Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NBE99_RS10200 Genome accession   NZ_CP098039
Coordinates   2120546..2122576 (+) Length   676 a.a.
NCBI ID   WP_250681973.1    Uniprot ID   -
Organism   Thermosynechococcus sp. HN-54     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2115546..2127576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBE99_RS10175 (NBE99_10175) - 2116201..2117211 (+) 1011 WP_250681969.1 hypothetical protein -
  NBE99_RS10180 (NBE99_10180) - 2117208..2118812 (-) 1605 WP_250681970.1 right-handed parallel beta-helix repeat-containing protein -
  NBE99_RS10185 (NBE99_10185) - 2118779..2118973 (-) 195 WP_250681971.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  NBE99_RS10190 (NBE99_10190) - 2118974..2119186 (-) 213 WP_250681972.1 hypothetical protein -
  NBE99_RS10195 (NBE99_10195) - 2119493..2120455 (+) 963 WP_399371026.1 transposase -
  NBE99_RS10200 (NBE99_10200) pilB/pilB1 2120546..2122576 (+) 2031 WP_250681973.1 GspE/PulE family protein Machinery gene
  NBE99_RS10205 (NBE99_10205) pilT 2122644..2123741 (+) 1098 WP_250681974.1 type IV pilus twitching motility protein PilT Machinery gene
  NBE99_RS10210 (NBE99_10210) - 2123791..2125011 (+) 1221 WP_250681975.1 type II secretion system F family protein -
  NBE99_RS10215 (NBE99_10215) - 2125025..2125498 (+) 474 WP_250681976.1 J domain-containing protein -
  NBE99_RS10220 (NBE99_10220) - 2125495..2125767 (+) 273 WP_250681977.1 DUF3143 domain-containing protein -
  NBE99_RS10225 (NBE99_10225) - 2125775..2126113 (-) 339 WP_250681978.1 hypothetical protein -
  NBE99_RS10230 (NBE99_10230) - 2126420..2127571 (-) 1152 WP_250681979.1 cysteine desulfurase family protein -

Sequence

Protein


Download         Length: 676 a.a.        Molecular weight: 74483.75 Da        Isoelectric Point: 5.6908

>NTDB_id=693807 NBE99_RS10200 WP_250681973.1 2120546..2122576(+) (pilB/pilB1) [Thermosynechococcus sp. HN-54]
MVNTSSSSSSARKALTVRGRAASPTERAIVASGYASIDQVREAMNTARKTGKSLVTVLQEITGTTMPPDVLRQYHKQQLF
ELKVIYGVDCLDPELNRFPTEQIEQLINTIVPIDTCRTYQVIPIAKHLDADPPYLLVAMVDPDNLQAIDNLTRLLRSHNL
TLKRMVITLEDYQRLIDPILNKQVAETAASKNAPAAIGEINIEEDIEAMGGLEEIEGEQEADLAEALKGAEDAPIIALVN
KILAKALTEGVSDIHIEPQEEYLRIRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRV
DFRVNTLPSRWGEKVVLRILDNSATQLGLDKLITDPESLAIVREMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVN
ISTAEDPIEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAV
ARLSEMGVEPFMVSASLIGVVAQRLMRRVCSECRIPYTPTREELARFGLSASKDVNLTLYKANKLTPEQIQAAKASGQPI
CSKCGGVGYKGRCGVYEIMRVTERLQSLITEGAPTERIKEAAVEEGMKTLLAYSLNLVKEGVTTLEEVERVTFTDTGLES
ELKAKRKSSLTCRTCGAEAQPEWLECPYCMTPRFVD

Nucleotide


Download         Length: 2031 bp        

>NTDB_id=693807 NBE99_RS10200 WP_250681973.1 2120546..2122576(+) (pilB/pilB1) [Thermosynechococcus sp. HN-54]
ATGGTTAACACATCGTCATCTTCTTCATCCGCTCGCAAAGCGCTTACCGTGCGCGGCAGAGCCGCTAGCCCCACCGAACG
AGCCATTGTTGCCTCCGGTTACGCCAGTATCGATCAGGTGCGGGAAGCCATGAACACCGCCCGCAAAACAGGCAAGTCCC
TCGTAACCGTGTTGCAGGAGATTACGGGCACCACCATGCCCCCCGATGTGTTACGTCAGTACCACAAGCAACAACTTTTT
GAACTTAAAGTCATCTATGGTGTCGATTGCCTTGATCCCGAACTCAATCGTTTTCCCACCGAGCAAATCGAGCAACTGAT
CAACACGATTGTACCGATTGACACCTGCCGCACCTACCAAGTCATCCCCATTGCCAAGCACCTCGACGCCGATCCCCCCT
ACCTGCTGGTGGCGATGGTGGATCCCGACAACCTGCAAGCCATTGACAACCTCACCCGGCTGCTGCGCAGTCACAACCTC
ACCCTGAAGCGGATGGTGATCACCCTAGAGGACTACCAACGCCTCATTGACCCGATTCTCAACAAGCAGGTAGCTGAAAC
CGCTGCCAGCAAAAATGCCCCCGCTGCTATCGGTGAAATCAACATCGAAGAAGACATCGAAGCCATGGGCGGCCTCGAAG
AAATTGAAGGGGAGCAGGAGGCTGACCTTGCCGAGGCGCTCAAAGGGGCAGAAGATGCTCCCATTATTGCGCTTGTCAAC
AAAATTTTGGCCAAGGCTCTCACCGAAGGAGTTTCCGACATCCACATTGAACCCCAAGAGGAATACCTACGGATTCGTTT
CCGTAAAGATGGGGTGCTCCACCAGGCCTTTGACCCCCTGCCGAAGAAGATCGTGCCAGCCGTAGTTTCCCGCTTCAAAA
TTTTGGCTGACCTCGACATTGCCGAGCGGCGTGCCCCCCAAGATGGCCGTATCCGCAAAATGTTCCAAGGGCGACGGGTG
GACTTCCGAGTGAATACCCTCCCCAGCCGCTGGGGCGAAAAAGTGGTACTGCGGATTCTCGATAACTCCGCCACCCAATT
GGGTCTCGACAAACTGATTACTGACCCTGAGAGCCTTGCCATTGTGCGGGAGATGACCAAGCGCCCCTTTGGTTTGATTC
TGGTCACAGGCCCGACGGGTTCAGGGAAAACCACCACACTTTACTCAGCGCTGGCTGAATGTAATAGTCCTGGTGTCAAC
ATCAGTACAGCGGAAGACCCAATTGAATACACGCTCCCCGGCCTGACGCAGGTACAGGTTATTCGCGAAAAAGGTATGGA
CTTTGCCTCCATTCTGCGTGCCTTTCTCCGCCAAGACCCCGATGTGATTCTGGTGGGGGAAACCCGCGACAAGGAAACAG
CAAAAACCGCTATTGAAGCTGCCTTGACGGGTCACTTGGTGTTAACCACTCTGCACACCAACGATGCCGCCAGTGCCGTT
GCCCGTCTCTCGGAAATGGGGGTGGAACCCTTCATGGTCTCCGCTTCCTTAATTGGGGTCGTTGCCCAGCGACTCATGCG
GCGGGTATGCAGTGAGTGTCGCATTCCCTATACCCCCACCCGTGAGGAACTGGCACGCTTTGGCTTGTCCGCTTCCAAGG
ATGTCAACCTCACCCTCTACAAAGCCAATAAACTGACGCCCGAGCAGATTCAAGCAGCCAAAGCCAGTGGCCAGCCGATT
TGCAGCAAGTGTGGCGGTGTGGGCTACAAAGGGCGCTGTGGGGTCTATGAAATTATGCGGGTGACGGAGCGCCTGCAAAG
TCTGATTACTGAGGGTGCCCCCACGGAGCGGATCAAAGAAGCGGCGGTTGAAGAGGGCATGAAAACCCTGTTGGCCTATA
GCCTGAACCTTGTCAAAGAAGGCGTCACCACCCTCGAAGAAGTCGAGCGCGTCACCTTTACGGACACGGGTCTCGAATCA
GAACTCAAGGCCAAACGTAAGAGTTCTTTAACCTGCCGTACCTGCGGTGCCGAGGCCCAGCCCGAATGGTTAGAATGCCC
CTATTGCATGACACCCCGCTTTGTGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(76-180)

Type II/IV secretion system protein (T2SSE)

(241-508)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.244

97.041

0.633