Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   UNDKW_RS04580 Genome accession   NZ_AP018439
Coordinates   1014929..1015972 (-) Length   347 a.a.
NCBI ID   WP_162057760.1    Uniprot ID   -
Organism   Undibacterium sp. KW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1009929..1020972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UNDKW_RS04550 (UNDKW_0916) glcF 1010092..1011390 (+) 1299 WP_162057755.1 glycolate oxidase subunit GlcF -
  UNDKW_RS04555 (UNDKW_0917) - 1011394..1011684 (+) 291 WP_162057756.1 DUF2867 domain-containing protein -
  UNDKW_RS04560 (UNDKW_0918) - 1011699..1012832 (-) 1134 Protein_913 IS4 family transposase -
  UNDKW_RS04565 (UNDKW_0919) - 1013008..1013265 (+) 258 WP_162057757.1 DUF2867 domain-containing protein -
  UNDKW_RS04570 (UNDKW_0920) - 1013294..1013776 (-) 483 WP_162057758.1 glutathione peroxidase -
  UNDKW_RS04575 (UNDKW_0921) pilU 1013773..1014909 (-) 1137 WP_162057759.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  UNDKW_RS04580 (UNDKW_0922) pilT 1014929..1015972 (-) 1044 WP_162057760.1 type IV pilus twitching motility protein PilT Machinery gene
  UNDKW_RS04585 (UNDKW_0923) - 1016107..1016811 (+) 705 WP_162057761.1 YggS family pyridoxal phosphate-dependent enzyme -
  UNDKW_RS04590 (UNDKW_0924) proC 1016801..1017625 (+) 825 WP_232063235.1 pyrroline-5-carboxylate reductase -
  UNDKW_RS04595 (UNDKW_0925) - 1017646..1017981 (-) 336 WP_162057762.1 YqjK family protein -
  UNDKW_RS04600 (UNDKW_0926) - 1017985..1018374 (-) 390 WP_162039957.1 phage holin family protein -
  UNDKW_RS04605 (UNDKW_0927) - 1018390..1018701 (-) 312 WP_162039958.1 YqjD family protein -
  UNDKW_RS04610 (UNDKW_0928) ubiA 1018832..1019686 (-) 855 WP_370529123.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38067.57 Da        Isoelectric Point: 6.5767

>NTDB_id=69608 UNDKW_RS04580 WP_162057760.1 1014929..1015972(-) (pilT) [Undibacterium sp. KW1]
MDISELLAFSVKNNASDLHLSSGLPPMIRVHGDVRRINLPPLEHKDVHELIYDIMNDSQRKAYEDTLECDFSFSIPGLAR
FRVNAFNQERGAAAVMRTIPSKILSLEQLNAPKIFADLSLKPRGLVLVTGPTGSGKSTTLAGMVNHLNENEYGHILTVED
PIEFVHESKKCLINQREVGPHTMSFNNALRSALREDPDAILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRSMLSESLQAVISQTLLKTKDGSGRVAAHEIMLGTPAIRNLIREAKIAQMYSSIQTGSNLGMQTLDQN
LTDLVRRNVISVATARGAAKTPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=69608 UNDKW_RS04580 WP_162057760.1 1014929..1015972(-) (pilT) [Undibacterium sp. KW1]
ATGGACATTTCAGAACTGCTGGCGTTCTCCGTCAAGAACAATGCCTCCGACTTACACTTATCCTCCGGCTTGCCGCCCAT
GATACGGGTACATGGCGATGTCAGGCGCATTAATTTGCCACCTCTGGAGCATAAAGATGTCCATGAGTTGATCTATGACA
TCATGAATGACTCGCAGCGCAAAGCTTATGAAGACACGCTGGAGTGCGACTTCTCGTTCTCCATCCCTGGTCTGGCACGT
TTCCGCGTGAATGCGTTTAACCAGGAACGTGGTGCCGCAGCCGTCATGCGTACCATTCCGTCCAAGATTTTGTCGCTGGA
ACAGTTGAATGCGCCCAAAATCTTTGCTGATTTGTCACTAAAACCGCGTGGTCTGGTCCTGGTGACCGGCCCTACCGGTT
CGGGCAAATCCACGACACTGGCAGGCATGGTGAATCACCTCAATGAAAATGAGTATGGCCACATCCTGACGGTTGAAGAC
CCGATAGAATTTGTTCATGAATCCAAAAAATGCCTGATCAACCAACGTGAAGTCGGCCCGCATACGATGTCTTTCAACAA
CGCGCTGCGCTCTGCCCTGCGTGAAGATCCCGATGCCATCCTGGTCGGTGAGTTGCGTGATCTGGAAACCATACGTCTGG
CACTGACTGCTGCTGAAACCGGTCACTTGGTATTCGGCACCCTGCATACCTCATCTGCTGCCAAGACCATTGACCGTATT
GTTGACGTATTCCCGGCGGAAGAAAAAGAAATGGTGCGTTCGATGTTGTCAGAATCCCTGCAAGCGGTCATCTCGCAAAC
CCTGCTGAAAACCAAGGATGGCTCTGGCCGTGTGGCAGCGCATGAAATCATGCTGGGTACACCAGCGATACGCAACCTGA
TCCGCGAAGCCAAGATTGCGCAGATGTATTCGTCGATACAAACCGGTAGTAACCTGGGCATGCAGACACTGGATCAAAAT
CTGACTGATCTGGTGCGCCGCAATGTCATCTCGGTGGCGACTGCACGTGGTGCAGCCAAAACACCAGACAACTTCCCAGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

75.221

97.695

0.735
  pilT Legionella pneumophila strain ERS1305867

75.221

97.695

0.735
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70

95.101

0.666
  pilT Vibrio cholerae strain A1552

70

95.101

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Vibrio cholerae strain A1552

40.87

99.424

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment