Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M3M50_RS28725 Genome accession   NZ_CP097108
Coordinates   6472313..6473347 (-) Length   344 a.a.
NCBI ID   WP_109752393.1    Uniprot ID   -
Organism   Pseudomonas bijieensis strain SP1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6467313..6478347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3M50_RS28695 (M3M50_28695) ruvX 6467408..6467845 (+) 438 WP_109752386.1 Holliday junction resolvase RuvX -
  M3M50_RS28700 (M3M50_28700) pyrR 6467931..6468437 (+) 507 WP_109752388.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  M3M50_RS28705 (M3M50_28705) - 6468462..6469466 (+) 1005 WP_003177495.1 aspartate carbamoyltransferase catalytic subunit -
  M3M50_RS28710 (M3M50_28710) - 6469463..6470734 (+) 1272 WP_109752389.1 dihydroorotase -
  M3M50_RS28715 (M3M50_28715) - 6470858..6471286 (-) 429 WP_053188929.1 TM2 domain-containing protein -
  M3M50_RS28720 (M3M50_28720) - 6471537..6472148 (+) 612 WP_109752391.1 C40 family peptidase -
  M3M50_RS28725 (M3M50_28725) pilT 6472313..6473347 (-) 1035 WP_109752393.1 type IV pilus twitching motility protein PilT Machinery gene
  M3M50_RS28730 (M3M50_28730) - 6473404..6474090 (+) 687 WP_109752395.1 YggS family pyridoxal phosphate-dependent enzyme -
  M3M50_RS28735 (M3M50_28735) proC 6474126..6474944 (+) 819 WP_109752397.1 pyrroline-5-carboxylate reductase -
  M3M50_RS28740 (M3M50_28740) - 6474955..6475545 (+) 591 WP_109752399.1 YggT family protein -
  M3M50_RS28745 (M3M50_28745) - 6475550..6475840 (+) 291 WP_116833661.1 DUF167 domain-containing protein -
  M3M50_RS28750 (M3M50_28750) - 6476058..6477197 (+) 1140 WP_109752403.1 homoserine O-acetyltransferase -
  M3M50_RS28755 (M3M50_28755) metW 6477205..6477825 (+) 621 WP_109752405.1 methionine biosynthesis protein MetW -
  M3M50_RS28760 (M3M50_28760) - 6477851..6478285 (+) 435 WP_109752407.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38108.72 Da        Isoelectric Point: 7.0158

>NTDB_id=687120 M3M50_RS28725 WP_109752393.1 6472313..6473347(-) (pilT) [Pseudomonas bijieensis strain SP1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKVTEAPRGLVLVTGPTGSGKSTTLAAMIDHLNSHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVNKGLISREHARERARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=687120 M3M50_RS28725 WP_109752393.1 6472313..6473347(-) (pilT) [Pseudomonas bijieensis strain SP1]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGGGCGTCGGACCTGCACCTGTCCGCCGGGTTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGGCGCATCAACCTGCCGGCGCTGGACCACAAGCAGGTCCATGAACTGATCTACGACA
TCATGAACGACCGGCAACGAGTAGATTACGAAAAGTTTCTGGAGACCGACTTTTCCTTCGACGTGCCGGGTGTGGCGCGG
TTCCGGGTCAACGCCTTCAACCAGAATCGCGGCGCGGGGGCGGTATTCCGGACCATTCCGTCGAAAGTCCTGACCATGGA
AGACCTGGGGATGGGCGAGGTGTTTCGCAAAGTGACCGAGGCGCCGCGCGGGCTGGTGTTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCCATGATCGACCATTTGAACAGCCACAAGCACCATCACATCCTCACCATTGAAGAC
CCTATCGAGTTCGTTCACGAACCGCGCAAATGCCTGATCAACCAGCGCGAGGTGCACCGCGACACTCAGGGGTTCTCCAC
GGCCCTGCGATCGGCCCTGCGGGAGGATCCGGACGTGATCCTGGTAGGGGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGGCATCTGGTGTTCGGCACACTGCACACCACATCGGCGGCCAAGACCATTGACCGAGTG
GTGGACGTGTTCCCCGGTGACGAGAAGTCCATGGTTCGCTCGATGTTGTCGGAATCGTTGCAGGCGGTGATTTCCCAGAC
GCTGGTCAAGAAGATCGGCGGCGGCCGGATCGCGGCGCATGAAATCATGCTGGGCACCTCGGCCATCCGGAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGTGGTTCACTGGGGATGCAGACGCTGGATATGTGTTTG
AAGGATTTGGTGAACAAAGGCTTGATCAGCCGCGAACATGCGCGGGAACGGGCGCGTTCGCCGGATAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.372

100

0.884
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

71.221

100

0.712
  pilT Legionella pneumophila strain ERS1305867

71.221

100

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Neisseria meningitidis 8013

65.217

100

0.654
  pilT Neisseria gonorrhoeae MS11

64.928

100

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.401
  pilU Vibrio cholerae strain A1552

39.71

100

0.398