Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NB069_RS18335 Genome accession   NZ_CP098325
Coordinates   3890461..3891438 (-) Length   325 a.a.
NCBI ID   WP_250585878.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain kcgeb_e1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3885461..3896438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NB069_RS18300 (NB069_18300) - 3885461..3885958 (+) 498 WP_250585864.1 SprT family zinc-dependent metalloprotease -
  NB069_RS18305 (NB069_18305) endA 3886053..3886760 (+) 708 WP_250585866.1 deoxyribonuclease I -
  NB069_RS18310 (NB069_18310) rsmE 3886874..3887605 (+) 732 WP_250585868.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NB069_RS18315 (NB069_18315) gshB 3887625..3888572 (+) 948 WP_250585870.1 glutathione synthase -
  NB069_RS18320 (NB069_18320) - 3888661..3889221 (+) 561 WP_250585872.1 YqgE/AlgH family protein -
  NB069_RS18325 (NB069_18325) ruvX 3889221..3889637 (+) 417 WP_250585874.1 Holliday junction resolvase RuvX -
  NB069_RS18330 (NB069_18330) - 3889677..3890366 (-) 690 WP_250585876.1 IclR family transcriptional regulator -
  NB069_RS18335 (NB069_18335) pilT 3890461..3891438 (-) 978 WP_250585878.1 type IV pilus twitching motility protein PilT Machinery gene
  NB069_RS18340 (NB069_18340) - 3891456..3892157 (+) 702 WP_250585880.1 YggS family pyridoxal phosphate-dependent enzyme -
  NB069_RS18345 (NB069_18345) - 3892179..3892745 (+) 567 WP_250585882.1 YggT family protein -
  NB069_RS18350 (NB069_18350) yggU 3892742..3893038 (+) 297 WP_250585884.1 DUF167 family protein YggU -
  NB069_RS18355 (NB069_18355) - 3893042..3893635 (+) 594 WP_250585886.1 XTP/dITP diphosphatase -
  NB069_RS18360 (NB069_18360) hemW 3893628..3894776 (+) 1149 WP_250585888.1 radical SAM family heme chaperone HemW -
  NB069_RS18365 (NB069_18365) - 3894833..3895195 (+) 363 WP_250585890.1 endonuclease domain-containing protein -
  NB069_RS18370 (NB069_18370) - 3895299..3896018 (-) 720 WP_250585892.1 DUF2884 domain-containing protein -
  NB069_RS18375 (NB069_18375) - 3896071..3896397 (-) 327 WP_039029792.1 YggL family protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35680.79 Da        Isoelectric Point: 7.0533

>NTDB_id=694951 NB069_RS18335 WP_250585878.1 3890461..3891438(-) (pilT) [Leclercia adecarboxylata strain kcgeb_e1]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMEFAPFPGPDVAMLLKSWLSDEQQGAWYAQGQVDFAVALESHRLRAS
AFAHTQGCSLALRLLAQQCPQLRTLGTPRAIPELLTNDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCPSFADGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDAF
PAQEKEPVRKQLAGSLRAVLAQKLERDNQQGRVALFELLVNTPAAANLIREGKTWQLPGVIQTGQQAGMQNFEQSLAERR
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=694951 NB069_RS18335 WP_250585878.1 3890461..3891438(-) (pilT) [Leclercia adecarboxylata strain kcgeb_e1]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCGCCACCTCG
CTGGCGGCGGCGCGGGAAAATGGAATTCGCCCCTTTTCCAGGGCCGGATGTCGCCATGCTGCTGAAAAGCTGGCTCAGCG
ATGAGCAGCAGGGAGCCTGGTACGCCCAGGGGCAGGTGGATTTTGCCGTCGCGCTGGAGAGCCACCGGCTGCGTGCCAGC
GCCTTTGCCCATACGCAAGGGTGTTCGCTGGCGCTGCGTCTGCTGGCACAGCAGTGTCCGCAGCTGCGGACCCTGGGGAC
GCCGCGGGCGATACCCGAGCTGCTGACCAACGATAACGGCCTGATTCTGGTGACCGGGGCAACCGGCAGTGGGAAATCCA
CCACCCTTGCGGCAATGGTCGATTTTCTTAATCACCATAGCGACGGGCATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAGCGCTGCCTGGTGCAGCAGAGGGAGATTGGCCTGCATTGCCCGTCGTTTGCCGACGGGCTAAGAGC
TGCCCTGCGTGAGGATCCGGATGTGATCCTGCTGGGCGAACTGCGTGACAGCGAGACAATCCGCCTTGCGCTTACCGCCG
CCGAGACGGGGCATCTGGTGCTGGCGACCCTGCATACCCGGGGAGCGGCTCAGGCGGTAGAGAGGCTGGTGGATGCCTTC
CCCGCGCAGGAAAAAGAGCCGGTGCGTAAGCAGCTGGCAGGTAGCCTGCGCGCCGTGCTGGCGCAAAAGCTCGAAAGGGA
TAACCAGCAGGGTCGCGTTGCGCTGTTTGAGCTGCTGGTGAATACCCCTGCCGCAGCAAATTTGATCCGGGAAGGTAAAA
CCTGGCAACTGCCCGGCGTGATCCAGACCGGGCAGCAGGCAGGCATGCAAAACTTCGAGCAGAGCCTTGCCGAGCGCAGG
GCGCAGGGGCGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.489
  pilT Vibrio cholerae strain A1552

48.624

100

0.489
  pilT Neisseria meningitidis 8013

47.256

100

0.477
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.474
  pilT Legionella pneumophila strain ERS1305867

47.205

99.077

0.468
  pilT Legionella pneumophila strain Lp02

47.205

99.077

0.468
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.465
  pilT Acinetobacter baylyi ADP1

45.566

100

0.458
  pilT Acinetobacter baumannii D1279779

45.566

100

0.458
  pilT Acinetobacter baumannii strain A118

45.566

100

0.458
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.455
  pilT Acinetobacter nosocomialis M2

45.938

98.462

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

99.077

0.425
  pilU Vibrio cholerae strain A1552

40.187

98.769

0.397
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.385
  pilU Acinetobacter baylyi ADP1

36.615

100

0.366