Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFJ20_RS03775 Genome accession   NZ_CP099298
Coordinates   780903..781883 (+) Length   326 a.a.
NCBI ID   WP_279274269.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHB02-E3-C01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 775903..786883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ20_RS03745 (NFJ20_03740) ansB 776417..777463 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  NFJ20_RS03750 (NFJ20_03745) hemW 777579..778715 (-) 1137 WP_279274268.1 radical SAM family heme chaperone HemW -
  NFJ20_RS03755 (NFJ20_03750) - 778708..779301 (-) 594 WP_003027104.1 XTP/dITP diphosphatase -
  NFJ20_RS03760 (NFJ20_03755) yggU 779309..779599 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  NFJ20_RS03765 (NFJ20_03760) - 779596..780162 (-) 567 WP_003825417.1 YggT family protein -
  NFJ20_RS03770 (NFJ20_03765) - 780181..780885 (-) 705 WP_003838215.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFJ20_RS03775 (NFJ20_03770) pilT 780903..781883 (+) 981 WP_279274269.1 type IV pilus twitching motility protein PilT Machinery gene
  NFJ20_RS03780 (NFJ20_03775) ruvX 781880..782296 (-) 417 WP_003027087.1 Holliday junction resolvase RuvX -
  NFJ20_RS03785 (NFJ20_03780) - 782296..782859 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  NFJ20_RS03790 (NFJ20_03785) gshB 783035..783982 (-) 948 WP_003027083.1 glutathione synthase -
  NFJ20_RS03795 (NFJ20_03790) rsmE 784002..784733 (-) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFJ20_RS03800 (NFJ20_03795) endA 784808..785515 (-) 708 WP_016150969.1 deoxyribonuclease I -
  NFJ20_RS03805 (NFJ20_03800) - 785610..786107 (-) 498 WP_003838223.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35951.05 Da        Isoelectric Point: 6.4979

>NTDB_id=698498 NFJ20_RS03775 WP_279274269.1 780903..781883(+) (pilT) [Citrobacter freundii strain RHB02-E3-C01]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRRGKVESAPFTTPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRA
SAFVHLQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGAHCASFATGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQHR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698498 NFJ20_RS03775 WP_279274269.1 780903..781883(+) (pilT) [Citrobacter freundii strain RHB02-E3-C01]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCCGCGCG
TTGGCGCAGGCGTGGGAAAGTCGAAAGCGCACCGTTTACCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCTGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAACGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGCACATTGCGCCTCTTTCGCCACCGGTTTGCG
CGGTGCGCTACGAGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCACGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGATAGCCTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTTTACAGCATCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.938

98.16

0.451
  pilT Legionella pneumophila strain Lp02

45.938

98.16

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

39.062

98.16

0.383
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365