Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M3L74_RS03350 Genome accession   NZ_CP097107
Coordinates   588730..589710 (+) Length   326 a.a.
NCBI ID   WP_088903072.1    Uniprot ID   -
Organism   Citrobacter freundii strain GMU8049     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 583730..594710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3L74_RS03320 (M3L74_03320) - 584336..585343 (+) 1008 WP_249018362.1 DUF1202 family protein -
  M3L74_RS03325 (M3L74_03325) hemW 585406..586542 (-) 1137 WP_088903071.1 radical SAM family heme chaperone HemW -
  M3L74_RS03330 (M3L74_03330) - 586535..587128 (-) 594 WP_003027104.1 XTP/dITP diphosphatase -
  M3L74_RS03335 (M3L74_03335) yggU 587136..587426 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  M3L74_RS03340 (M3L74_03340) - 587423..587989 (-) 567 WP_003825417.1 YggT family protein -
  M3L74_RS03345 (M3L74_03345) - 588008..588712 (-) 705 WP_003838215.1 YggS family pyridoxal phosphate-dependent enzyme -
  M3L74_RS03350 (M3L74_03350) pilT 588730..589710 (+) 981 WP_088903072.1 type IV pilus twitching motility protein PilT Machinery gene
  M3L74_RS03355 (M3L74_03355) ruvX 589707..590123 (-) 417 WP_006686843.1 Holliday junction resolvase RuvX -
  M3L74_RS03360 (M3L74_03360) - 590123..590686 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  M3L74_RS03365 (M3L74_03365) gshB 590862..591809 (-) 948 WP_003027083.1 glutathione synthase -
  M3L74_RS03370 (M3L74_03370) rsmE 591829..592560 (-) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M3L74_RS03375 (M3L74_03375) endA 592635..593342 (-) 708 WP_016150969.1 deoxyribonuclease I -
  M3L74_RS03380 (M3L74_03380) - 593437..593934 (-) 498 WP_003838223.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35906.99 Da        Isoelectric Point: 6.3638

>NTDB_id=687072 M3L74_RS03350 WP_088903072.1 588730..589710(+) (pilT) [Citrobacter freundii strain GMU8049]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRHGKVESAPFITPDVENLLMCWLSEQQQVQLQEQGQVDFAVTLTDSRRLRA
SAFVHLQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGAHCASFSAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=687072 M3L74_RS03350 WP_088903072.1 588730..589710(+) (pilT) [Citrobacter freundii strain GMU8049]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCCGCGCG
TTGGCGCAGACATGGAAAAGTCGAAAGCGCACCGTTTATCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGGTTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCTGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAATGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGCACATTGCGCCTCTTTCTCCGCCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTTGGCGAGTTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTTGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTACTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGTTTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(35-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362