Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFK47_RS03775 Genome accession   NZ_CP099128
Coordinates   768316..769296 (+) Length   326 a.a.
NCBI ID   WP_032937406.1    Uniprot ID   A0A0J1NJ25
Organism   Citrobacter freundii strain RHB31-E4-C06     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 763316..774296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFK47_RS03745 (NFK47_03735) ansB 763830..764876 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  NFK47_RS03750 (NFK47_03740) hemW 764992..766128 (-) 1137 WP_032937402.1 radical SAM family heme chaperone HemW -
  NFK47_RS03755 (NFK47_03745) - 766121..766714 (-) 594 WP_003027104.1 XTP/dITP diphosphatase -
  NFK47_RS03760 (NFK47_03750) yggU 766722..767012 (-) 291 WP_032937404.1 DUF167 family protein YggU -
  NFK47_RS03765 (NFK47_03755) - 767009..767575 (-) 567 WP_003825417.1 YggT family protein -
  NFK47_RS03770 (NFK47_03760) - 767594..768298 (-) 705 WP_003838215.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFK47_RS03775 (NFK47_03765) pilT 768316..769296 (+) 981 WP_032937406.1 type IV pilus twitching motility protein PilT Machinery gene
  NFK47_RS03780 (NFK47_03770) ruvX 769293..769709 (-) 417 WP_032937474.1 Holliday junction resolvase RuvX -
  NFK47_RS03785 (NFK47_03775) - 769709..770272 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  NFK47_RS03790 (NFK47_03780) gshB 770384..771331 (-) 948 WP_003027083.1 glutathione synthase -
  NFK47_RS03795 (NFK47_03785) rsmE 771351..772082 (-) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFK47_RS03800 (NFK47_03790) endA 772157..772864 (-) 708 WP_016150969.1 deoxyribonuclease I -
  NFK47_RS03805 (NFK47_03795) - 772959..773456 (-) 498 WP_279278766.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35940.07 Da        Isoelectric Point: 6.4255

>NTDB_id=698322 NFK47_RS03775 WP_032937406.1 768316..769296(+) (pilT) [Citrobacter freundii strain RHB31-E4-C06]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRRGKVESAPFTTPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRV
SAFVHLQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGAHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698322 NFK47_RS03775 WP_032937406.1 768316..769296(+) (pilT) [Citrobacter freundii strain RHB31-E4-C06]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCCGCGCG
TTGGCGCAGGCGTGGAAAAGTCGAAAGCGCACCGTTTACCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGTC
AGCGCATTTGTCCATCTGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAATGGGTTGATTCTGGTGACAGGTGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCGCCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGTTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTACTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGTTTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J1NJ25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368