Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NFL15_RS03800 Genome accession   NZ_CP099037
Coordinates   777893..778873 (+) Length   326 a.a.
NCBI ID   WP_200011500.1    Uniprot ID   -
Organism   Citrobacter freundii strain RHB44-SO-C05     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 772893..783873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFL15_RS03770 (NFL15_03755) ansB 773407..774453 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  NFL15_RS03775 (NFL15_03760) hemW 774569..775705 (-) 1137 WP_032937402.1 radical SAM family heme chaperone HemW -
  NFL15_RS03780 (NFL15_03765) - 775698..776291 (-) 594 WP_003027104.1 XTP/dITP diphosphatase -
  NFL15_RS03785 (NFL15_03770) yggU 776299..776589 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  NFL15_RS03790 (NFL15_03775) - 776586..777152 (-) 567 WP_003825417.1 YggT family protein -
  NFL15_RS03795 (NFL15_03780) - 777171..777875 (-) 705 WP_049015284.1 YggS family pyridoxal phosphate-dependent enzyme -
  NFL15_RS03800 (NFL15_03785) pilT 777893..778873 (+) 981 WP_200011500.1 type IV pilus twitching motility protein PilT Machinery gene
  NFL15_RS03805 (NFL15_03790) ruvX 778870..779286 (-) 417 WP_006686843.1 Holliday junction resolvase RuvX -
  NFL15_RS03810 (NFL15_03795) - 779286..779849 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  NFL15_RS03815 (NFL15_03800) gshB 780025..780972 (-) 948 WP_003027083.1 glutathione synthase -
  NFL15_RS03820 (NFL15_03805) rsmE 780992..781723 (-) 732 WP_279279147.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NFL15_RS03825 (NFL15_03810) endA 781798..782505 (-) 708 WP_174328941.1 deoxyribonuclease I -
  NFL15_RS03830 (NFL15_03815) - 782600..783097 (-) 498 WP_202581003.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35985.06 Da        Isoelectric Point: 6.4255

>NTDB_id=698299 NFL15_RS03800 WP_200011500.1 777893..778873(+) (pilT) [Citrobacter freundii strain RHB44-SO-C05]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRRGKVESAPFTTPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRA
SAFVHQQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGAHCASFATGLRGVLREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=698299 NFL15_RS03800 WP_200011500.1 777893..778873(+) (pilT) [Citrobacter freundii strain RHB44-SO-C05]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCCGCGCG
TTGGCGCAGGCGTGGAAAAGTCGAAAGCGCACCGTTTACCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCAGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAATGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCACCGGTTTGCG
CGGTGTGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAAACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTACTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGTTTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain ERS1305867

45.342

98.773

0.448
  pilT Legionella pneumophila strain Lp02

45.342

98.773

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.009

97.239

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365