Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KFZ60_RS03960 Genome accession   NZ_CP098231
Coordinates   817175..818155 (+) Length   326 a.a.
NCBI ID   WP_088376388.1    Uniprot ID   -
Organism   Escherichia coli strain XJ34     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 812175..823155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFZ60_RS03930 (KFZ60_03925) yggM 812602..813609 (+) 1008 WP_088376386.1 DUF1202 family protein -
  KFZ60_RS03935 (KFZ60_03930) hemW 813852..814988 (-) 1137 WP_088376387.1 radical SAM family heme chaperone HemW -
  KFZ60_RS03940 (KFZ60_03935) rdgB 814981..815574 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  KFZ60_RS03945 (KFZ60_03940) yggU 815582..815872 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  KFZ60_RS03950 (KFZ60_03945) yggT 815869..816435 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  KFZ60_RS03955 (KFZ60_03950) yggS 816453..817157 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  KFZ60_RS03960 (KFZ60_03955) pilT 817175..818155 (+) 981 WP_088376388.1 type IV pilus twitching motility protein PilT Machinery gene
  KFZ60_RS03965 (KFZ60_03960) ruvX 818346..818762 (-) 417 WP_000017110.1 Holliday junction resolvase RuvX -
  KFZ60_RS03970 (KFZ60_03965) yqgE 818762..819325 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  KFZ60_RS03975 (KFZ60_03970) gshB 819434..820384 (-) 951 WP_000593273.1 glutathione synthase -
  KFZ60_RS03980 (KFZ60_03975) rsmE 820397..821128 (-) 732 WP_097756073.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KFZ60_RS03985 (KFZ60_03980) endA 821208..821915 (-) 708 WP_000286500.1 deoxyribonuclease I -
  KFZ60_RS03990 (KFZ60_03985) yggI 822010..822507 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36003.20 Da        Isoelectric Point: 5.5860

>NTDB_id=694562 KFZ60_RS03960 WP_088376388.1 817175..818155(+) (pilT) [Escherichia coli strain XJ34]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFSVSLTENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGTPPILPELLKSENGLILLTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=694562 KFZ60_RS03960 WP_088376388.1 817175..818155(+) (pilT) [Escherichia coli strain XJ34]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCAGACGTCGAAGAGCTACTACGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTTCCGTGTCGCTGACGGAAAACCAGCGATTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CACACCACCGATATTGCCGGAGTTACTCAAGAGCGAGAATGGCTTGATTCTGTTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTTGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACTGGGCAACAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.748

97.239

0.387
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371