Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ND012_RS13350 Genome accession   NZ_CP098710
Coordinates   2929020..2930045 (-) Length   341 a.a.
NCBI ID   WP_004719267.1    Uniprot ID   A0A085UB31
Organism   Yersinia ruckeri strain NVI-4479     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2924020..2935045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ND012_RS13325 (ND012_13315) gshB 2924304..2925263 (+) 960 WP_004719260.1 glutathione synthase -
  ND012_RS13330 (ND012_13320) - 2925378..2925941 (+) 564 WP_038240991.1 YqgE/AlgH family protein -
  ND012_RS13335 (ND012_13325) ruvX 2925941..2926366 (+) 426 WP_038240988.1 Holliday junction resolvase RuvX -
  ND012_RS13340 (ND012_13330) aguB 2926785..2927669 (+) 885 WP_004719263.1 N-carbamoylputrescine amidase -
  ND012_RS13345 (ND012_13335) aguA 2927672..2928757 (+) 1086 WP_045844286.1 agmatine deiminase -
  ND012_RS13350 (ND012_13340) pilT 2929020..2930045 (-) 1026 WP_004719267.1 type IV pilus twitching motility protein PilT Machinery gene
  ND012_RS13355 (ND012_13345) - 2930065..2930778 (+) 714 WP_004719269.1 YggS family pyridoxal phosphate-dependent enzyme -
  ND012_RS13360 (ND012_13350) proC 2930791..2931612 (+) 822 WP_004719272.1 pyrroline-5-carboxylate reductase -
  ND012_RS13365 (ND012_13355) - 2931716..2932270 (+) 555 WP_004719273.1 YggT family protein -
  ND012_RS13370 (ND012_13360) yggU 2932267..2932557 (+) 291 WP_038240982.1 DUF167 family protein YggU -
  ND012_RS13375 (ND012_13365) - 2932658..2933251 (+) 594 WP_004719276.1 XTP/dITP diphosphatase -
  ND012_RS13380 (ND012_13370) hemW 2933244..2934374 (+) 1131 WP_038240979.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37127.52 Da        Isoelectric Point: 6.7505

>NTDB_id=697223 ND012_RS13350 WP_004719267.1 2929020..2930045(-) (pilT) [Yersinia ruckeri strain NVI-4479]
MDFDEWVAASVKHNASDLHLCTGHYPVLRIAGDLHIFKHLTQVTPMWMENLAQQLLNEVHSLELQRHGQVDFSFSSSCGS
RLRGHFFRQQCGLSAAFRLIASQCPTLVDLEVPEVVHQLLPCESGLILVTGATGSGKSTTLAGMIGSINQTGGRHIITLE
DPVEFIHHSEGCLIQQRELGGHIASFGAGLKAALRQDPDIILLGELRDRETIALALTAAETGHLVLGTLHTRTAAQAVDR
LVDVFPAEEKTSVRSQLANSLMAVIAQKLVRRRGGGQVAVFETMIQTAAVSHLIREGKTHQLPGIMQTGGAMGMQTFEQG
LQQRQHQGLLPLAEDISQIHH

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=697223 ND012_RS13350 WP_004719267.1 2929020..2930045(-) (pilT) [Yersinia ruckeri strain NVI-4479]
ATGGATTTTGATGAATGGGTGGCGGCTAGTGTAAAGCATAACGCCTCTGATCTGCACCTGTGCACTGGCCATTACCCGGT
GCTGCGTATTGCTGGTGACCTGCATATTTTCAAGCATCTCACTCAGGTAACGCCGATGTGGATGGAAAATCTGGCTCAGC
AGCTATTAAATGAGGTTCATTCTCTGGAATTACAACGGCATGGACAGGTCGATTTTTCATTTTCATCATCTTGTGGATCA
AGATTGAGAGGGCATTTCTTTCGGCAGCAGTGCGGGCTTTCAGCCGCTTTTCGCTTGATTGCGTCCCAATGCCCAACATT
GGTTGATCTTGAAGTTCCGGAAGTTGTTCATCAATTATTGCCCTGTGAAAGTGGTTTGATTTTGGTAACGGGAGCCACGG
GTAGTGGGAAATCAACCACTCTGGCGGGGATGATTGGATCCATCAATCAAACGGGCGGGCGGCATATTATTACATTGGAA
GATCCGGTTGAGTTTATTCATCACAGTGAGGGTTGTCTGATTCAACAGCGGGAGTTGGGTGGACATATTGCTTCTTTTGG
CGCGGGGCTAAAAGCGGCACTAAGGCAGGATCCCGATATTATCTTGTTGGGAGAGCTACGAGATCGGGAAACTATTGCAC
TTGCGTTGACGGCGGCGGAAACCGGGCATTTGGTGTTGGGCACACTACATACCCGGACAGCCGCGCAGGCGGTGGATCGA
TTGGTTGATGTATTTCCAGCTGAAGAAAAAACCAGTGTGCGAAGCCAGCTAGCAAACAGCCTGATGGCGGTAATTGCGCA
AAAGCTGGTGCGGCGTCGCGGAGGAGGGCAGGTGGCGGTGTTTGAAACCATGATACAGACAGCGGCAGTTAGCCATTTGA
TACGCGAGGGAAAAACCCACCAACTACCTGGAATTATGCAAACCGGCGGTGCAATGGGAATGCAAACTTTTGAGCAGGGA
TTACAACAGCGTCAACATCAGGGATTATTGCCATTGGCGGAAGACATTAGCCAGATACATCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A085UB31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.558

99.413

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.558

99.413

0.493
  pilT Pseudomonas stutzeri DSM 10701

48.182

96.774

0.466
  pilT Neisseria gonorrhoeae MS11

48.036

97.067

0.466
  pilT Neisseria meningitidis 8013

48.036

97.067

0.466
  pilT Acinetobacter baylyi ADP1

46.471

99.707

0.463
  pilT Pseudomonas aeruginosa PAK

47.576

96.774

0.46
  pilT Legionella pneumophila strain ERS1305867

45.946

97.654

0.449
  pilT Legionella pneumophila strain Lp02

45.946

97.654

0.449
  pilT Acinetobacter nosocomialis M2

46.061

96.774

0.446
  pilT Acinetobacter baumannii strain A118

46.894

94.428

0.443
  pilT Acinetobacter baumannii D1279779

46.894

94.428

0.443
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.633

93.548

0.399
  pilU Pseudomonas stutzeri DSM 10701

39.21

96.481

0.378
  pilU Acinetobacter baylyi ADP1

37.651

97.361

0.367
  pilU Vibrio cholerae strain A1552

37.963

95.015

0.361