Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M9O80_RS03685 Genome accession   NZ_CP097808
Coordinates   745395..746375 (+) Length   326 a.a.
NCBI ID   WP_048268202.1    Uniprot ID   -
Organism   Klebsiella variicola strain 17YN56     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 740395..751375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9O80_RS03650 (M9O80_03650) - 740931..741257 (+) 327 WP_012967224.1 YggL family protein -
  M9O80_RS03655 (M9O80_03655) - 741308..742027 (+) 720 WP_012540560.1 DUF2884 domain-containing protein -
  M9O80_RS03660 (M9O80_03660) hemW 742068..743204 (-) 1137 WP_002916617.1 radical SAM family heme chaperone HemW -
  M9O80_RS03665 (M9O80_03665) - 743197..743790 (-) 594 WP_012540562.1 XTP/dITP diphosphatase -
  M9O80_RS03670 (M9O80_03670) yggU 743803..744093 (-) 291 WP_004205183.1 DUF167 family protein YggU -
  M9O80_RS03675 (M9O80_03675) - 744090..744656 (-) 567 WP_004205182.1 YggT family protein -
  M9O80_RS03680 (M9O80_03680) - 744677..745378 (-) 702 WP_008806467.1 YggS family pyridoxal phosphate-dependent enzyme -
  M9O80_RS03685 (M9O80_03685) pilT 745395..746375 (+) 981 WP_048268202.1 type IV pilus twitching motility protein PilT Machinery gene
  M9O80_RS03690 (M9O80_03690) - 746724..747377 (+) 654 WP_004205179.1 LuxR family transcriptional regulator -
  M9O80_RS03695 (M9O80_03695) ruvX 747430..747846 (-) 417 WP_012540565.1 Holliday junction resolvase RuvX -
  M9O80_RS03700 (M9O80_03700) - 747846..748409 (-) 564 WP_008806463.1 YqgE/AlgH family protein -
  M9O80_RS03705 (M9O80_03705) gshB 748583..749533 (-) 951 WP_008806462.1 glutathione synthase -
  M9O80_RS03710 (M9O80_03710) rsmE 749554..750285 (-) 732 WP_008806461.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M9O80_RS03715 (M9O80_03715) endA 750377..751084 (-) 708 WP_008806460.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35407.92 Da        Isoelectric Point: 6.8129

>NTDB_id=692752 M9O80_RS03685 WP_048268202.1 745395..746375(+) (pilT) [Klebsiella variicola strain 17YN56]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLEPAPFPAPDIPDLLNCWLDAAQLQHWQEHGQIDFALTLACGSRLRA
SAFAHTRGISLVLRLLPEQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQRLDGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGEL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=692752 M9O80_RS03685 WP_048268202.1 745395..746375(+) (pilT) [Klebsiella variicola strain 17YN56]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGCCCGCCCCGTTTCCCGCGCCTGATATCCCGGATTTACTCAACTGCTGGCTTGATG
CCGCACAGTTACAGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTTCGCGGCTGCGCGCC
AGCGCTTTCGCCCATACGCGTGGAATATCGCTGGTGCTACGCCTTCTGCCTGAACAGTGCCCGCGTCTGGAGACGCTGGG
CGCCCCGCCTGCGCTGAGCGAGCTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCGATGGTGGGGCATCTCAACCAGCGTCTTGATGGCCATATTCTGACCCTGGAGGACCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCAGCAGCGGGAGATTGGCCGTCATTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGACGTGATCCTGCTCGGGGAGTTACGCGACAGCGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAGACCGGACATCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGTAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCGCGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTTTGGTCAATACCCCCGCAGTAGCGAATTTGATCCGTGAAGGAA
AAGTGCACCAGTTGCCCGGCGTGATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACGCAGAGTTTTCAGCAACGT
GTCGCCGCAGGCGAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.376

100

0.515
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.376

100

0.515
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

47.401

100

0.475
  pilT Legionella pneumophila strain ERS1305867

47.531

99.387

0.472
  pilT Legionella pneumophila strain Lp02

47.531

99.387

0.472
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.164

97.239

0.429
  pilU Vibrio cholerae strain A1552

40.122

100

0.405
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.405
  pilU Acinetobacter baylyi ADP1

38.941

98.466

0.383