Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M8333_RS04165 Genome accession   NZ_CP097554
Coordinates   855441..856421 (+) Length   326 a.a.
NCBI ID   WP_025105940.1    Uniprot ID   A0AB35W6R2
Organism   Klebsiella michiganensis strain YZUMF202001     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 850441..861421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8333_RS04135 (M8333_04140) - 850823..851809 (-) 987 WP_014839537.1 TRAP transporter substrate-binding protein -
  M8333_RS04140 (M8333_04145) hemW 852114..853250 (-) 1137 WP_014226840.1 radical SAM family heme chaperone HemW -
  M8333_RS04145 (M8333_04150) rdgB 853243..853836 (-) 594 WP_224266128.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  M8333_RS04150 (M8333_04155) yggU 853849..854139 (-) 291 WP_004854155.1 DUF167 family protein YggU -
  M8333_RS04155 (M8333_04160) - 854136..854702 (-) 567 WP_004105714.1 YggT family protein -
  M8333_RS04160 (M8333_04165) - 854720..855424 (-) 705 WP_025105939.1 YggS family pyridoxal phosphate-dependent enzyme -
  M8333_RS04165 (M8333_04170) pilT 855441..856421 (+) 981 WP_025105940.1 type IV pilus twitching motility protein PilT Machinery gene
  M8333_RS04170 (M8333_04175) - 856770..857423 (+) 654 WP_014226837.1 LuxR C-terminal-related transcriptional regulator -
  M8333_RS04175 (M8333_04180) ruvX 857479..857895 (-) 417 WP_014226836.1 Holliday junction resolvase RuvX -
  M8333_RS04180 (M8333_04185) - 857895..858458 (-) 564 WP_014226835.1 YqgE/AlgH family protein -
  M8333_RS04185 (M8333_04190) gshB 858635..859582 (-) 948 WP_014226834.1 glutathione synthase -
  M8333_RS04190 (M8333_04195) rsmE 859603..860334 (-) 732 WP_032720268.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M8333_RS04195 (M8333_04200) endA 860426..861133 (-) 708 WP_014226832.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35219.43 Da        Isoelectric Point: 6.8188

>NTDB_id=689980 M8333_RS04165 WP_025105940.1 855441..856421(+) (pilT) [Klebsiella michiganensis strain YZUMF202001]
MELEEIVALSVKHNVSDLHLCNASTPRWRRQGKLEPAPFTSPDIGKILADWLNAEQLAQWQACGQVDFALAPAGGPRLRA
SAFVHNRGLSLALRLLAETCPQLADLGAPAALTELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLNGHILTLEDPVE
FIHRSERCLIQQREIGRHCPSFAAALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAHEKEQVRSQLAGSLCAVLAQKLFPDTAGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQFGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=689980 M8333_RS04165 WP_025105940.1 855441..856421(+) (pilT) [Klebsiella michiganensis strain YZUMF202001]
ATGGAACTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCTTCAACGCCGCG
CTGGCGTCGCCAGGGCAAGCTGGAGCCCGCGCCGTTTACCTCTCCGGATATTGGCAAGATACTCGCTGATTGGCTCAACG
CCGAGCAGCTCGCCCAGTGGCAGGCCTGCGGACAGGTCGATTTCGCGCTGGCTCCGGCCGGCGGACCGCGCCTGCGCGCC
AGCGCATTTGTCCACAACCGCGGTCTGTCGCTGGCCCTGCGTCTGCTGGCGGAAACCTGTCCGCAGCTGGCGGATCTCGG
CGCGCCGGCCGCCTTAACGGAGCTGCTCAATGAGGAGAGCGGGCTTATCCTGGTCACGGGCGCCACCGGTAGCGGGAAAT
CCACCACCCTGGCGGCGATGGTGGGCTATCTTAATCAGCGCCTCAATGGCCATATCCTGACGCTGGAAGATCCGGTTGAG
TTTATTCACCGCAGCGAGCGCTGCCTGATCCAGCAGCGGGAAATAGGTCGCCACTGCCCCTCATTTGCCGCGGCCCTGCG
CGCTGCGCTGCGTCAGGACCCGGATGTCATTCTGCTAGGGGAACTGCGTGACAGCGAAACGATCCGCCTGGCACTGACCG
CGGCGGAGACCGGACATCTGGTTATGGCGACGCTGCATACGCGCGGCGCGGCACAGGCGGTTGAAAGGCTGGTGGATGTT
TTTCCTGCCCATGAGAAAGAGCAGGTTCGTAGCCAGCTGGCGGGAAGTTTATGCGCGGTGCTGGCACAAAAATTGTTTCC
GGATACTGCTGGCGGAAGAGTTGCGCTATACGAGCTTTTGGTTAATACCCCGGCGGTGGCGAATTTGATCCGCGAGGGGA
AAACGCATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAATTCGGGATGCAAACTTTTACGCAAAGCTTTCAGCAACGT
ACGGCTGCGGGTCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.848

100

0.503
  pilT Vibrio cholerae strain A1552

49.848

100

0.503
  pilT Neisseria meningitidis 8013

48.182

100

0.488
  pilT Neisseria gonorrhoeae MS11

47.879

100

0.485
  pilT Legionella pneumophila strain Lp02

48.113

97.546

0.469
  pilT Legionella pneumophila strain ERS1305867

48.113

97.546

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.201

100

0.466
  pilT Acinetobacter baumannii strain A118

45.455

100

0.46
  pilT Acinetobacter baumannii D1279779

45.455

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.152

100

0.457
  pilT Acinetobacter baylyi ADP1

44.985

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.414
  pilU Vibrio cholerae strain A1552

40.683

98.773

0.402
  pilU Acinetobacter baylyi ADP1

38.39

99.08

0.38