Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NAL25_RS21255 Genome accession   NZ_CP098030
Coordinates   4451382..4452404 (-) Length   340 a.a.
NCBI ID   WP_257711414.1    Uniprot ID   -
Organism   Serratia ureilytica strain HNU47     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4446382..4457404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAL25_RS21225 (NAL25_21200) - 4447181..4447693 (+) 513 WP_046897323.1 SprT family zinc-dependent metalloprotease -
  NAL25_RS21230 (NAL25_21205) endA 4447795..4448490 (+) 696 WP_025304243.1 deoxyribonuclease I -
  NAL25_RS21235 (NAL25_21210) rsmE 4448560..4449291 (+) 732 WP_257711412.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NAL25_RS21240 (NAL25_21215) gshB 4449302..4450252 (+) 951 WP_046897322.1 glutathione synthase -
  NAL25_RS21245 (NAL25_21220) - 4450400..4450963 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  NAL25_RS21250 (NAL25_21225) ruvX 4450963..4451385 (+) 423 WP_257711413.1 Holliday junction resolvase RuvX -
  NAL25_RS21255 (NAL25_21230) pilT 4451382..4452404 (-) 1023 WP_257711414.1 type IV pilus twitching motility protein PilT Machinery gene
  NAL25_RS21260 (NAL25_21235) - 4452425..4453132 (+) 708 WP_004937443.1 YggS family pyridoxal phosphate-dependent enzyme -
  NAL25_RS21265 (NAL25_21240) proC 4453152..4453973 (+) 822 WP_257711415.1 pyrroline-5-carboxylate reductase -
  NAL25_RS21270 (NAL25_21245) - 4454005..4454559 (+) 555 WP_257711416.1 YggT family protein -
  NAL25_RS21275 (NAL25_21250) yggU 4454556..4454846 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  NAL25_RS21280 (NAL25_21255) - 4454864..4455457 (+) 594 WP_193550277.1 XTP/dITP diphosphatase -
  NAL25_RS21285 (NAL25_21260) hemW 4455450..4456592 (+) 1143 WP_193550278.1 radical SAM family heme chaperone HemW -
  NAL25_RS21290 (NAL25_21265) - 4456629..4456712 (-) 84 Protein_4163 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36329.63 Da        Isoelectric Point: 7.5735

>NTDB_id=693652 NAL25_RS21255 WP_257711414.1 4451382..4452404(-) (pilT) [Serratia ureilytica strain HNU47]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCNGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTAAAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=693652 NAL25_RS21255 WP_257711414.1 4451382..4452404(-) (pilT) [Serratia ureilytica strain HNU47]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTTTGCAACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCTTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGTGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAGGATCCGGATGTGATCCTGTTGGGTGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGACGCCCCGGCGGCGGACGAGTGGCGATCTTTGAAGTGCTGACGGCGGCGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Neisseria meningitidis 8013

50.755

97.353

0.494
  pilT Neisseria gonorrhoeae MS11

50.453

97.353

0.491
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.029

100

0.453
  pilU Vibrio cholerae strain A1552

40.184

95.882

0.385
  pilU Acinetobacter baylyi ADP1

39.628

95

0.376
  pilU Pseudomonas stutzeri DSM 10701

38.509

94.706

0.365