Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NBY15_RS05020 Genome accession   NZ_CP098214
Coordinates   986909..987889 (+) Length   326 a.a.
NCBI ID   WP_021560921.1    Uniprot ID   -
Organism   Escherichia coli strain Z0117EC0036     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 981909..992889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY15_RS04990 (NBY15_04985) yggM 982510..983517 (+) 1008 WP_000745192.1 DUF1202 family protein -
  NBY15_RS04995 (NBY15_04990) hemW 983586..984722 (-) 1137 WP_021521020.1 radical SAM family heme chaperone HemW -
  NBY15_RS05000 (NBY15_04995) rdgB 984715..985308 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  NBY15_RS05005 (NBY15_05000) yggU 985316..985606 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  NBY15_RS05010 (NBY15_05005) yggT 985603..986169 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  NBY15_RS05015 (NBY15_05010) yggS 986187..986891 (-) 705 WP_000997805.1 pyridoxal phosphate homeostasis protein -
  NBY15_RS05020 (NBY15_05015) pilT 986909..987889 (+) 981 WP_021560921.1 type IV pilus twitching motility protein PilT Machinery gene
  NBY15_RS05025 (NBY15_05020) ruvX 988063..988479 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  NBY15_RS05030 (NBY15_05025) yqgE 988479..989042 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  NBY15_RS05035 (NBY15_05030) gshB 989151..990101 (-) 951 WP_000593260.1 glutathione synthase -
  NBY15_RS05040 (NBY15_05035) rsmE 990114..990845 (-) 732 WP_014640203.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NBY15_RS05045 (NBY15_05040) endA 990925..991632 (-) 708 WP_001305312.1 deoxyribonuclease I -
  NBY15_RS05050 (NBY15_05045) yggI 991727..992224 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35942.12 Da        Isoelectric Point: 5.7980

>NTDB_id=694430 NBY15_RS05020 WP_021560921.1 986909..987889(+) (pilT) [Escherichia coli strain Z0117EC0036]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
GAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=694430 NBY15_RS05020 WP_021560921.1 986909..987889(+) (pilT) [Escherichia coli strain Z0117EC0036]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
GGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(86-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362