Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M1L61_RS02580 Genome accession   NZ_CP096975
Coordinates   564893..566020 (-) Length   375 a.a.
NCBI ID   WP_043398800.1    Uniprot ID   A0AA42X8C5
Organism   Stenotrophomonas sp. NY11291     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 559893..571020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1L61_RS02560 (M1L61_02560) - 560374..561363 (+) 990 WP_043398812.1 fumarylacetoacetate hydrolase family protein -
  M1L61_RS02565 (M1L61_02565) maiA 561378..562061 (+) 684 WP_248854270.1 maleylacetoacetate isomerase -
  M1L61_RS02570 (M1L61_02570) - 562110..563570 (-) 1461 WP_248854271.1 UdgX family uracil-DNA binding protein -
  M1L61_RS02575 (M1L61_02575) - 563551..564834 (-) 1284 WP_049480776.1 putative DNA modification/repair radical SAM protein -
  M1L61_RS02580 (M1L61_02580) pilU 564893..566020 (-) 1128 WP_043398800.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M1L61_RS02585 (M1L61_02585) - 566142..566507 (+) 366 WP_010480631.1 DUF4398 domain-containing protein -
  M1L61_RS02590 (M1L61_02590) - 566504..567388 (+) 885 WP_010480632.1 hypothetical protein -
  M1L61_RS02595 (M1L61_02595) - 567713..567949 (+) 237 WP_005407964.1 YdcH family protein -
  M1L61_RS02600 (M1L61_02600) - 568250..569620 (+) 1371 WP_010480640.1 pyridoxal-phosphate dependent enzyme -
  M1L61_RS02605 (M1L61_02605) - 569744..570919 (+) 1176 WP_248854272.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41384.67 Da        Isoelectric Point: 6.3741

>NTDB_id=685825 M1L61_RS02580 WP_043398800.1 564893..566020(-) (pilU) [Stenotrophomonas sp. NY11291]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGTIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=685825 M1L61_RS02580 WP_043398800.1 564893..566020(-) (pilU) [Stenotrophomonas sp. NY11291]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCCTGACCACCGGAGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTTTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGGAAACGATGGAGGCCG
CCATCGCTTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATTGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCTGCGATGGAAGGCTCGCTGGAGGAAGGCATGGAGAGTTTCGACCAGTGCCTG
TTCCGGTTGGCCAAGGCTGGCACCATCGAGCAGGAGGAGGCCCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAACACGACCCGTACGCGGATTTCTCGGCCGGCGCGCCGAGCATCACCCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.471

90.667

0.376
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363