745199..746335 (-) 1137 WP_032937402.1 radical SAM family heme chaperone HemW -
  NFJ16_RS03620 (NFJ16_03610) - 746328..746921 (-) 594 WP_003027104.1 XTP/dITP diphosphatase -
  NFJ16_RS03625 (NFJ16_03615) yggU 746929..747219 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  NFJ16_RS03630 (NFJ16_03620) - 747216..747782 (-) 567 WP_048217172.1 YggT family protein -
  NFJ16_RS03635 (NFJ16_03625) - 747801..748505 (-) 705 WP_048217173.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFJ16_RS03640 (NFJ16_03630) pilT 748523..749503 (+) 981 WP_016150970.1 type IV pilus twitching motility protein PilT Machinery gene
  NFJ16_RS03645 (NFJ16_03635) ruvX 749500..749916 (-) 417 WP_003027087.1 Holliday junction resolvase RuvX -
  NFJ16_RS03650 (NFJ16_03640) - 749916..750479 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  NFJ16_RS03655 (NFJ16_03645) gshB 750591..751538 (-) 948 WP_003027083.1 glutathione synthase -
  NFJ16_RS03660 (NFJ16_03650) rsmE 751558..752289 (-) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFJ16_RS03665 (NFJ16_03655) endA 752364..753071 (-) 708 WP_016150969.1 deoxyribonuclease I -
  NFJ16_RS03670 (NFJ16_03660) - 753166..753663 (-) 498 WP_279264347.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35926.98 Da        Isoelectric Point: 6.4255

>NTDB_id=698521 NFJ16_RS03640 WP_016150970.1 748523..749503(+) (pilT) [Citrobacter freundii strain RHB02-E1-C01]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRRGKVESAPFTTPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRA
SAFVHQQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGAHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698521 NFJ16_RS03640 WP_016150970.1 748523..749503(+) (pilT) [Citrobacter freundii strain RHB02-E1-C01]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCCGCGCG
TTGGCGCAGGCGTGGAAAAGTCGAAAGCGCACCGTTTACCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCAGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAATGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCGCCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAAACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTTGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTACTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTTTACAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365