Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NDX50_RS03690 Genome accession   NZ_CP098757
Coordinates   755538..756518 (-) Length   326 a.a.
NCBI ID   WP_004105710.1    Uniprot ID   A0A9P0U2X2
Organism   Klebsiella oxytoca strain AHC-6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 750538..761518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDX50_RS03660 (NDX50_03655) endA 750826..751533 (+) 708 WP_004115375.1 deoxyribonuclease I -
  NDX50_RS03665 (NDX50_03660) rsmE 751625..752356 (+) 732 WP_064356377.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NDX50_RS03670 (NDX50_03665) gshB 752377..753324 (+) 948 WP_004105704.1 glutathione synthase -
  NDX50_RS03675 (NDX50_03670) - 753501..754064 (+) 564 WP_224314328.1 YqgE/AlgH family protein -
  NDX50_RS03680 (NDX50_03675) ruvX 754064..754480 (+) 417 WP_004105707.1 Holliday junction resolvase RuvX -
  NDX50_RS03685 (NDX50_03680) - 754536..755189 (-) 654 WP_064356378.1 LuxR C-terminal-related transcriptional regulator -
  NDX50_RS03690 (NDX50_03685) pilT 755538..756518 (-) 981 WP_004105710.1 type IV pilus twitching motility protein PilT Machinery gene
  NDX50_RS03695 (NDX50_03690) - 756535..757239 (+) 705 WP_016807917.1 YggS family pyridoxal phosphate-dependent enzyme -
  NDX50_RS03700 (NDX50_03695) - 757257..757823 (+) 567 WP_004105714.1 YggT family protein -
  NDX50_RS03705 (NDX50_03700) yggU 757820..758110 (+) 291 WP_004105716.1 DUF167 family protein YggU -
  NDX50_RS03710 (NDX50_03705) - 758123..758716 (+) 594 WP_004105717.1 XTP/dITP diphosphatase -
  NDX50_RS03715 (NDX50_03710) hemW 758709..759845 (+) 1137 WP_004105720.1 radical SAM family heme chaperone HemW -
  NDX50_RS03720 (NDX50_03715) - 760150..761136 (+) 987 WP_064397071.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35124.23 Da        Isoelectric Point: 6.7168

>NTDB_id=697754 NDX50_RS03690 WP_004105710.1 755538..756518(-) (pilT) [Klebsiella oxytoca strain AHC-6]
MELEEIVALSVKHNVSDLHLCNASAPRWRRQGKLEPAPFTSPDISKILHDWLNAEQLAQWQASGQVDFALAPAGGPRLRA
SAFAHSRGLSLALRLLAETCPQLEALGVPAALSELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLNGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFAAALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRSQLAGSLCAVLAQKLFPDAAGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQSGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=697754 NDX50_RS03690 WP_004105710.1 755538..756518(-) (pilT) [Klebsiella oxytoca strain AHC-6]
ATGGAACTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCTTCAGCGCCGCG
CTGGCGTCGCCAGGGAAAGCTGGAGCCTGCGCCTTTTACCTCCCCTGATATCAGCAAAATACTCCATGACTGGCTCAACG
CCGAGCAGCTCGCGCAGTGGCAGGCCAGCGGGCAGGTCGATTTCGCTTTAGCGCCGGCTGGCGGGCCACGCCTTCGCGCC
AGCGCATTTGCCCACAGCCGCGGCCTGTCGCTGGCGCTGCGTCTGCTGGCGGAAACCTGCCCGCAGCTGGAGGCGCTCGG
TGTACCAGCCGCCTTAAGCGAGCTGCTCAATGAGGAGAGCGGGCTTATCCTCGTCACGGGCGCCACCGGCAGCGGGAAAT
CAACTACCCTGGCGGCGATGGTGGGCTATCTTAATCAGCGCCTCAATGGTCATATCCTGACGCTGGAAGATCCGGTCGAA
TTTATTCACCACAGCGAGCGTTGCCTGATCCAGCAGCGGGAAATCGGCCGCCACTGCCCCTCCTTTGCCGCGGCGTTACG
CGCTGCGCTGCGTCAGGATCCGGACGTCATTCTGTTAGGGGAACTGCGCGATAGCGAAACTATCCGCCTGGCACTTACGG
CGGCGGAGACCGGACATCTGGTTATGGCGACGCTGCATACGCGCGGCGCGGCGCAGGCGGTTGAAAGGCTGGTGGATGTT
TTTCCCGCCCAGGAGAAAGAGCAGGTTCGCAGCCAGCTGGCGGGAAGTTTGTGCGCGGTACTGGCACAAAAATTGTTTCC
GGATGCTGCAGGCGGCAGAGTTGCGCTATACGAGCTTTTAGTTAATACCCCAGCAGTGGCGAATTTGATCCGGGAGGGAA
AAACGCATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAATCTGGCATGCAAACATTTACGCAAAGCTTTCAGCAACGT
ACGGCAGCGGGTCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.152

100

0.506
  pilT Vibrio cholerae strain A1552

50.152

100

0.506
  pilT Neisseria meningitidis 8013

48.182

100

0.488
  pilT Neisseria gonorrhoeae MS11

47.879

100

0.485
  pilT Legionella pneumophila strain Lp02

48.428

97.546

0.472
  pilT Legionella pneumophila strain ERS1305867

48.428

97.546

0.472
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter baumannii strain A118

45.152

100

0.457
  pilT Acinetobacter baumannii D1279779

45.152

100

0.457
  pilT Acinetobacter baylyi ADP1

45.289

100

0.457
  pilT Acinetobacter nosocomialis M2

44.848

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.463

100

0.417
  pilU Vibrio cholerae strain A1552

41.304

98.773

0.408
  pilU Acinetobacter baylyi ADP1

37.888

98.773

0.374