Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5D45_RS14040 Genome accession   NZ_CP097330
Coordinates   2972163..2973206 (-) Length   347 a.a.
NCBI ID   WP_144202484.1    Uniprot ID   A0AAE9HY79
Organism   Cupriavidus campinensis strain G5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2967163..2978206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5D45_RS14015 (M5D45_14015) - 2968429..2968818 (+) 390 WP_092298902.1 CidA/LrgA family protein -
  M5D45_RS14020 (M5D45_14020) - 2968815..2969540 (+) 726 WP_144202476.1 LrgB family protein -
  M5D45_RS14025 (M5D45_14025) - 2969727..2970419 (+) 693 WP_144202478.1 helix-turn-helix transcriptional regulator -
  M5D45_RS14030 (M5D45_14030) - 2970453..2970947 (-) 495 WP_144202480.1 glutathione peroxidase -
  M5D45_RS14035 (M5D45_14035) - 2971009..2972147 (-) 1139 Protein_2759 PilT/PilU family type 4a pilus ATPase -
  M5D45_RS14040 (M5D45_14040) pilT 2972163..2973206 (-) 1044 WP_144202484.1 type IV pilus twitching motility protein PilT Machinery gene
  M5D45_RS14045 (M5D45_14045) - 2973272..2973961 (+) 690 WP_144202486.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5D45_RS14050 (M5D45_14050) proC 2974041..2974871 (+) 831 WP_144202488.1 pyrroline-5-carboxylate reductase -
  M5D45_RS14055 (M5D45_14055) ubiA 2974931..2975824 (-) 894 WP_144202490.1 4-hydroxybenzoate octaprenyltransferase -
  M5D45_RS14060 (M5D45_14060) - 2975913..2976398 (-) 486 WP_092298875.1 Dps family protein -
  M5D45_RS14065 (M5D45_14065) - 2976489..2976683 (-) 195 WP_144202492.1 hypothetical protein -
  M5D45_RS14070 (M5D45_14070) - 2976892..2977848 (-) 957 WP_092298869.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38379.15 Da        Isoelectric Point: 6.7409

>NTDB_id=688627 M5D45_RS14040 WP_144202484.1 2972163..2973206(-) (pilT) [Cupriavidus campinensis strain G5]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMGHKDVHAMVYDIMSDVQRKSYEERLEVDFSFEIAGLSR
FRVNAYTTQRGSAAVFRTIPSKVLTLDDLHAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDEDMGHILTVED
PIEFVHNSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGMGMQTLDQC
LSDLIKRGMINYNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=688627 M5D45_RS14040 WP_144202484.1 2972163..2973206(-) (pilT) [Cupriavidus campinensis strain G5]
ATGGACATCGCGCAGCTATTGGCTTTTGCGGTCAAGAACAAGGCATCCGATCTCCACCTCTCGGCAGACATGCCGCCGAT
GGTGCGTATTCACGGGGATATGCGTCGGATCAACGTGGCGGCCATGGGCCACAAGGACGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTTCAGCGCAAGAGCTACGAAGAACGCCTCGAGGTCGACTTCTCGTTCGAGATTGCCGGCCTGTCACGG
TTCCGGGTCAATGCCTACACCACGCAGCGCGGTTCTGCCGCTGTGTTCCGGACCATTCCGTCGAAAGTCCTGACGCTCGA
TGACCTCCATGCACCGGCCGTGTTCGCGGACCTCTGCATGAAGCCACGCGGCCTCGTGCTCGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCCATGGTCGATCACCGCAACGACGAGGACATGGGTCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTCCACAACTCCAAGAAAAGCCTGATCAACCAGCGCGAACTGGGCCCGCACACGCATTCGTTCGCGAA
CGCGCTGCGCTCGGCGCTGCGTGAGGATCCGGACGTGATCCTGGTGGGCGAATTGCGTGACCTGGAAACCATCCGGCTGG
CGTTGACCGCCGCCGAAACCGGCCACCTGGTCTTCGCCACGCTGCACACGAGTTCCGCCGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCCCCGAGGAAAAGGACATGGTGCGGACCATGCTGTCGGAATCGCTCGAGGCCGTGATCTCCCAGAC
GCTGCTCAAGACGCGCGACGGCAACGGCCGGACCGCGGCGCACGAGATCATGATCGCCACGCCCGCCATCCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTATTCGATGATGCAGACCTCGAGCGGCATGGGCATGCAGACGCTTGACCAGTGC
CTGAGCGATCTGATCAAACGCGGCATGATCAACTACAACGACGCGCGGGCGATTGCCAAGAATCCCGACGCCTTCATGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

70.52

99.712

0.703
  pilT Acinetobacter baumannii D1279779

70.52

99.712

0.703
  pilT Acinetobacter baumannii strain A118

70.52

99.712

0.703
  pilT Acinetobacter baylyi ADP1

68.986

99.424

0.686
  pilT Pseudomonas stutzeri DSM 10701

68.986

99.424

0.686
  pilT Pseudomonas aeruginosa PAK

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

66.57

99.135

0.66
  pilT Neisseria meningitidis 8013

66.57

99.135

0.66
  pilT Legionella pneumophila strain Lp02

65.797

99.424

0.654
  pilT Legionella pneumophila strain ERS1305867

65.797

99.424

0.654
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.579

97.118

0.637
  pilT Vibrio cholerae strain A1552

65.579

97.118

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

99.424

0.487
  pilU Acinetobacter baylyi ADP1

40.698

99.135

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389
  pilU Vibrio cholerae strain A1552

40

96.542

0.386