Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NDK47_RS21385 Genome accession   NZ_CP098755
Coordinates   4350089..4351132 (-) Length   347 a.a.
NCBI ID   WP_305883345.1    Uniprot ID   -
Organism   Brevibacillus ruminantium strain BB3-R1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4345089..4356132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDK47_RS21360 (NDK47_21360) - 4345458..4346105 (-) 648 WP_251871777.1 PilN domain-containing protein -
  NDK47_RS21365 (NDK47_21365) pilM 4346111..4347136 (-) 1026 WP_251871778.1 type IV pilus biogenesis protein PilM -
  NDK47_RS21370 (NDK47_21370) - 4347379..4348137 (-) 759 WP_251871779.1 A24 family peptidase -
  NDK47_RS21375 (NDK47_21375) - 4348400..4348846 (-) 447 WP_251871780.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NDK47_RS21380 (NDK47_21380) - 4348880..4350088 (-) 1209 WP_251871781.1 type II secretion system F family protein -
  NDK47_RS21385 (NDK47_21385) pilT 4350089..4351132 (-) 1044 WP_305883345.1 type IV pilus twitching motility protein PilT Machinery gene
  NDK47_RS21390 (NDK47_21390) - 4351173..4352828 (-) 1656 WP_251871782.1 GspE/PulE family protein -
  NDK47_RS21395 (NDK47_21395) - 4352834..4354513 (-) 1680 WP_251871783.1 VanW family protein -
  NDK47_RS21400 (NDK47_21400) - 4354522..4355334 (-) 813 WP_251871784.1 hypothetical protein -
  NDK47_RS21405 (NDK47_21405) - 4355579..4356094 (+) 516 WP_251871785.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38743.76 Da        Isoelectric Point: 7.0498

>NTDB_id=697736 NDK47_RS21385 WP_305883345.1 4350089..4351132(-) (pilT) [Brevibacillus ruminantium strain BB3-R1]
MAGVEIKKLLRYAFEKKASDLHVTVGTPPVFRIDGELRRLDIPPLAPGDTEEMAQELIPQHLYQSFVEKGELDFSYGLPG
VSRFRINAYHQRGCIGIVARVIPAGIPTLEELRLPEVIRKFCHKPQGLLLVTGPTGSGKSTTLAAMIDYINRTMRKHIVT
LEDPIEYLHKHQLSMVNQREIGFDTNDFSSGLRSALRQDPDVILVGEMRDLETIQTAVTAAETGHLVLATLHTTDAPKTI
DRIIDVFPGHRHSQIRMQLASVLVGIISQRLFPRVGGGRAVATEILVHTHAIANLIRIEKVHQIKSMMQTGRELGMHTME
SSVTELVERGEITQEAAQYYLSERALE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=697736 NDK47_RS21385 WP_305883345.1 4350089..4351132(-) (pilT) [Brevibacillus ruminantium strain BB3-R1]
ATGGCAGGGGTGGAAATCAAAAAGCTGCTCAGGTACGCCTTTGAAAAAAAAGCATCCGATTTGCATGTAACCGTGGGGAC
GCCCCCTGTCTTTCGAATCGACGGTGAGCTGCGCCGTCTGGATATCCCGCCGCTTGCGCCAGGCGATACGGAAGAGATGG
CCCAGGAACTGATCCCGCAGCATTTGTACCAGTCGTTTGTGGAAAAAGGAGAGCTCGATTTTTCCTATGGATTGCCGGGT
GTCTCCCGCTTCCGGATCAATGCCTATCACCAGCGCGGCTGTATTGGTATCGTGGCGCGGGTCATTCCCGCCGGGATTCC
GACGCTGGAGGAGCTTCGTTTGCCGGAGGTCATCCGGAAATTTTGCCACAAGCCGCAGGGGCTTCTCCTGGTCACCGGTC
CGACGGGAAGCGGGAAGTCGACGACGCTTGCTGCGATGATCGACTATATCAACCGGACGATGCGAAAACATATCGTCACG
CTGGAAGACCCGATCGAGTATCTGCACAAGCATCAGCTTTCCATGGTGAACCAGCGGGAGATCGGTTTTGATACCAACGA
TTTTTCCAGCGGGTTGCGCTCCGCCCTGCGGCAGGACCCCGACGTGATTCTGGTCGGCGAGATGCGCGATCTGGAAACGA
TCCAGACAGCCGTCACAGCAGCGGAGACCGGCCATCTGGTGCTGGCTACCCTCCATACGACAGATGCTCCGAAGACGATT
GACCGGATTATCGATGTGTTTCCGGGACATCGTCATTCCCAGATCCGCATGCAGTTGGCCTCGGTATTGGTGGGGATTAT
CTCGCAACGGCTGTTTCCCCGCGTGGGAGGAGGACGTGCAGTTGCCACCGAAATTCTCGTGCACACCCATGCGATTGCCA
ACCTGATCCGCATCGAAAAGGTGCATCAGATCAAAAGCATGATGCAAACCGGCCGCGAGCTGGGCATGCACACCATGGAA
TCCTCGGTCACAGAGCTGGTAGAGAGAGGAGAAATCACTCAGGAGGCTGCGCAGTACTATTTGAGTGAAAGGGCGCTGGA
GTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.083

96.83

0.504
  pilT Vibrio cholerae strain A1552

52.083

96.83

0.504
  pilT Pseudomonas stutzeri DSM 10701

51.045

96.542

0.493
  pilT Pseudomonas aeruginosa PAK

51.045

96.542

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.746

96.542

0.49
  pilT Acinetobacter nosocomialis M2

49.851

96.542

0.481
  pilT Acinetobacter baumannii strain A118

49.851

96.542

0.481
  pilT Acinetobacter baumannii D1279779

49.851

96.542

0.481
  pilT Acinetobacter baylyi ADP1

49.552

96.542

0.478
  pilT Neisseria gonorrhoeae MS11

48.214

96.83

0.467
  pilT Neisseria meningitidis 8013

48.214

96.83

0.467
  pilT Legionella pneumophila strain ERS1305867

47.605

96.254

0.458
  pilT Legionella pneumophila strain Lp02

47.605

96.254

0.458
  pilU Pseudomonas stutzeri DSM 10701

43.582

96.542

0.421
  pilU Vibrio cholerae strain A1552

40

96.542

0.386
  pilU Acinetobacter baylyi ADP1

37.952

95.677

0.363