Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M0220_RS06015 Genome accession   NZ_CP096973
Coordinates   1289822..1290865 (-) Length   347 a.a.
NCBI ID   WP_264018944.1    Uniprot ID   A0AA46YPJ8
Organism   Halomonas qinghailakensis strain ZZQ-149     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1284822..1295865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0220_RS06005 (M0220_05985) tyrS 1287328..1288527 (-) 1200 WP_030074089.1 tyrosine--tRNA ligase -
  M0220_RS06010 (M0220_05990) - 1288644..1289786 (+) 1143 WP_030074091.1 anhydro-N-acetylmuramic acid kinase -
  M0220_RS06015 (M0220_05995) pilT 1289822..1290865 (-) 1044 WP_264018944.1 type IV pilus twitching motility protein PilT Machinery gene
  M0220_RS06020 (M0220_06000) - 1290900..1291616 (+) 717 WP_264018945.1 YggS family pyridoxal phosphate-dependent enzyme -
  M0220_RS06025 (M0220_06005) proC 1291694..1292518 (+) 825 WP_030074094.1 pyrroline-5-carboxylate reductase -
  M0220_RS06030 (M0220_06010) - 1292548..1293141 (+) 594 WP_009723714.1 YggT family protein -
  M0220_RS06035 (M0220_06015) - 1293206..1294402 (+) 1197 WP_030074095.1 homoserine O-acetyltransferase -
  M0220_RS06040 (M0220_06020) metW 1294402..1294995 (+) 594 WP_030074096.1 methionine biosynthesis protein MetW -
  M0220_RS06045 (M0220_06025) - 1295044..1295505 (+) 462 WP_030074097.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38087.54 Da        Isoelectric Point: 6.0049

>NTDB_id=685813 M0220_RS06015 WP_264018944.1 1289822..1290865(-) (pilT) [Halomonas qinghailakensis strain ZZQ-149]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAMDNSDVCRLIFDIMNEQQRRDYEEHLEADFSFEVSGVAR
FRVNAFHQARGAGAVFRTIPNDVLSMRNLGMGDVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRFEHILTIED
PVEFVHTSKRCLINQREVHRDTRSFTAALRGALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGEEKSMVRSMLSESLQAVISQTLLKRQGGGRIAAHEILIATAAVRNLIREDKVAQIYSAIQTGGNLGMQTLNASL
AKLVKEGVVSMEDAQAQAKGTLALSDD

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=685813 M0220_RS06015 WP_264018944.1 1289822..1290865(-) (pilT) [Halomonas qinghailakensis strain ZZQ-149]
ATGGATATTACCGAACTGCTAGCATTCTCGGCAAAGCAGAATGCATCTGACCTGCACCTTTCTGCCGGTCTTCCTCCTAT
GATACGTGTTGATGGCGATATTCGTCGGCTTAATGTACCCGCGATGGACAATAGTGACGTTTGCCGCCTGATTTTTGACA
TTATGAATGAGCAGCAGCGTCGAGACTATGAAGAGCATTTAGAAGCCGATTTTTCATTTGAAGTATCCGGGGTTGCTCGG
TTCCGTGTTAATGCATTTCATCAAGCTAGGGGGGCTGGCGCGGTGTTTCGTACTATTCCTAATGACGTGCTTTCAATGCG
CAATTTGGGAATGGGAGACGTGTTCGAGCGACTGGCCATGCTGCCAAGGGGGTTAGTGTTGGTGACTGGGCCGACGGGCT
CAGGCAAAAGCACCACACTGGCGGCCATGATTGATTATATTAACGACCACCGGTTTGAGCATATTTTAACCATCGAAGAC
CCTGTCGAGTTTGTGCATACCAGCAAGCGCTGTTTAATCAATCAGCGTGAAGTGCATCGGGATACGCGCAGCTTCACTGC
TGCATTGCGCGGTGCGTTGCGTGAAGATCCGGATGTTATTTTGGTTGGCGAACTAAGGGATCTAGAAACTATTCGTCTGG
CGCTGACGGCTGCTGAAACCGGCCACTTGGTGTTTGGTACGCTGCATACAACATCTGCGGCGAAAACGATTGACCGCATT
GTCGATGTGTTTCCCGGCGAAGAGAAATCGATGGTGCGCTCAATGTTGTCTGAGTCGCTGCAGGCGGTAATCTCCCAGAC
ATTATTAAAACGCCAGGGTGGCGGGCGTATCGCTGCGCATGAAATATTAATTGCCACTGCTGCAGTACGAAACTTAATCC
GTGAAGATAAAGTGGCTCAAATTTACTCTGCTATTCAGACCGGAGGCAATCTTGGCATGCAAACGTTAAATGCATCGCTT
GCGAAGCTTGTTAAAGAGGGTGTGGTGAGTATGGAAGATGCGCAAGCGCAGGCCAAAGGCACACTCGCGTTAAGCGATGA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.991

97.695

0.752
  pilT Pseudomonas aeruginosa PAK

76.696

97.695

0.749
  pilT Acinetobacter nosocomialis M2

74.926

97.695

0.732
  pilT Acinetobacter baumannii D1279779

74.926

97.695

0.732
  pilT Acinetobacter baumannii strain A118

74.926

97.695

0.732
  pilT Acinetobacter baylyi ADP1

73.746

97.695

0.72
  pilT Legionella pneumophila strain Lp02

69.231

97.406

0.674
  pilT Legionella pneumophila strain ERS1305867

69.231

97.406

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.118

0.674
  pilT Vibrio cholerae strain A1552

69.436

97.118

0.674
  pilT Neisseria meningitidis 8013

65.029

99.712

0.648
  pilT Neisseria gonorrhoeae MS11

64.74

99.712

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.754

95.965

0.516
  pilU Pseudomonas stutzeri DSM 10701

41.593

97.695

0.406
  pilU Acinetobacter baylyi ADP1

40.588

97.983

0.398
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392