Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M4I31_RS03935 Genome accession   NZ_CP097324
Coordinates   802677..803657 (+) Length   326 a.a.
NCBI ID   WP_085048751.1    Uniprot ID   -
Organism   Citrobacter sp. XT1-2-2 strain XT1_2_2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 797677..808657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4I31_RS03905 (M4I31_03905) ansB 798190..799236 (+) 1047 WP_038633619.1 L-asparaginase 2 -
  M4I31_RS03910 (M4I31_03910) hemW 799353..800489 (-) 1137 WP_085048748.1 radical SAM family heme chaperone HemW -
  M4I31_RS03915 (M4I31_03915) - 800482..801075 (-) 594 WP_085048749.1 XTP/dITP diphosphatase -
  M4I31_RS03920 (M4I31_03920) yggU 801083..801373 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  M4I31_RS03925 (M4I31_03925) - 801370..801936 (-) 567 WP_038633609.1 YggT family protein -
  M4I31_RS03930 (M4I31_03930) - 801955..802659 (-) 705 WP_085048750.1 YggS family pyridoxal phosphate-dependent enzyme -
  M4I31_RS03935 (M4I31_03935) pilT 802677..803657 (+) 981 WP_085048751.1 type IV pilus twitching motility protein PilT Machinery gene
  M4I31_RS03940 (M4I31_03940) ruvX 803654..804070 (-) 417 WP_038633601.1 Holliday junction resolvase RuvX -
  M4I31_RS03945 (M4I31_03945) - 804070..804633 (-) 564 WP_032940453.1 YqgE/AlgH family protein -
  M4I31_RS03950 (M4I31_03950) gshB 804745..805692 (-) 948 WP_161668222.1 glutathione synthase -
  M4I31_RS03955 (M4I31_03955) rsmE 805705..806436 (-) 732 WP_038633593.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M4I31_RS03960 (M4I31_03960) endA 806511..807218 (-) 708 WP_085048753.1 deoxyribonuclease I -
  M4I31_RS03965 (M4I31_03965) - 807313..807810 (-) 498 WP_038633587.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35863.15 Da        Isoelectric Point: 6.8709

>NTDB_id=688407 M4I31_RS03935 WP_085048751.1 802677..803657(+) (pilT) [Citrobacter sp. XT1-2-2 strain XT1_2_2]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGNVEIAPFTTPDVETLLMCWLSEQQQVQWQKQGQLDFAITLTNSRRLRA
SAFVHQQGISLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVAYLNQHVAGHILTLEDPIE
YRHTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAPEKDPVRNQLADSLRAVLSQKLEQDKQGGRVALFELLINTPAVGNLIREGKTHQLPGVMQTGQQTGMQTFTQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=688407 M4I31_RS03935 WP_085048751.1 802677..803657(+) (pilT) [Citrobacter sp. XT1-2-2 strain XT1_2_2]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACGCGGAAATGTCGAAATCGCGCCGTTTACCACGCCTGACGTAGAGACGTTGCTGATGTGCTGGCTCAGCG
AGCAACAACAGGTACAGTGGCAGAAACAGGGGCAGCTGGATTTTGCCATTACGCTGACGAACTCACGGCGTCTGCGTGCG
AGCGCATTTGTTCATCAGCAGGGAATCTCTCTGGCGCTAAGGCTATTGCCGCTTGATTGCCCATGTTTGGATGATCTCCA
GACTCCCGCCGCCTTACCTGAGCTGCTGCACAGTGAAAATGGATTAATTCTGGTGACCGGCGCTACCGGCAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGGCATATCTTAATCAGCACGTCGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGTCACACCAGCCAGCGTTGTCTCATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTACGCGAAGATCCTGATGTCATTCTGCTGGGCGAGCTGCGCGACGTAGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACACACGCGAGGGGCGGCTCAGGCGATTGCGCGGCTGGTGGATACT
TTTCCCGCACCGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAACA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCATCAACACGCCCGCCGTCGGTAACCTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATGCAAACCGGGCAACAGACAGGCATGCAGACGTTTACGCAAAGCCTGCAGCAGCGC
CAGGCGCAGGGACGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.745

98.466

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.077

99.693

0.39
  pilU Vibrio cholerae strain A1552

40.645

95.092

0.387