Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M8232_RS03535 Genome accession   NZ_CP097646
Coordinates   717013..717993 (+) Length   326 a.a.
NCBI ID   WP_017899769.1    Uniprot ID   -
Organism   Klebsiella quasipneumoniae strain NE1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 712013..722993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8232_RS03505 (M8232_03505) - 712395..713381 (-) 987 WP_053809958.1 TRAP transporter substrate-binding protein -
  M8232_RS03510 (M8232_03510) hemW 713686..714822 (-) 1137 WP_064183218.1 radical SAM family heme chaperone HemW -
  M8232_RS03515 (M8232_03515) - 714815..715408 (-) 594 WP_004205185.1 XTP/dITP diphosphatase -
  M8232_RS03520 (M8232_03520) yggU 715421..715711 (-) 291 WP_023319134.1 DUF167 family protein YggU -
  M8232_RS03525 (M8232_03525) - 715708..716274 (-) 567 WP_004205182.1 YggT family protein -
  M8232_RS03530 (M8232_03530) - 716295..716996 (-) 702 WP_004205181.1 YggS family pyridoxal phosphate-dependent enzyme -
  M8232_RS03535 (M8232_03535) pilT 717013..717993 (+) 981 WP_017899769.1 type IV pilus twitching motility protein PilT Machinery gene
  M8232_RS03540 (M8232_03540) - 718342..718995 (+) 654 WP_156234289.1 LuxR C-terminal-related transcriptional regulator -
  M8232_RS03545 (M8232_03545) ruvX 719016..719432 (-) 417 WP_156234287.1 Holliday junction resolvase RuvX -
  M8232_RS03550 (M8232_03550) - 719432..719995 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  M8232_RS03555 (M8232_03555) gshB 720170..721120 (-) 951 WP_004205176.1 glutathione synthase -
  M8232_RS03560 (M8232_03560) rsmE 721141..721872 (-) 732 WP_156234285.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M8232_RS03565 (M8232_03565) endA 721964..722671 (-) 708 WP_004205174.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35304.69 Da        Isoelectric Point: 6.7313

>NTDB_id=691428 M8232_RS03535 WP_017899769.1 717013..717993(+) (pilT) [Klebsiella quasipneumoniae strain NE1]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLEPAPFPAPDIADLLNDWLDAAQLQHWQEHGQIDFALTLACGSRLRA
SAFAHTRGISLVLRLLPSQCPRLEALGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFSAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVGNLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGEL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=691428 M8232_RS03535 WP_017899769.1 717013..717993(+) (pilT) [Klebsiella quasipneumoniae strain NE1]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGTAAGCTGGAGCCCGCGCCCTTTCCCGCACCGGATATTGCGGATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACAGCACTGGCAGGAGCACGGCCAGATAGACTTTGCGCTGACTCTGGCCTGTGGTTCACGGTTACGCGCC
AGCGCTTTCGCCCATACGCGCGGAATATCGCTGGTGCTGCGCCTGCTGCCCAGCCAGTGTCCGCGCCTGGAGGCGCTGGG
CGCGCCGCCTGCGCTAAGCGAGCTGCTGGCGGAAGAGAGTGGTTTGCTGCTGGTCACCGGGGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCGATGGTCGGCCATCTCAACCAGCATCTTGATGGCCATATTCTGACGCTGGAGGATCCGGTGGAG
TTTATTCATCACAGCGAGCGATGCCTGATTCAGCAGCGGGAGATTGGCCGGCACTGTCCTTCGTTCTCCGCAGCGCTTCG
CGTGGCGCTGCGTCAGGATCCGGATGTGATCCTGCTCGGCGAGCTACGCGACAGCGAAACCATTCGTCTGGCGTTAACTG
CGGCGGAGACCGGGCATCTGGTGATGGCGACATTACACACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGTAGCCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTATTACC
CGCACGTCAGGGCGGCAGAGTTGCGCTATACGAGCTTCTGGTTAATACGCCGGCGGTAGGGAATTTGATCCGCGAAGGAA
AAGTGCATCAGCTGCCCGGTGTAATGCAGACCGGGATGCAGGCCGGAATGTTGACATTTACCCAGAGTTTTCAGCAACGT
GTGGCCGCAGGCGAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.682

100

0.518
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.682

100

0.518
  pilT Acinetobacter baylyi ADP1

48.93

100

0.491
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Legionella pneumophila strain ERS1305867

48.148

99.387

0.479
  pilT Legionella pneumophila strain Lp02

48.148

99.387

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.164

97.239

0.429
  pilU Vibrio cholerae strain A1552

40.122

100

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Acinetobacter baylyi ADP1

39.564

98.466

0.39