Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CDC37_RS14580 Genome accession   NZ_AP018254
Coordinates   3612363..3613484 (+) Length   373 a.a.
NCBI ID   WP_096622667.1    Uniprot ID   A0A1Z4NKS4
Organism   Calothrix sp. NIES-3974     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3607363..3618484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDC37_RS24390 - 3607654..3607809 (+) 156 WP_157780757.1 hypothetical protein -
  CDC37_RS14565 (NIES3974_29770) - 3607802..3608581 (+) 780 WP_096622664.1 hypothetical protein -
  CDC37_RS14570 (NIES3974_29780) - 3608621..3609763 (-) 1143 WP_096622665.1 quinone-dependent dihydroorotate dehydrogenase -
  CDC37_RS14575 (NIES3974_29790) pilB/pilB1 3610074..3612086 (+) 2013 WP_096622666.1 GspE/PulE family protein Machinery gene
  CDC37_RS14580 (NIES3974_29800) pilT 3612363..3613484 (+) 1122 WP_096622667.1 type IV pilus twitching motility protein PilT Machinery gene
  CDC37_RS14585 (NIES3974_29810) - 3613574..3614788 (+) 1215 WP_096622668.1 type II secretion system F family protein -
  CDC37_RS14590 (NIES3974_29820) - 3614938..3615471 (+) 534 WP_096626235.1 hypothetical protein -
  CDC37_RS14595 (NIES3974_29830) - 3615486..3616031 (-) 546 WP_096622669.1 hypothetical protein -
  CDC37_RS14600 (NIES3974_29840) hisA 3616252..3617025 (-) 774 WP_096626237.1 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase -
  CDC37_RS14605 (NIES3974_29850) - 3617042..3617386 (-) 345 WP_096622670.1 DUF3593 domain-containing protein -
  CDC37_RS14610 (NIES3974_29860) - 3617579..3618454 (-) 876 WP_096622671.1 S-methyl-5'-thioadenosine phosphorylase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41048.28 Da        Isoelectric Point: 7.3995

>NTDB_id=68915 CDC37_RS14580 WP_096622667.1 3612363..3613484(+) (pilT) [Calothrix sp. NIES-3974]
MDYMIEDLMEDMIEQGGSDMHLSAGLPPYFRVSGKLTPQDKYGVLSVDNCQRLIFSMLNNSQRKTLEQTWELDCSYGVKG
LARFRVNVYKDRGAYAACLRALSSKIPNFDKLGLPDVVREMSEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILT
VEDPIEFVYEPIKSLIHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTV
DRIIDVFPTERQQQVRVQLSNSLVAVFSQTLVPRKNPKPGEFGRVMAQEIMIVTPAISNLIREGKTAQIYSAIQTGGKLG
MQTLEKVLADLYKAGTISFEAAMSKTSKPDEIQRLIGGAPPQAQAAKTPVRAH

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=68915 CDC37_RS14580 WP_096622667.1 3612363..3613484(+) (pilT) [Calothrix sp. NIES-3974]
ATGGATTACATGATTGAAGATTTGATGGAAGATATGATTGAGCAAGGCGGCTCAGACATGCATTTATCTGCGGGTTTACC
TCCCTATTTTCGTGTCAGTGGTAAACTGACACCCCAAGATAAATATGGTGTTTTAAGCGTTGATAATTGCCAAAGATTAA
TCTTTAGTATGCTCAATAATAGCCAACGCAAAACCCTCGAACAAACTTGGGAGTTGGACTGTTCCTATGGAGTCAAAGGA
CTAGCACGTTTTCGCGTCAACGTCTATAAAGACCGGGGAGCCTATGCAGCTTGTTTACGTGCTTTAAGTTCAAAAATTCC
TAACTTTGATAAATTAGGTTTACCCGATGTCGTTCGAGAAATGTCGGAAAAACCACGGGGATTAATCTTGGTGACAGGAC
CCACTGGTTCAGGAAAAACCACCACATTAGCTGCCATGATTGACTTAATCAATCGTACCCGTGCCGAACATATTCTCACC
GTTGAAGACCCGATTGAGTTTGTCTATGAACCAATTAAAAGCCTGATTCACCAACGTCAATTAGGGGAAGATACAAAAAG
TTTTGCCAATGCTTTAAAAGCAGCTTTGCGGGAAGATCCAGATATTATTCTTGTGGGGGAAATGCGGGATTTAGAAACAA
TTTCCCTCGCAATTTCCGCCGCCGAAACTGGACACTTGGTATTTGGAACTCTCCACACTAGTTCCGCCGCACAAACCGTA
GACCGGATTATTGACGTATTTCCCACGGAAAGACAACAACAGGTACGGGTTCAGTTATCTAACTCCTTGGTAGCCGTATT
TAGTCAAACTTTAGTTCCTCGGAAAAATCCTAAACCTGGCGAATTCGGTCGGGTCATGGCTCAGGAAATTATGATTGTTA
CTCCTGCTATTTCTAACTTGATTCGGGAAGGGAAAACAGCACAAATTTACTCCGCAATTCAAACGGGAGGAAAATTAGGA
ATGCAAACCTTGGAAAAGGTTTTAGCTGATTTATATAAGGCGGGAACCATTTCCTTTGAAGCTGCTATGTCTAAAACTTC
TAAACCTGATGAAATCCAACGTCTTATCGGTGGTGCACCACCCCAGGCGCAGGCTGCTAAAACTCCGGTACGCGCCCATT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z4NKS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.624

92.761

0.507
  pilT Pseudomonas stutzeri DSM 10701

53.714

93.834

0.504
  pilT Acinetobacter baylyi ADP1

53.161

93.298

0.496
  pilT Legionella pneumophila strain Lp02

52.137

94.102

0.491
  pilT Legionella pneumophila strain ERS1305867

52.137

94.102

0.491
  pilT Acinetobacter baumannii strain A118

52.312

92.761

0.485
  pilT Acinetobacter nosocomialis M2

52.312

92.761

0.485
  pilT Acinetobacter baumannii D1279779

52.312

92.761

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.288

94.102

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.146

91.957

0.461
  pilT Vibrio cholerae strain A1552

50.146

91.957

0.461
  pilT Neisseria gonorrhoeae MS11

50.592

90.617

0.458
  pilT Neisseria meningitidis 8013

50.296

90.617

0.456
  pilU Acinetobacter baylyi ADP1

39.045

95.442

0.373
  pilU Pseudomonas stutzeri DSM 10701

38.636

94.37

0.365

Multiple sequence alignment