Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NAG75_RS03400 Genome accession   NZ_CP097873
Coordinates   539938..541044 (+) Length   368 a.a.
NCBI ID   WP_065764212.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain GL-601     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 534938..546044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAG75_RS03370 - 535391..535738 (+) 348 WP_031430599.1 hypothetical protein -
  NAG75_RS03375 yggU 536284..536577 (-) 294 WP_031430601.1 DUF167 family protein YggU -
  NAG75_RS03380 - 536577..537134 (-) 558 WP_005482440.1 YggT family protein -
  NAG75_RS03385 proC 537187..538005 (-) 819 WP_005482485.1 pyrroline-5-carboxylate reductase -
  NAG75_RS03390 - 538125..538838 (-) 714 WP_021450047.1 YggS family pyridoxal phosphate-dependent enzyme -
  NAG75_RS03395 pilT 538867..539907 (+) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  NAG75_RS03400 pilU 539938..541044 (+) 1107 WP_065764212.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NAG75_RS03405 ruvX 541106..541531 (-) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  NAG75_RS03410 - 541580..542143 (-) 564 WP_005461717.1 YqgE/AlgH family protein -
  NAG75_RS03415 gshB 542257..543207 (-) 951 WP_005425823.1 glutathione synthase -
  NAG75_RS03420 rsmE 543222..543953 (-) 732 WP_005461719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NAG75_RS03425 endA 544078..544773 (-) 696 WP_005493878.1 deoxyribonuclease I -
  NAG75_RS03430 - 544893..545390 (-) 498 WP_015297290.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41443.67 Da        Isoelectric Point: 7.0235

>NTDB_id=693193 NAG75_RS03400 WP_065764212.1 539938..541044(+) (pilU) [Vibrio parahaemolyticus strain GL-601]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAKLLDSAMDPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETIIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPRDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=693193 NAG75_RS03400 WP_065764212.1 539938..541044(+) (pilU) [Vibrio parahaemolyticus strain GL-601]
ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAGAAACTCACCGAACATGATGTTGCAAAGTTGCTTGATAGCGCAA
TGGATCCGGATCGGCGTCAGGAGTTTCGCAAAAGCCGCGAATCAAACTTTGCTATCGTCAGAGATTGTGGTCGCTTCCGT
GTTAGTGCTTTTTTTCAGCGAGAACTTCCGGGAGCAGTCATTCGCCGTATCGAAACCATTATTCCTACTTTTGAGCAGTT
AAAATTGCCTTTAGTGCTGCAAGACTTAGCGATTGCCAAGCGTGGTTTGGTGCTGGTGGTCGGCGCTACTGGCTCGGGTA
AATCGACCACGATGGCGGCGATGACGGGCTATCGAAACAGCAACAAAACCGGTCACATCTTGACGGTCGAAGATCCGATC
GAATTTGTGCATGAGCATAAGCGTTGTATCGTGACTCAGCGTGAGGTGGGCTTGGATACCGAAAGCTATGAAGTCGCACT
GAAAAACTCGTTGCGCCAAGCGCCTGATATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACTTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCGTTAGAACGTATTCTCCAT
TTGGTGCCTAGAGATCAAAAAGAGCAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGCGTGATTGGTCAGCAGCTGAT
CCGTGATAAGAATGGTCAAGGGCGTCATGGTGTGTTTGAAATCTTGCTAAACAGTCCACGAGTGTCGGATTTGATCCGTC
GTGGTGATTTACATGAATTGAAGTCAACCATGGCACGATCCAATGAGTTTGGCATGCTGACGTTTGACCAATCGCTCTAC
AAGCTGGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTGCACAGCGCGGATTCTGCCAACGATCTTCGTTTAATGCT
GAAAACACAGCGTGGTGAGCCGTTCTCTACAGGCAGTCTTGCCAACGTCAAAATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

55.271

95.38

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

92.12

0.405
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Acinetobacter nosocomialis M2

42.478

92.12

0.391
  pilT Acinetobacter baumannii D1279779

42.478

92.12

0.391
  pilT Acinetobacter baumannii strain A118

42.478

92.12

0.391
  pilT Pseudomonas aeruginosa PAK

42.183

92.12

0.389
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

41.994

89.946

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.361

90.217

0.364
  pilT Neisseria meningitidis 8013

40.361

90.217

0.364