Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NBH00_RS14325 Genome accession   NZ_CP098502
Coordinates   2924204..2925307 (+) Length   367 a.a.
NCBI ID   WP_254569275.1    Uniprot ID   -
Organism   Paraconexibacter antarcticus strain 02-257     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2919204..2930307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBH00_RS14305 (NBH00_14305) ruvX 2919272..2919679 (+) 408 WP_254569271.1 Holliday junction resolvase RuvX -
  NBH00_RS14310 (NBH00_14310) mltG 2919688..2921496 (+) 1809 WP_254569272.1 endolytic transglycosylase MltG -
  NBH00_RS14315 (NBH00_14315) aroE 2921506..2922309 (+) 804 WP_254569273.1 shikimate dehydrogenase -
  NBH00_RS14320 (NBH00_14320) - 2922341..2924110 (+) 1770 WP_254569274.1 GspE/PulE family protein -
  NBH00_RS14325 (NBH00_14325) pilT 2924204..2925307 (+) 1104 WP_254569275.1 type IV pilus twitching motility protein PilT Machinery gene
  NBH00_RS14330 (NBH00_14330) - 2925311..2926519 (+) 1209 WP_254569276.1 type II secretion system F family protein -
  NBH00_RS14335 (NBH00_14335) purU 2926663..2927520 (+) 858 WP_254569277.1 formyltetrahydrofolate deformylase -
  NBH00_RS14340 (NBH00_14340) - 2927541..2928725 (-) 1185 WP_254569278.1 hydroxymethylglutaryl-CoA reductase -
  NBH00_RS14345 (NBH00_14345) - 2928757..2929620 (-) 864 WP_254569279.1 aldose 1-epimerase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 39787.50 Da        Isoelectric Point: 6.4294

>NTDB_id=696354 NBH00_RS14325 WP_254569275.1 2924204..2925307(+) (pilT) [Paraconexibacter antarcticus strain 02-257]
MRFDFADLLLDVLERRASDLHITAGSHPMVRVRGRLTPMDEYPVLDATETREIVYAILNNDQRQRLETDWQLDFAYAIPG
RARFRVNAYFQRGALGAAFRLIPAQITPIDDLGLPPVVHEMCNKPRGIVLVTGPTGSGKSTTLAAMVDEINATREEHILT
IEDPIEFLHGHRKCMVNQREVGADAHSFAAGLKAALREDPDVILVGEMRDLETISTALTAAETGHLVFATLHTQSAPSTI
DRVIDVFPAHQQQQIRVQLSIALQGVITQTLIPTADGAGRAVACEILVPTAAVRNLIREGKTHQIPSSMQTGSTVGMQTM
DAALAALVRAGKITQRVAETRSSTPDELRRILGAATLGGGVNYGAAA

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=696354 NBH00_RS14325 WP_254569275.1 2924204..2925307(+) (pilT) [Paraconexibacter antarcticus strain 02-257]
ATGCGCTTCGACTTCGCCGACCTGCTGCTGGACGTCCTGGAGCGCCGGGCGTCCGACCTCCACATCACCGCCGGCTCGCA
TCCGATGGTGCGCGTCCGCGGCCGGCTGACGCCGATGGACGAGTACCCGGTGCTCGACGCGACGGAGACACGGGAGATCG
TCTACGCGATCCTCAACAACGACCAGCGCCAGCGCCTGGAGACGGACTGGCAGCTCGACTTCGCGTACGCGATCCCGGGC
CGCGCCCGCTTCCGCGTGAACGCCTACTTCCAGCGCGGCGCGCTCGGGGCGGCCTTCCGCCTCATCCCCGCGCAGATCAC
GCCGATCGACGACCTCGGCCTGCCGCCGGTCGTCCACGAGATGTGCAACAAGCCCCGCGGCATCGTGCTCGTGACGGGCC
CGACGGGCTCGGGCAAGTCGACGACGCTCGCTGCGATGGTCGACGAGATCAACGCGACGCGCGAGGAGCACATCCTCACG
ATCGAGGACCCCATCGAGTTCCTCCACGGCCACCGCAAGTGCATGGTCAACCAGCGCGAGGTCGGGGCGGACGCCCACTC
CTTCGCCGCCGGCCTGAAGGCCGCCCTGCGCGAGGACCCCGACGTCATCCTCGTCGGCGAGATGCGCGACCTCGAGACGA
TCTCCACGGCGCTCACCGCGGCGGAGACCGGCCACCTCGTCTTCGCGACGCTCCACACGCAGAGCGCCCCGTCGACGATC
GACCGCGTCATCGACGTCTTCCCGGCCCACCAGCAGCAGCAGATCCGCGTCCAGCTGTCGATAGCCCTGCAGGGCGTCAT
CACCCAGACGCTCATCCCGACGGCGGACGGCGCCGGGCGCGCGGTCGCCTGCGAGATCCTCGTCCCCACCGCCGCGGTGC
GGAACCTCATCCGCGAGGGCAAGACCCACCAGATCCCGTCGTCGATGCAGACGGGCTCGACCGTGGGCATGCAGACGATG
GACGCGGCGCTCGCGGCGCTCGTCCGCGCGGGCAAGATCACCCAGCGCGTCGCGGAGACGCGGTCCTCGACCCCGGACGA
GCTCCGGCGGATCCTCGGGGCCGCGACGCTCGGCGGTGGCGTCAACTACGGAGCGGCGGCCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.151

99.455

0.529
  pilT Acinetobacter nosocomialis M2

52.89

94.278

0.499
  pilT Acinetobacter baumannii strain A118

52.89

94.278

0.499
  pilT Acinetobacter baumannii D1279779

52.89

94.278

0.499
  pilT Legionella pneumophila strain Lp02

54.328

91.281

0.496
  pilT Legionella pneumophila strain ERS1305867

54.328

91.281

0.496
  pilT Pseudomonas aeruginosa PAK

52.312

94.278

0.493
  pilT Acinetobacter baylyi ADP1

52.312

94.278

0.493
  pilT Pseudomonas stutzeri DSM 10701

52.023

94.278

0.49
  pilT Neisseria meningitidis 8013

50.585

93.188

0.471
  pilT Neisseria gonorrhoeae MS11

50.292

93.188

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.263

92.371

0.455
  pilT Vibrio cholerae strain A1552

49.263

92.371

0.455
  pilU Pseudomonas stutzeri DSM 10701

42.458

97.548

0.414
  pilU Acinetobacter baylyi ADP1

42.939

94.55

0.406
  pilU Vibrio cholerae strain A1552

39.888

97.003

0.387