Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M2M59_RS10285 Genome accession   NZ_CP097314
Coordinates   2053015..2054058 (-) Length   347 a.a.
NCBI ID   WP_249774518.1    Uniprot ID   -
Organism   Rummeliibacillus sp. G93     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2048015..2059058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2M59_RS10260 (M2M59_10260) - 2048664..2049521 (-) 858 WP_249774513.1 hypothetical protein -
  M2M59_RS10265 (M2M59_10265) - 2049518..2050105 (-) 588 WP_249774514.1 hypothetical protein -
  M2M59_RS10270 (M2M59_10270) pilM 2050102..2051082 (-) 981 WP_249774515.1 type IV pilus biogenesis protein PilM -
  M2M59_RS10275 (M2M59_10275) - 2051228..2051773 (-) 546 WP_249774516.1 type II secretion system protein -
  M2M59_RS10280 (M2M59_10280) - 2051810..2053018 (-) 1209 WP_249774517.1 type II secretion system F family protein -
  M2M59_RS10285 (M2M59_10285) pilT 2053015..2054058 (-) 1044 WP_249774518.1 type IV pilus twitching motility protein PilT Machinery gene
  M2M59_RS10290 (M2M59_10290) - 2054070..2055734 (-) 1665 WP_249774519.1 GspE/PulE family protein -
  M2M59_RS10295 (M2M59_10295) - 2055746..2057146 (-) 1401 WP_249774520.1 VanW family protein -
  M2M59_RS10300 (M2M59_10300) - 2057548..2057982 (+) 435 WP_249774521.1 hypothetical protein -
  M2M59_RS10305 (M2M59_10305) - 2057986..2058447 (+) 462 WP_249774522.1 type II secretion system protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38505.19 Da        Isoelectric Point: 6.9339

>NTDB_id=688373 M2M59_RS10285 WP_249774518.1 2053015..2054058(-) (pilT) [Rummeliibacillus sp. G93]
MTKSIVELLTYADEANASDLHATVGVPPYYRINGALQPYGEQILTPDDLNKMILEVLPPHRMEEFETKGEVDFNYSLPGR
CRFRMNAYHQRNSSTLAARKITAEIPTIDDLGMPAVLKELCQKPQGLILVTGPTGSGKSTTLAAMIDYINKHQAKHIITL
EDPIEYLHKHGQSIVNQREVGSDTRSFANGLRAALRQDPDIILVGEMRDLETISTAITAAETGHLVMATLHTSSAPTTID
RIIDVFPPHQQGQIRIQLANVLQGIISQRLFKRIDKPGRVAATEILIQTPSVANLIRNDKVHQLTNIMQTNRALGMHTID
SSIQQLLAKRLIDFETAKVYMNAGDFA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=688373 M2M59_RS10285 WP_249774518.1 2053015..2054058(-) (pilT) [Rummeliibacillus sp. G93]
ATGACAAAATCAATCGTTGAATTACTAACATATGCAGATGAAGCCAATGCATCCGACCTGCATGCCACTGTCGGCGTGCC
ACCATATTACAGAATCAATGGAGCATTGCAGCCATATGGGGAACAAATACTAACGCCGGATGATTTAAATAAGATGATTT
TAGAAGTTTTGCCACCTCATCGAATGGAAGAATTCGAGACAAAAGGTGAAGTAGACTTTAACTATTCACTTCCAGGGCGA
TGCCGTTTTCGAATGAATGCCTATCATCAGCGAAATAGCTCCACATTGGCAGCGAGGAAAATCACAGCGGAAATTCCTAC
AATCGATGATTTGGGCATGCCTGCTGTATTAAAAGAATTGTGTCAAAAACCTCAGGGACTCATTCTTGTGACAGGGCCAA
CAGGATCAGGTAAATCAACGACACTAGCAGCAATGATTGATTATATTAACAAGCATCAAGCGAAACACATCATTACATTA
GAGGACCCTATTGAATATTTGCATAAGCATGGACAATCGATTGTTAATCAGCGAGAAGTAGGATCTGATACTAGGAGCTT
TGCCAATGGACTGCGAGCAGCTCTTCGCCAAGATCCAGATATTATTCTAGTAGGCGAAATGAGAGATTTAGAGACGATTT
CAACAGCTATTACAGCTGCTGAGACAGGGCATCTCGTTATGGCGACATTGCATACAAGCAGTGCGCCGACAACAATTGAC
CGTATTATTGACGTCTTCCCGCCGCATCAACAAGGCCAAATTCGTATACAGCTTGCCAATGTGTTGCAAGGCATTATTTC
TCAACGCCTGTTTAAACGGATTGACAAACCAGGGCGCGTTGCTGCTACCGAAATCTTAATTCAAACACCTTCTGTCGCCA
ATCTAATTCGCAATGACAAAGTGCATCAGCTGACTAACATTATGCAGACAAACCGAGCGCTAGGTATGCATACAATTGAT
AGTTCGATCCAACAGTTGCTCGCAAAGCGCTTAATCGACTTCGAAACAGCGAAGGTATATATGAATGCGGGTGATTTTGC
ATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.064

94.813

0.484
  pilT Vibrio cholerae strain A1552

51.064

94.813

0.484
  pilT Pseudomonas stutzeri DSM 10701

49.401

96.254

0.476
  pilT Pseudomonas aeruginosa PAK

49.401

96.254

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.547

95.389

0.473
  pilT Acinetobacter baylyi ADP1

48.204

96.254

0.464
  pilT Legionella pneumophila strain Lp02

48.348

95.965

0.464
  pilT Legionella pneumophila strain ERS1305867

48.348

95.965

0.464
  pilT Acinetobacter baumannii D1279779

47.605

96.254

0.458
  pilT Acinetobacter baumannii strain A118

47.605

96.254

0.458
  pilT Acinetobacter nosocomialis M2

47.305

96.254

0.455
  pilT Neisseria meningitidis 8013

46.108

96.254

0.444
  pilT Neisseria gonorrhoeae MS11

45.808

96.254

0.441
  pilU Pseudomonas stutzeri DSM 10701

42.773

97.695

0.418
  pilU Acinetobacter baylyi ADP1

40.06

95.677

0.383
  pilU Vibrio cholerae strain A1552

42.308

89.914

0.38