Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFJ54_RS03780 Genome accession   NZ_CP099260
Coordinates   775751..776731 (+) Length   326 a.a.
NCBI ID   WP_015369731.1    Uniprot ID   A0AAW9E0G2
Organism   Klebsiella aerogenes strain RHB08-SO-C04     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 770751..781731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ54_RS03750 (NFJ54_03750) - 771081..772127 (-) 1047 WP_015369725.1 sugar ABC transporter substrate-binding protein -
  NFJ54_RS03755 (NFJ54_03755) hemW 772421..773560 (-) 1140 WP_193128867.1 radical SAM family heme chaperone HemW -
  NFJ54_RS03760 (NFJ54_03760) - 773553..774146 (-) 594 WP_015369727.1 XTP/dITP diphosphatase -
  NFJ54_RS03765 (NFJ54_03765) yggU 774159..774449 (-) 291 WP_015369728.1 DUF167 family protein YggU -
  NFJ54_RS03770 (NFJ54_03770) - 774446..775012 (-) 567 WP_015369729.1 YggT family protein -
  NFJ54_RS03775 (NFJ54_03775) - 775030..775734 (-) 705 WP_046883094.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFJ54_RS03780 (NFJ54_03780) pilT 775751..776731 (+) 981 WP_015369731.1 type IV pilus twitching motility protein PilT Machinery gene
  NFJ54_RS03785 (NFJ54_03785) - 777082..777735 (+) 654 WP_015369732.1 LuxR C-terminal-related transcriptional regulator -
  NFJ54_RS03790 (NFJ54_03790) ruvX 777983..778399 (-) 417 WP_015369733.1 Holliday junction resolvase RuvX -
  NFJ54_RS03795 (NFJ54_03795) - 778399..778962 (-) 564 WP_015369734.1 YqgE/AlgH family protein -
  NFJ54_RS03800 (NFJ54_03800) gshB 779095..780045 (-) 951 WP_015369735.1 glutathione synthase -
  NFJ54_RS03805 (NFJ54_03805) rsmE 780094..780825 (-) 732 WP_015369736.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFJ54_RS03810 (NFJ54_03810) endA 780914..781612 (-) 699 WP_279281554.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35377.49 Da        Isoelectric Point: 6.5694

>NTDB_id=698454 NFJ54_RS03780 WP_015369731.1 775751..776731(+) (pilT) [Klebsiella aerogenes strain RHB08-SO-C04]
MELEEIVALSVKHNVSDLHLCSAYPPRWRRLGKLEPAPFTSPDVEEILTRWLNDPQLAQWRADGQVDFALALSDGPRLRA
SAFAHARGLSLALRLLPQRCPQLGDLGAPAALSELLNEESGLILVTGATGSGKSTTLAAMVDYLNQHRHGHILTLEDPVE
FIHASRNCLIQQREIGRHCSSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRSAAQAVERLVDV
FPAEEKEPVRSQLAGSLCAVAAQKLLPNASGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTSQQAGMQTFAQSFQQR
ALAGEL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698454 NFJ54_RS03780 WP_015369731.1 775751..776731(+) (pilT) [Klebsiella aerogenes strain RHB08-SO-C04]
ATGGAGCTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGCGCGTATCCGCCGCG
CTGGCGTCGTTTGGGAAAGCTTGAGCCTGCACCGTTTACTTCGCCGGATGTTGAGGAAATCCTCACTCGCTGGCTTAACG
ACCCACAGTTGGCGCAGTGGCGTGCCGATGGCCAGGTGGACTTTGCCCTGGCCCTCTCAGATGGGCCGCGGCTGCGTGCC
AGCGCTTTTGCCCACGCGCGCGGCTTGTCGTTGGCGCTGCGTCTTTTGCCGCAGCGATGCCCGCAGTTGGGTGATTTGGG
CGCGCCCGCCGCGCTTAGCGAACTGTTAAATGAAGAGAGTGGTTTGATCCTGGTTACCGGCGCTACCGGTAGCGGCAAGT
CGACCACCCTGGCGGCGATGGTGGACTACCTCAATCAACATCGCCATGGTCATATTTTAACCCTTGAGGATCCCGTGGAG
TTTATTCACGCCAGCAGGAACTGCCTGATCCAGCAGCGCGAAATCGGCCGCCATTGCTCCTCCTTCGCGGCGGCGCTACG
CGTGGCGCTGCGTCAGGACCCGGACGTGATCTTATTAGGTGAGCTACGCGATAGCGAAACCATCCGCCTGGCGCTGACCG
CGGCCGAAACCGGGCACCTGGTGATGGCGACGCTGCATACCCGCAGCGCGGCGCAGGCCGTCGAGCGGCTGGTGGATGTG
TTCCCGGCGGAAGAAAAAGAGCCGGTTCGCAGTCAGCTGGCAGGGAGCTTATGCGCCGTGGCGGCGCAAAAATTGTTGCC
AAATGCTTCCGGCGGCCGGGTTGCGCTATACGAACTTTTAGTGAACACGCCAGCGGTGGCAAATTTGATCCGCGAAGGAA
AAACGCATCAGCTACCCGGCGTGATGCAAACCAGTCAACAGGCAGGAATGCAAACCTTTGCGCAGAGTTTTCAACAACGT
GCGTTAGCAGGTGAGTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.795

98.773

0.521
  pilT Vibrio cholerae strain A1552

52.795

98.773

0.521
  pilT Neisseria meningitidis 8013

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.686

97.546

0.485
  pilT Legionella pneumophila strain Lp02

49.686

97.546

0.485
  pilT Neisseria gonorrhoeae MS11

49.068

98.773

0.485
  pilT Acinetobacter nosocomialis M2

47.826

98.773

0.472
  pilT Acinetobacter baumannii D1279779

47.826

98.773

0.472
  pilT Acinetobacter baumannii strain A118

47.826

98.773

0.472
  pilT Pseudomonas stutzeri DSM 10701

48.734

96.933

0.472
  pilT Acinetobacter baylyi ADP1

47.516

98.773

0.469
  pilT Pseudomonas aeruginosa PAK

47.468

96.933

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.426

97.239

0.442
  pilU Pseudomonas stutzeri DSM 10701

41.515

100

0.42
  pilU Vibrio cholerae strain A1552

42.236

98.773

0.417
  pilU Acinetobacter baylyi ADP1

38.318

98.466

0.377