Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MAMMFC1_RS19915 Genome accession   NZ_AP018449
Coordinates   4334641..4335699 (-) Length   352 a.a.
NCBI ID   WP_126310817.1    Uniprot ID   -
Organism   Methylomusa anaerophila strain MMFC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4329641..4340699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MAMMFC1_RS19875 (MAMMFC1_04028) - 4329831..4330124 (-) 294 WP_126310162.1 late competence development ComFB family protein -
  MAMMFC1_RS19880 (MAMMFC1_04029) - 4330194..4330652 (-) 459 WP_126310163.1 hypothetical protein -
  MAMMFC1_RS19885 (MAMMFC1_04030) - 4330688..4331218 (-) 531 WP_126310164.1 PilW family protein -
  MAMMFC1_RS19890 (MAMMFC1_04031) - 4331241..4331588 (-) 348 WP_145987662.1 hypothetical protein -
  MAMMFC1_RS19895 (MAMMFC1_04032) - 4331629..4332087 (-) 459 WP_126310166.1 type II secretion system protein -
  MAMMFC1_RS19900 (MAMMFC1_04033) - 4332098..4332862 (-) 765 WP_126310167.1 A24 family peptidase -
  MAMMFC1_RS19905 (MAMMFC1_04034) - 4332977..4333384 (-) 408 WP_197723853.1 type II secretion system protein -
  MAMMFC1_RS19910 (MAMMFC1_04035) - 4333401..4334624 (-) 1224 WP_126310168.1 type II secretion system F family protein -
  MAMMFC1_RS19915 (MAMMFC1_04036) pilT 4334641..4335699 (-) 1059 WP_126310817.1 type IV pilus twitching motility protein PilT Machinery gene
  MAMMFC1_RS19920 (MAMMFC1_04037) - 4335724..4337247 (-) 1524 WP_126310169.1 GspE/PulE family protein -
  MAMMFC1_RS19925 (MAMMFC1_04038) - 4337439..4338305 (-) 867 WP_126310170.1 shikimate dehydrogenase -
  MAMMFC1_RS19930 (MAMMFC1_04039) - 4338401..4339072 (-) 672 WP_126310171.1 hypothetical protein -
  MAMMFC1_RS19935 (MAMMFC1_04040) - 4339218..4340291 (+) 1074 WP_126310172.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39012.97 Da        Isoelectric Point: 6.9677

>NTDB_id=69739 MAMMFC1_RS19915 WP_126310817.1 4334641..4335699(-) (pilT) [Methylomusa anaerophila strain MMFC1]
MNSLLKQAVLLQASDIHFTVGIPPIARLKGSLTPIEHPVLTGEDMEALFSQIADTDQQSRFHQLGELDFSYALSGISRFR
VNAFKQRGTIAIAVRVVAERIPALKELGYPEILRTFTRKSRGLVLVTGPTGCGKSTTLAAMLDLINNERSCHIITLEDPI
EYLHHHKKSIVNQREINTDTISFASALRAVLREDPDVIMIGEMRDVETIGIALTAAETGHLVFATLHTGDAVQTVDRIVD
VFPPHQHRQVRIQLSLTLQGVIAQQLIPRCDNYDRVAALEVLVATPAVRNLIREGKTHQLASVIQTGAKMGMQAMDMSLK
NLYHRGIITYDEALSRAVDQEGFIRTINDKDC

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=69739 MAMMFC1_RS19915 WP_126310817.1 4334641..4335699(-) (pilT) [Methylomusa anaerophila strain MMFC1]
ATGAATTCTTTGTTAAAACAGGCGGTATTACTACAGGCTTCCGATATCCACTTTACGGTAGGCATCCCGCCTATTGCCAG
GCTTAAAGGCAGCTTGACGCCAATAGAGCATCCGGTACTTACCGGTGAAGACATGGAAGCACTATTTTCCCAGATAGCCG
ATACGGATCAGCAATCCCGATTTCACCAACTGGGTGAGTTGGATTTTTCTTATGCGTTATCAGGCATAAGCCGCTTTAGA
GTAAATGCCTTTAAACAAAGAGGAACTATAGCGATAGCCGTTCGGGTAGTGGCTGAGAGGATACCTGCTTTAAAGGAACT
GGGATACCCTGAGATTCTCAGGACTTTTACCCGTAAATCTCGTGGACTGGTCTTGGTTACCGGACCGACAGGGTGCGGCA
AATCTACTACCTTGGCTGCCATGCTTGATCTTATAAATAATGAACGGTCCTGCCATATTATTACCTTGGAAGATCCAATC
GAATATTTGCATCACCATAAGAAGAGCATAGTTAACCAGAGAGAAATAAACACTGACACTATTTCCTTTGCCAGTGCCCT
GCGGGCAGTGCTGCGGGAAGACCCTGACGTGATTATGATCGGGGAGATGCGTGATGTTGAAACCATTGGCATTGCTTTAA
CCGCTGCCGAAACCGGGCATCTGGTATTTGCAACTCTACACACCGGCGATGCTGTGCAAACTGTTGACCGGATTGTGGAT
GTTTTTCCGCCGCACCAACACCGGCAAGTACGTATTCAGTTGTCTTTAACTTTACAGGGAGTTATCGCCCAGCAGTTAAT
TCCACGTTGCGATAATTACGATAGGGTGGCAGCCCTGGAAGTCCTGGTGGCAACTCCGGCAGTGCGTAATCTGATCCGGG
AAGGCAAGACGCATCAATTGGCGTCTGTTATTCAAACCGGGGCAAAAATGGGGATGCAGGCTATGGATATGTCACTCAAA
AATCTTTATCACCGTGGAATTATCACCTATGATGAGGCGCTGTCAAGGGCTGTGGATCAAGAAGGATTTATCCGGACAAT
CAATGATAAAGACTGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(4-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.284

100

0.503
  pilT Neisseria meningitidis 8013

50.751

94.602

0.48
  pilT Acinetobacter baylyi ADP1

49.271

97.443

0.48
  pilT Pseudomonas aeruginosa PAK

50.453

94.034

0.474
  pilT Neisseria gonorrhoeae MS11

50

94.318

0.472
  pilT Pseudomonas stutzeri DSM 10701

50.151

94.034

0.472
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.249

94.602

0.466
  pilT Vibrio cholerae strain A1552

49.249

94.602

0.466
  pilT Acinetobacter nosocomialis M2

47.674

97.727

0.466
  pilT Acinetobacter baumannii strain A118

47.674

97.727

0.466
  pilT Acinetobacter baumannii D1279779

47.674

97.727

0.466
  pilT Legionella pneumophila strain Lp02

47.941

96.591

0.463
  pilT Legionella pneumophila strain ERS1305867

47.941

96.591

0.463
  pilU Pseudomonas stutzeri DSM 10701

42.216

94.886

0.401
  pilU Vibrio cholerae strain A1552

41.84

95.739

0.401
  pilU Acinetobacter baylyi ADP1

40.533

96.023

0.389

Multiple sequence alignment