Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   UtA1_RS08770 Genome accession   NZ_AP018335
Coordinates   1829058..1830101 (+) Length   347 a.a.
NCBI ID   WP_096550733.1    Uniprot ID   -
Organism   Ureibacillus thermosphaericus strain A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1824058..1835101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UtA1_RS08750 - 1824292..1824771 (-) 480 WP_096550729.1 hypothetical protein -
  UtA1_RS08755 - 1825080..1825535 (-) 456 WP_096550730.1 hypothetical protein -
  UtA1_RS08760 - 1826027..1827361 (+) 1335 WP_096550731.1 VanW family protein -
  UtA1_RS08765 - 1827377..1829044 (+) 1668 WP_096550732.1 GspE/PulE family protein -
  UtA1_RS08770 pilT 1829058..1830101 (+) 1044 WP_096550733.1 type IV pilus twitching motility protein PilT Machinery gene
  UtA1_RS08775 - 1830098..1831306 (+) 1209 WP_096550734.1 type II secretion system F family protein -
  UtA1_RS08780 - 1831419..1831889 (+) 471 WP_096550735.1 type II secretion system protein -
  UtA1_RS08785 pilM 1832006..1832995 (+) 990 WP_096550736.1 type IV pilus biogenesis protein PilM -
  UtA1_RS08790 - 1832979..1833566 (+) 588 WP_096550737.1 hypothetical protein -
  UtA1_RS08795 - 1833563..1834252 (+) 690 WP_096550738.1 potassium transporter -
  UtA1_RS08800 - 1834281..1835036 (+) 756 WP_096550739.1 A24 family peptidase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38836.28 Da        Isoelectric Point: 6.5591

>NTDB_id=68941 UtA1_RS08770 WP_096550733.1 1829058..1830101(+) (pilT) [Ureibacillus thermosphaericus strain A1]
MGYSIQDLLQKTYELNASDLHITVGTPPVYRIHGQLERFGDSELIAEEIIDMVNAIIPDNKLEEYRSKGDADFNYAFNEN
FRFRVNAYHQRNAPALAFRLIPTKIPTIESLGMPKVLYDLADKPQGLVLVTGPTGSGKSSTLAAMIHHINETKAKHIITL
EDPIEFIHQHNQSIVNQREIGIDAKDFATGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVFGTLHTSSAPTTID
RIIDVFPPHQQNQIRNQLANVLQGVISQRLFVRKDRSGRVAATEILVAVPSVTNLIRNEKIHQIQNIMQTSRALGMHTLQ
HSIQELLSRGIVSFEEVKPYMNVGDYH

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=68941 UtA1_RS08770 WP_096550733.1 1829058..1830101(+) (pilT) [Ureibacillus thermosphaericus strain A1]
ATGGGATATTCAATCCAAGATTTACTACAAAAAACTTATGAATTAAATGCTTCAGATTTACACATTACCGTTGGAACTCC
ACCAGTGTACCGAATTCACGGTCAATTAGAAAGGTTTGGAGATTCCGAGTTGATTGCTGAAGAGATTATTGACATGGTGA
ACGCTATTATTCCGGATAACAAATTAGAAGAATATCGTTCAAAAGGAGATGCAGATTTTAATTACGCTTTTAACGAAAAT
TTTCGATTCCGTGTCAATGCTTATCATCAACGAAATGCACCAGCATTAGCTTTTCGATTAATTCCAACGAAAATCCCGAC
AATTGAATCGCTCGGAATGCCGAAAGTGTTATATGATTTGGCGGATAAACCACAAGGACTTGTGCTAGTAACGGGGCCTA
CAGGTTCTGGTAAATCTTCTACGTTGGCAGCAATGATTCACCATATCAACGAAACGAAGGCAAAACACATTATTACGCTA
GAAGATCCGATTGAATTTATTCATCAACATAATCAATCCATTGTCAATCAACGGGAAATTGGAATTGATGCAAAAGATTT
TGCAACAGGACTAAGAGCTGCTCTTCGTCAAGACCCAGATGTCATTCTCGTTGGAGAAATGCGGGATTTAGAAACGATAT
CAACTGCAATTACTGCTGCAGAAACAGGGCATTTAGTGTTTGGTACACTTCATACATCCAGTGCACCGACAACGATTGAT
CGTATTATCGATGTTTTTCCACCACATCAGCAAAATCAAATTCGCAATCAACTTGCTAATGTGTTGCAAGGAGTAATTTC
CCAACGACTTTTCGTTCGAAAAGATAGAAGTGGCCGTGTAGCAGCAACCGAAATTCTTGTGGCTGTGCCGTCAGTGACAA
ATTTAATTCGCAACGAAAAGATTCACCAAATTCAAAATATCATGCAAACGAGCCGTGCACTAGGCATGCATACGTTGCAA
CATTCAATTCAAGAGTTGCTTTCAAGAGGCATCGTGTCGTTTGAGGAAGTGAAACCTTACATGAACGTTGGTGATTACCA
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.851

96.542

0.481
  pilT Neisseria meningitidis 8013

50.151

95.389

0.478
  pilT Vibrio cholerae strain A1552

49.401

96.254

0.476
  pilT Neisseria gonorrhoeae MS11

49.401

96.254

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.401

96.254

0.476
  pilT Acinetobacter nosocomialis M2

49.24

94.813

0.467
  pilT Acinetobacter baumannii D1279779

48.936

94.813

0.464
  pilT Acinetobacter baumannii strain A118

48.936

94.813

0.464
  pilT Pseudomonas stutzeri DSM 10701

47.605

96.254

0.458
  pilT Pseudomonas aeruginosa PAK

47.605

96.254

0.458
  pilT Acinetobacter baylyi ADP1

48.182

95.101

0.458
  pilT Legionella pneumophila strain Lp02

48.024

94.813

0.455
  pilT Legionella pneumophila strain ERS1305867

48.024

94.813

0.455
  pilU Pseudomonas stutzeri DSM 10701

40.361

95.677

0.386
  pilU Acinetobacter baylyi ADP1

39.759

95.677

0.38
  pilU Vibrio cholerae strain A1552

41.346

89.914

0.372

Multiple sequence alignment