Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M4X66_RS20835 Genome accession   NZ_CP097286
Coordinates   4728369..4729403 (-) Length   344 a.a.
NCBI ID   WP_029243107.1    Uniprot ID   A0A0P9Y0S2
Organism   Pseudomonas viridiflava strain JACO-C-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4723369..4734403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4X66_RS20805 ruvX 4723488..4723928 (+) 441 WP_004884373.1 Holliday junction resolvase RuvX -
  M4X66_RS20810 pyrR 4724026..4724538 (+) 513 WP_004884374.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  M4X66_RS20815 - 4724551..4725555 (+) 1005 WP_004884375.1 aspartate carbamoyltransferase catalytic subunit -
  M4X66_RS20820 - 4725552..4726823 (+) 1272 WP_249582928.1 dihydroorotase -
  M4X66_RS20825 - 4726974..4727378 (-) 405 WP_058430889.1 TM2 domain-containing protein -
  M4X66_RS20830 - 4727629..4728312 (+) 684 WP_249582929.1 C40 family peptidase -
  M4X66_RS20835 pilT 4728369..4729403 (-) 1035 WP_029243107.1 type IV pilus twitching motility protein PilT Machinery gene
  M4X66_RS20840 - 4729459..4730145 (+) 687 WP_249582930.1 YggS family pyridoxal phosphate-dependent enzyme -
  M4X66_RS20845 proC 4730168..4730986 (+) 819 WP_004884381.1 pyrroline-5-carboxylate reductase -
  M4X66_RS20850 - 4731013..4731603 (+) 591 WP_025995499.1 YggT family protein -
  M4X66_RS20855 - 4731833..4732972 (+) 1140 WP_004884383.1 homoserine O-acetyltransferase -
  M4X66_RS20860 metW 4732980..4733600 (+) 621 WP_004884384.1 methionine biosynthesis protein MetW -
  M4X66_RS20865 - 4733625..4734059 (+) 435 WP_122813828.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37886.75 Da        Isoelectric Point: 7.0225

>NTDB_id=688269 M4X66_RS20835 WP_029243107.1 4728369..4729403(-) (pilT) [Pseudomonas viridiflava strain JACO-C-5]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQRQVFEESLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSRILSMEDLGMGNVFRKIADVPRGLILVTGPTGSGKSTTLAAMIDYLNCNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKSMIRSMLSESLHAVVSQSLLKKVGGGRIAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGGSMGMQTLDMCL
ADLVKKGLITRESARERAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=688269 M4X66_RS20835 WP_029243107.1 4728369..4729403(-) (pilT) [Pseudomonas viridiflava strain JACO-C-5]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAACAGGGCGCGTCGGACTTGCACCTCTCTGCAGGCCTGCCGCCGAT
GATCCGTGTCGACGGCGATGTGCGGCGGATCAACCTGCCACCGCTCGATGCAAAAGAAGTCAAGGCGCTGATCTACGACA
TCATGAATGATAAGCAGCGCCAGGTCTTCGAGGAGTCTCTGGAAACCGACTTCTCGTTCGAAGTGCCGGGTGTCGCGCGG
TTCCGCGTCAACGCGTTCAACCAGAATCGTGGCGCTGGCGCCGTGTTCCGGACCATTCCTTCCCGGATCCTGAGCATGGA
AGATTTGGGCATGGGCAATGTGTTTCGCAAGATTGCAGATGTGCCTCGCGGCCTGATTCTGGTCACGGGGCCCACCGGGT
CGGGTAAATCGACCACGCTGGCGGCGATGATCGACTACCTCAACTGCAACAAGCATCACCACATCCTGACCATCGAAGAC
CCTATCGAATTCGTTCACGAATCCAAGAAGTGCCTGGTCAACCAGCGCGAAGTGCATCGCGACACGCTCGGGTTTTCAGA
GGCTTTGCGTTCGGCACTGCGTGAAGACCCGGACGTGATTCTGGTGGGCGAGCTGCGCGACCTCGAGACAATTCGTCTGG
CGCTGACGGCGGCCGAGACCGGCCATCTGGTGTTCGGCACGCTGCACACGACATCAGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTTCCGGCTCAGGAAAAATCCATGATCCGCTCGATGCTGTCCGAGTCGCTGCACGCGGTGGTGTCGCAGTC
ACTGCTCAAGAAGGTCGGCGGCGGCCGGATCGCGGCTCATGAAATCATGATGGGCACGCCCGCGATCCGTAACCTGATTC
GCGAAGACAAGGTGGCGCAGATGTATTCGGCCATTCAGACCGGAGGCTCGATGGGCATGCAGACGCTGGACATGTGCCTG
GCCGATCTGGTCAAGAAAGGCCTGATCACCCGTGAAAGCGCACGCGAGCGGGCCAAGGTGCCTGACAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P9Y0S2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baylyi ADP1

77.035

100

0.77
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

67.647

98.837

0.669
  pilT Neisseria gonorrhoeae MS11

67.353

98.837

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.965

0.654
  pilT Vibrio cholerae strain A1552

66.766

97.965

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Acinetobacter baylyi ADP1

40.751

100

0.41
  pilU Vibrio cholerae strain A1552

40.597

97.384

0.395