Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFJ76_RS03720 Genome accession   NZ_CP099222
Coordinates   759008..759988 (+) Length   326 a.a.
NCBI ID   WP_117342644.1    Uniprot ID   A0AAU7GQ48
Organism   Citrobacter freundii strain RHB13-SO-C05     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 754008..764988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFJ76_RS03690 (NFJ76_03690) ansB 754513..755559 (+) 1047 WP_096755739.1 L-asparaginase 2 -
  NFJ76_RS03695 (NFJ76_03695) hemW 755684..756820 (-) 1137 WP_279271562.1 radical SAM family heme chaperone HemW -
  NFJ76_RS03700 (NFJ76_03700) - 756813..757406 (-) 594 WP_115257510.1 XTP/dITP diphosphatase -
  NFJ76_RS03705 (NFJ76_03705) yggU 757414..757704 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  NFJ76_RS03710 (NFJ76_03710) - 757701..758267 (-) 567 WP_096755742.1 YggT family protein -
  NFJ76_RS03715 (NFJ76_03715) - 758286..758990 (-) 705 WP_096755743.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFJ76_RS03720 (NFJ76_03720) pilT 759008..759988 (+) 981 WP_117342644.1 type IV pilus twitching motility protein PilT Machinery gene
  NFJ76_RS03725 (NFJ76_03725) ruvX 759998..760414 (-) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  NFJ76_RS03730 (NFJ76_03730) - 760414..760977 (-) 564 WP_096755746.1 YqgE/AlgH family protein -
  NFJ76_RS03735 (NFJ76_03735) gshB 761153..762100 (-) 948 WP_117342642.1 glutathione synthase -
  NFJ76_RS03740 (NFJ76_03740) rsmE 762113..762844 (-) 732 WP_117342641.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFJ76_RS03745 (NFJ76_03745) endA 762919..763626 (-) 708 WP_117342640.1 deoxyribonuclease I -
  NFJ76_RS03750 (NFJ76_03750) - 763864..764739 (+) 876 WP_279271563.1 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35800.97 Da        Isoelectric Point: 6.4952

>NTDB_id=698391 NFJ76_RS03720 WP_117342644.1 759008..759988(+) (pilT) [Citrobacter freundii strain RHB13-SO-C05]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTAPDVEKLLMLWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHQQGTSLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
YRYTSQRCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSNLIREGKTHQLPGVIQTGQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698391 NFJ76_RS03720 WP_117342644.1 759008..759988(+) (pilT) [Citrobacter freundii strain RHB13-SO-C05]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGCGCCTGACGTGGAGAAGCTGCTGATGTTATGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCAGCAGGGAACGTCACTGGCGCTGAGACTGCTGCCGCTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGCTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGACGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TACCGCTATACCAGCCAGCGTTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCTGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGTTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTTGAACTGCTGGTCAATACGTCGGCTGTGAGTAATCTGATCCGTGAAGGGA
AAACCCACCAGCTACCCGGCGTGATACAAACCGGGCAACAGGCGGGTATGCAAACATTTGCCCAGAGCCTGCAGCAGCGT
CAGGCGCAAGGCAGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Legionella pneumophila strain ERS1305867

46.584

98.773

0.46
  pilT Legionella pneumophila strain Lp02

46.584

98.773

0.46
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.396

97.546

0.423
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371