Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NBE99_RS10205 Genome accession   NZ_CP098039
Coordinates   2122644..2123741 (+) Length   365 a.a.
NCBI ID   WP_250681974.1    Uniprot ID   -
Organism   Thermosynechococcus sp. HN-54     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2117644..2128741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBE99_RS10185 (NBE99_10185) - 2118779..2118973 (-) 195 WP_250681971.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  NBE99_RS10190 (NBE99_10190) - 2118974..2119186 (-) 213 WP_250681972.1 hypothetical protein -
  NBE99_RS10195 (NBE99_10195) - 2119493..2120455 (+) 963 WP_399371026.1 transposase -
  NBE99_RS10200 (NBE99_10200) pilB/pilB1 2120546..2122576 (+) 2031 WP_250681973.1 GspE/PulE family protein Machinery gene
  NBE99_RS10205 (NBE99_10205) pilT 2122644..2123741 (+) 1098 WP_250681974.1 type IV pilus twitching motility protein PilT Machinery gene
  NBE99_RS10210 (NBE99_10210) - 2123791..2125011 (+) 1221 WP_250681975.1 type II secretion system F family protein -
  NBE99_RS10215 (NBE99_10215) - 2125025..2125498 (+) 474 WP_250681976.1 J domain-containing protein -
  NBE99_RS10220 (NBE99_10220) - 2125495..2125767 (+) 273 WP_250681977.1 DUF3143 domain-containing protein -
  NBE99_RS10225 (NBE99_10225) - 2125775..2126113 (-) 339 WP_250681978.1 hypothetical protein -
  NBE99_RS10230 (NBE99_10230) - 2126420..2127571 (-) 1152 WP_250681979.1 cysteine desulfurase family protein -
  NBE99_RS10240 (NBE99_10240) - 2127847..2128218 (+) 372 WP_250681980.1 ferredoxin-thioredoxin reductase catalytic domain-containing protein -
  NBE99_RS13485 - 2128215..2128475 (-) 261 WP_399371074.1 spore coat protein U domain-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40306.38 Da        Isoelectric Point: 6.7579

>NTDB_id=693808 NBE99_RS10205 WP_250681974.1 2122644..2123741(+) (pilT) [Thermosynechococcus sp. HN-54]
MELMIEDLMEQVVANGGSDLHISAGLPPYIRISGKLTPTDYEPLTPEQCQRLIFSMLNNTQRKHLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAACLRALSSKIPTFEQLGLPNIVREMSERPRGLILVTGPTGSGKTTTLAAMIDLINKTRAEHILTI
EDPIEFVYEPIKSLIHQRQVGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQQQIRVQLSNSLVAVFSQTLVPKKNPKPGEFGRIMAQEIMVVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYRAGIITYEAAMAKSSRQDELQRLIGAGAPAAAAR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=693808 NBE99_RS10205 WP_250681974.1 2122644..2123741(+) (pilT) [Thermosynechococcus sp. HN-54]
ATGGAGTTAATGATTGAAGACTTAATGGAGCAAGTGGTGGCCAATGGTGGCTCTGATTTGCACATCTCCGCTGGCTTACC
CCCCTACATTCGCATTAGCGGCAAATTAACGCCGACAGACTACGAGCCACTCACCCCAGAGCAATGCCAGCGCCTGATCT
TCAGCATGCTCAACAATACCCAACGTAAGCACCTAGAGCAAAACTGGGAGTTGGACTGCTCCTATGGTGTACGGGGTTTA
GCACGGTTCCGCGTCAATGTGTATAAAGACCGGGGTACCTATGCTGCTTGCTTGCGGGCTTTGAGTTCCAAAATTCCCAC
GTTTGAGCAGTTGGGATTGCCCAACATTGTCCGCGAAATGAGTGAGCGGCCACGGGGCTTGATTCTGGTGACTGGGCCTA
CAGGATCAGGGAAAACCACAACCTTGGCGGCGATGATTGACTTAATCAACAAAACCCGCGCTGAGCATATCCTGACGATT
GAAGACCCCATTGAGTTTGTCTATGAACCAATCAAGAGCCTGATCCACCAACGGCAGGTGGGGGAAGACACCAAGAGTTT
TGCCAATGCCCTACGGGCAGCGCTGCGGGAAGACCCCGACATTATCCTTGTGGGTGAGATGCGTGACTTGGAAACAATTC
AGCTGGCTATCTCAGCAGCGGAAACGGGTCACTTGGTCTTTGGGACGTTGCACACTAGTTCAGCCGCCCAAACCGTTGAC
CGTATGGTGGATGTGTTCCCGCCAGAGCAGCAACAGCAAATTCGCGTCCAGTTGTCGAACTCGTTGGTGGCAGTCTTTAG
CCAAACGCTGGTTCCTAAGAAAAATCCCAAGCCTGGTGAATTTGGGCGGATTATGGCCCAAGAAATCATGGTGGTGACGC
CTGCTATTTCCAACCTGATTCGCGAAGGCAAAACCTCGCAGATCTACTCGGCGATTCAGACCGGCGGCAAGCTGGGGATG
CAGACCCTCGAAAAAGTCTTGGCCGACTACTATCGTGCGGGGATTATTACCTATGAGGCAGCAATGGCCAAATCCTCGCG
TCAGGATGAGCTGCAACGTCTCATTGGTGCGGGTGCACCTGCGGCAGCAGCGCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.425

93.425

0.518
  pilT Legionella pneumophila strain ERS1305867

54.203

94.521

0.512
  pilT Legionella pneumophila strain Lp02

54.203

94.521

0.512
  pilT Pseudomonas stutzeri DSM 10701

53.936

93.973

0.507
  pilT Acinetobacter baylyi ADP1

54.572

92.877

0.507
  pilT Acinetobacter baumannii strain A118

53.412

92.329

0.493
  pilT Acinetobacter nosocomialis M2

53.412

92.329

0.493
  pilT Acinetobacter baumannii D1279779

53.412

92.329

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.286

95.89

0.482
  pilT Neisseria gonorrhoeae MS11

49.284

95.616

0.471
  pilT Neisseria meningitidis 8013

49.284

95.616

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

93.151

0.466
  pilT Vibrio cholerae strain A1552

50

93.151

0.466
  pilU Pseudomonas stutzeri DSM 10701

39.155

97.26

0.381
  pilU Acinetobacter baylyi ADP1

38.109

95.616

0.364