Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M3M55_RS12550 Genome accession   NZ_CP097128
Coordinates   2638100..2639197 (-) Length   365 a.a.
NCBI ID   WP_249009845.1    Uniprot ID   -
Organism   Conexibacter sp. DBS9H8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2633100..2644197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3M55_RS12530 - 2634097..2635299 (-) 1203 WP_249009841.1 glycosyltransferase -
  M3M55_RS12535 - 2635316..2636521 (-) 1206 WP_249009842.1 glycosyltransferase family 2 protein -
  M3M55_RS12540 - 2636611..2636796 (-) 186 WP_249009843.1 hypothetical protein -
  M3M55_RS12545 - 2636862..2638070 (-) 1209 WP_249009844.1 type II secretion system F family protein -
  M3M55_RS12550 pilT 2638100..2639197 (-) 1098 WP_249009845.1 type IV pilus twitching motility protein PilT Machinery gene
  M3M55_RS12555 - 2639271..2641094 (-) 1824 WP_249009846.1 GspE/PulE family protein -
  M3M55_RS12560 - 2641091..2641915 (-) 825 WP_249009847.1 shikimate dehydrogenase -
  M3M55_RS12565 mltG 2641908..2643182 (-) 1275 WP_249009848.1 endolytic transglycosylase MltG -
  M3M55_RS12570 - 2643267..2643749 (-) 483 WP_249009849.1 CHRD domain-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 39539.42 Da        Isoelectric Point: 6.7761

>NTDB_id=687387 M3M55_RS12550 WP_249009845.1 2638100..2639197(-) (pilT) [Conexibacter sp. DBS9H8]
MSIDFADVLLQVVDRRASDLHLTAGAPPMLRMRGRLTPLEGYPILAPSDTREIIYSILTSAQRQRLETNWQLDFAYAVPG
SARFRVNAYFQRGALGAAFRLIPFEIAPLDSLGLPPVLREFANKPRGLVLVTGPTGSGKSTTLASIIDVINQTREEHIMT
IEDPIEFLHQHKRCIVNQRELGADAATFALALKAALRQDPDVILVGEMRDVETIGTAITAAETGHLVFATLHTQDAPQTI
DRIVDSFPAEQQGQIRAQLSIALQGIVTQTLVPTADGSGRAVAAEVMVPTPAVRNLVREAKTHQLYSVIQTGSQHGMQTM
DASLAGLVRTGKITKAVAEQRAHSVEELNRLLNAPSLSVAAPVAM

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=687387 M3M55_RS12550 WP_249009845.1 2638100..2639197(-) (pilT) [Conexibacter sp. DBS9H8]
ATGTCGATCGACTTCGCCGATGTCCTCCTCCAGGTGGTCGACCGGCGGGCGTCAGACCTTCACCTCACTGCCGGCGCCCC
GCCGATGCTGCGCATGCGCGGACGGCTCACCCCGCTTGAGGGGTATCCGATCCTCGCGCCGTCCGACACACGGGAGATCA
TCTACTCGATCCTCACGAGTGCCCAGCGCCAGCGGCTGGAGACCAACTGGCAGCTCGACTTCGCCTACGCGGTGCCCGGC
AGCGCCCGCTTCCGGGTCAACGCCTACTTCCAGCGCGGCGCCCTGGGTGCCGCGTTCCGCCTGATCCCGTTTGAGATCGC
CCCGCTCGACAGCCTCGGCCTGCCGCCGGTGCTGCGCGAGTTCGCCAACAAGCCGCGCGGGCTTGTGCTCGTCACCGGTC
CGACCGGGTCGGGGAAGTCCACGACGCTCGCGTCGATCATTGACGTGATCAACCAGACGCGGGAAGAGCACATCATGACG
ATCGAGGACCCGATCGAGTTCCTCCACCAGCACAAGCGCTGCATCGTCAACCAGCGCGAGCTCGGGGCTGACGCGGCGAC
CTTCGCCCTCGCGCTGAAGGCGGCATTGCGCCAGGACCCGGACGTGATCCTCGTCGGGGAGATGCGCGACGTCGAGACGA
TCGGAACGGCGATCACCGCGGCGGAGACCGGCCACCTCGTCTTCGCCACCCTCCACACCCAGGACGCCCCCCAGACGATC
GACCGGATCGTCGACTCCTTTCCGGCCGAGCAGCAGGGCCAGATCCGCGCGCAGCTGTCGATCGCCCTTCAGGGGATCGT
CACCCAGACCCTCGTCCCGACCGCGGACGGCTCCGGCCGGGCGGTCGCCGCAGAGGTGATGGTCCCGACTCCGGCGGTGC
GCAACCTCGTGCGGGAGGCCAAGACCCACCAGCTCTACTCGGTGATCCAGACCGGCTCCCAGCACGGGATGCAGACGATG
GACGCCTCCCTCGCCGGCCTCGTACGCACCGGAAAGATCACCAAGGCGGTCGCCGAGCAGCGCGCCCACTCGGTTGAGGA
GCTCAACCGGCTGCTCAACGCGCCGTCCCTGTCGGTTGCCGCCCCCGTGGCGATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.802

96.986

0.532
  pilT Legionella pneumophila strain Lp02

52.647

93.151

0.49
  pilT Legionella pneumophila strain ERS1305867

52.647

93.151

0.49
  pilT Acinetobacter baylyi ADP1

51.156

94.795

0.485
  pilT Pseudomonas aeruginosa PAK

51.765

93.151

0.482
  pilT Acinetobacter baumannii D1279779

50.867

94.795

0.482
  pilT Acinetobacter baumannii strain A118

50.867

94.795

0.482
  pilT Acinetobacter nosocomialis M2

50.867

94.795

0.482
  pilT Pseudomonas stutzeri DSM 10701

50.578

94.795

0.479
  pilT Neisseria meningitidis 8013

49.28

95.068

0.468
  pilT Neisseria gonorrhoeae MS11

48.991

95.068

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.848

90.137

0.449
  pilT Vibrio cholerae strain A1552

49.848

90.137

0.449
  pilU Pseudomonas stutzeri DSM 10701

42.938

96.986

0.416
  pilU Acinetobacter baylyi ADP1

42.075

95.068

0.4
  pilU Vibrio cholerae strain A1552

42.604

92.603

0.395