Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MX657_RS18625 Genome accession   NZ_CP097173
Coordinates   3875332..3876312 (-) Length   326 a.a.
NCBI ID   WP_218727435.1    Uniprot ID   -
Organism   Enterobacter chuandaensis strain E20191216     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3870332..3881312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MX657_RS18595 (MX657_18590) - 3871221..3871718 (+) 498 WP_346806368.1 SprT family zinc-dependent metalloprotease -
  MX657_RS18600 (MX657_18595) endA 3871813..3872520 (+) 708 WP_346806370.1 deoxyribonuclease I -
  MX657_RS18605 (MX657_18600) rsmE 3872572..3873303 (+) 732 WP_346806372.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MX657_RS18610 (MX657_18605) gshB 3873323..3874270 (+) 948 WP_033146605.1 glutathione synthase -
  MX657_RS18615 (MX657_18610) - 3874345..3874905 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  MX657_RS18620 (MX657_18615) ruvX 3874905..3875321 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  MX657_RS18625 (MX657_18620) pilT 3875332..3876312 (-) 981 WP_218727435.1 type IV pilus twitching motility protein PilT Machinery gene
  MX657_RS18630 (MX657_18625) - 3876330..3877031 (+) 702 WP_080330043.1 YggS family pyridoxal phosphate-dependent enzyme -
  MX657_RS18635 (MX657_18630) - 3877053..3877619 (+) 567 WP_080330042.1 YggT family protein -
  MX657_RS18640 (MX657_18635) yggU 3877616..3877912 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  MX657_RS18645 (MX657_18640) - 3877916..3878509 (+) 594 WP_048979374.1 XTP/dITP diphosphatase -
  MX657_RS18650 (MX657_18645) hemW 3878502..3879644 (+) 1143 WP_346806377.1 radical SAM family heme chaperone HemW -
  MX657_RS18655 (MX657_18650) - 3879669..3879788 (+) 120 Protein_3644 DUF559 domain-containing protein -
  MX657_RS18660 (MX657_18655) - 3879884..3880600 (-) 717 WP_346806379.1 DUF2884 domain-containing protein -
  MX657_RS18665 (MX657_18660) - 3880658..3880984 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35503.57 Da        Isoelectric Point: 6.4994

>NTDB_id=687618 MX657_RS18625 WP_218727435.1 3875332..3876312(-) (pilT) [Enterobacter chuandaensis strain E20191216]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGNQRLRG
SAFKQMKGVSVTLRLLPRSCPQLASLGVPRALPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNRQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRGALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=687618 MX657_RS18625 WP_218727435.1 3875332..3876312(-) (pilT) [Enterobacter chuandaensis strain E20191216]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGACTAGGCCGCCTTGAACCGGCCCCGTTTCCGCCGCCTGACGTCGGGGCGTTATTAAAGGCGTGGCTGAACG
ACGAGCAGCAGGGAGCCTGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAAACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCGTCTCCGTTACCCTGCGCCTGTTGCCGCGTTCCTGTCCGCAGCTCGCTTCGCTGGG
TGTGCCGCGGGCGCTTCCCGAACTGTTATCCAGTGACAACGGGTTGATTCTGGTGACGGGGGCCACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCGATGGTCGATTTTCTCAACCGCCAGACTGACGGCCATATCCTCACGCTCGAAGATCCGGTGGAG
TTTATGTATCAGAGTGAACGTTGCCTGATTCAGCAGCGCGAGATTGGGCAACACAGCCCGTCCTTTGCCGAGGCGCTGCG
CGGCGCATTACGCGAGGATCCGGACGTTATTTTGCTGGGAGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGTGGGGCAGCGCAGGCAATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGAGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTCCAGGGCGGGCGCGTCGCGCTATATGAACTGTTGGTGAACACCGCTGCTGCGGCGAATCTGATTCGTGAAGGGA
AGACGTGGCAACTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGCATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362