Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CDC37_RS14575 Genome accession   NZ_AP018254
Coordinates   3610074..3612086 (+) Length   670 a.a.
NCBI ID   WP_096622666.1    Uniprot ID   -
Organism   Calothrix sp. NIES-3974     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3605074..3617086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDC37_RS14560 cas2 3607054..3607215 (-) 162 Protein_2924 CRISPR-associated endonuclease Cas2 -
  CDC37_RS24390 - 3607654..3607809 (+) 156 WP_157780757.1 hypothetical protein -
  CDC37_RS14565 (NIES3974_29770) - 3607802..3608581 (+) 780 WP_096622664.1 hypothetical protein -
  CDC37_RS14570 (NIES3974_29780) - 3608621..3609763 (-) 1143 WP_096622665.1 quinone-dependent dihydroorotate dehydrogenase -
  CDC37_RS14575 (NIES3974_29790) pilB/pilB1 3610074..3612086 (+) 2013 WP_096622666.1 GspE/PulE family protein Machinery gene
  CDC37_RS14580 (NIES3974_29800) pilT 3612363..3613484 (+) 1122 WP_096622667.1 type IV pilus twitching motility protein PilT Machinery gene
  CDC37_RS14585 (NIES3974_29810) - 3613574..3614788 (+) 1215 WP_096622668.1 type II secretion system F family protein -
  CDC37_RS14590 (NIES3974_29820) - 3614938..3615471 (+) 534 WP_096626235.1 hypothetical protein -
  CDC37_RS14595 (NIES3974_29830) - 3615486..3616031 (-) 546 WP_096622669.1 hypothetical protein -
  CDC37_RS14600 (NIES3974_29840) hisA 3616252..3617025 (-) 774 WP_096626237.1 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74981.78 Da        Isoelectric Point: 5.1159

>NTDB_id=68914 CDC37_RS14575 WP_096622666.1 3610074..3612086(+) (pilB/pilB1) [Calothrix sp. NIES-3974]
MTYSSPQRRSVALTTRTEFSPFGNKLVQSGFVNSDQMRQALIESRKSGRPLTEVVESISGRQLSPELLRLYKKQQLFELK
ILYGVDSLDPEINTIGNSLVGQLIETLIPVDICRRHRLVPLSKNEEENPPSVLVAMVDPDNLEASDDLNRILRPQGLSLR
RMVITQEDYQQIINRYLDDLAVKQKHIEQERYTDINQDLENLGDLDLADAPEEGEVDLGSAMKGAEDAPIINLVNRILAK
ALQDKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIIAQLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSATQLGLDKLITDPETLSIVKDMVSRPFGLILVTGPTGSGKTTSLYSALAERNEPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMISSSLIGVLAQRLVRRVCPDCRIPYTPTTEELARYGLSASQTEGVIFYKANSLSNEQIQTAKNNNTLCPTCNG
VGYKGRCGVYEVMRVTEQLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVKQGQTTLEEVERVTFTDTGLEAELKAKR
KSGLTCRTCHAQLQPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=68914 CDC37_RS14575 WP_096622666.1 3610074..3612086(+) (pilB/pilB1) [Calothrix sp. NIES-3974]
ATGACTTATTCGTCCCCCCAAAGACGTAGTGTTGCTCTGACTACACGAACGGAATTTTCGCCGTTTGGTAACAAGTTAGT
TCAATCAGGGTTTGTCAACAGCGACCAAATGCGGCAAGCTTTAATCGAAAGCCGCAAATCCGGTAGACCCTTAACGGAGG
TGGTTGAATCCATCTCCGGTCGCCAATTGTCACCGGAATTATTAAGACTGTATAAAAAGCAACAATTATTTGAACTCAAA
ATCCTGTACGGAGTTGATTCCCTTGACCCAGAAATTAACACCATTGGTAATTCCTTGGTTGGTCAATTAATCGAAACTCT
GATTCCAGTGGATATTTGTCGTCGTCATCGTTTGGTTCCCCTCTCCAAAAATGAGGAGGAAAATCCGCCATCGGTGTTAG
TTGCGATGGTTGATCCAGACAACTTGGAAGCATCCGACGACTTGAATCGGATTTTGCGTCCCCAGGGTTTGAGCTTGCGA
CGCATGGTAATCACCCAGGAGGATTACCAACAAATTATTAACCGTTATTTGGATGATTTAGCGGTTAAACAGAAACATAT
TGAACAGGAACGCTATACCGACATCAACCAAGACCTGGAAAACCTTGGTGATTTGGATTTAGCGGATGCACCAGAAGAAG
GGGAAGTTGACCTTGGTTCTGCAATGAAGGGAGCAGAAGATGCACCCATCATTAACCTGGTCAACCGAATTCTGGCGAAA
GCTCTACAAGATAAGGTTTCAGACATTCATATTGAACCCCAAGAAGAAAACCTCCGCATCCGTTTTCGTAAAGACGGGGT
ATTGCGGGAAGCCTTCGACCCCCTACCGAAAAAAATTATTCCGGCTGTCACAGCCCGTTTTAAAATTATTGCCCAACTAG
ACATCGCCGAACGTCGTTTACCCCAGGATGGTCGGATTCGGCGGATGTTTGAAGGTAGAAAAGTTGACTTTCGGGTCAAT
ACCCTACCTAGTCGCTACGGTGAAAAAGTCGTATTGCGGATTTTAGATAACTCTGCCACCCAATTAGGCTTAGATAAATT
AATTACCGACCCGGAGACCCTTTCCATTGTCAAAGATATGGTATCTCGTCCCTTCGGCTTGATTTTGGTGACAGGACCTA
CGGGTTCCGGAAAAACCACCTCCTTATATTCAGCACTGGCTGAACGGAACGAACCAGGGATTAATATTAGTACGGTAGAG
GACCCGATTGAGTATAGTTTGCCAGGGATTACCCAAGTCCAGGTGATTCGGGAAAAGGGTTTAGATTTTGCCACCGCTTT
GCGAGCATTTTTGCGTCAAGACCCGGATGTCTTACTCGTTGGGGAAACACGAGACAAAGAAACAGCTAAAACCGCCATCG
AAGCCGCATTAACTGGACACTTAGTTTTGACCACCCTCCACACCAACGATGCACCGGGTGCGATCGCCCGTTTAGGGGAA
ATGGGTATCGAACCATTCATGATTTCCAGTTCTCTCATTGGTGTACTAGCACAACGTTTAGTCAGACGGGTTTGTCCCGA
TTGTCGGATTCCCTACACACCCACCACTGAAGAATTAGCCCGCTACGGTCTATCTGCTTCCCAAACCGAAGGGGTCATTT
TCTATAAAGCGAATAGTCTCAGTAATGAGCAAATTCAAACTGCGAAAAATAATAATACCCTCTGTCCTACTTGTAATGGG
GTGGGTTACAAAGGTCGATGTGGTGTGTATGAGGTGATGCGAGTTACCGAACAATTACAAACACTTATTAACGAAGAAGC
ACCCACAGAACGGATCAAAGAAGTAGCTGTAGAGGAGGGAATGAAAACCTTACTTGCCTATAGTTTAGATTTGGTCAAAC
AAGGACAAACCACCCTGGAAGAAGTTGAACGGGTAACGTTTACCGATACTGGTTTAGAAGCGGAACTCAAAGCGAAACGT
AAAAGCGGTCTAACCTGTCGAACATGTCATGCTCAACTCCAACCAGAATGGCTAGATTGTCCCTATTGTATGACCCCCAG
ATTCCAAGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.566

99.104

0.66
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.978

93.433

0.364

Multiple sequence alignment