Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M4270_RS21030 Genome accession   NZ_CP097228
Coordinates   4358783..4359763 (+) Length   326 a.a.
NCBI ID   WP_004174422.1    Uniprot ID   -
Organism   Klebsiella pneumoniae strain 5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4353783..4364763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4270_RS21000 (M4270_20995) - 4354114..4355160 (-) 1047 WP_043906897.1 sugar ABC transporter substrate-binding protein -
  M4270_RS21005 (M4270_21000) hemW 4355453..4356592 (-) 1140 WP_004181269.1 radical SAM family heme chaperone HemW -
  M4270_RS21010 (M4270_21005) - 4356585..4357178 (-) 594 WP_002916615.1 XTP/dITP diphosphatase -
  M4270_RS21015 (M4270_21010) yggU 4357191..4357481 (-) 291 WP_002916614.1 DUF167 family protein YggU -
  M4270_RS21020 (M4270_21015) - 4357478..4358044 (-) 567 WP_002916613.1 YggT family protein -
  M4270_RS21025 (M4270_21020) - 4358065..4358766 (-) 702 WP_004144780.1 YggS family pyridoxal phosphate-dependent enzyme -
  M4270_RS21030 (M4270_21025) pilT 4358783..4359763 (+) 981 WP_004174422.1 type IV pilus twitching motility protein PilT Machinery gene
  M4270_RS21035 (M4270_21030) - 4360112..4360765 (+) 654 WP_002916607.1 LuxR family transcriptional regulator -
  M4270_RS21040 (M4270_21035) ruvX 4360818..4361234 (-) 417 WP_002916605.1 Holliday junction resolvase RuvX -
  M4270_RS21045 (M4270_21040) - 4361234..4361797 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  M4270_RS21050 (M4270_21045) gshB 4361972..4362922 (-) 951 WP_004181268.1 glutathione synthase -
  M4270_RS21055 (M4270_21050) rsmE 4362943..4363674 (-) 732 WP_004181267.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M4270_RS21060 (M4270_21055) endA 4363766..4364473 (-) 708 WP_004181265.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35313.79 Da        Isoelectric Point: 6.9959

>NTDB_id=687932 M4270_RS21030 WP_004174422.1 4358783..4359763(+) (pilT) [Klebsiella pneumoniae strain 5]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGRLQPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALTLTCGARLRA
SAFAHTRGISLVLRLLPEQCPRLDTLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGAL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=687932 M4270_RS21030 WP_004174422.1 4358783..4359763(+) (pilT) [Klebsiella pneumoniae strain 5]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCACG
CTGGCGGCGGCAGGGCAGGCTGCAGCCCGCGCCCTTTCCCGCGCCGGATATTGCGAATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGACCCTGACCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGCCTGCTTCCTGAACAGTGCCCGCGTCTGGACACGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGGGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGATAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGCGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAACGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

100

0.518
  pilT Vibrio cholerae strain A1552

51.368

100

0.518
  pilT Acinetobacter baylyi ADP1

48.936

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii strain A118

48.024

100

0.485
  pilT Acinetobacter baumannii D1279779

48.024

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

47.72

100

0.482
  pilT Legionella pneumophila strain Lp02

47.84

99.387

0.475
  pilT Legionella pneumophila strain ERS1305867

47.84

99.387

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Vibrio cholerae strain A1552

41.358

99.387

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.875

98.466

0.393