Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M2J86_RS18900 Genome accession   NZ_CP096921
Coordinates   3900314..3901294 (+) Length   326 a.a.
NCBI ID   WP_172750735.1    Uniprot ID   -
Organism   Citrobacter freundii strain NY2385     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3895314..3906294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J86_RS18870 (M2J86_18765) ansB 3895846..3896892 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  M2J86_RS18875 (M2J86_18770) hemW 3896990..3898126 (-) 1137 WP_172750738.1 radical SAM family heme chaperone HemW -
  M2J86_RS18880 (M2J86_18775) - 3898119..3898712 (-) 594 WP_172750737.1 XTP/dITP diphosphatase -
  M2J86_RS18885 (M2J86_18780) yggU 3898720..3899010 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  M2J86_RS18890 (M2J86_18785) - 3899007..3899573 (-) 567 WP_003027097.1 YggT family protein -
  M2J86_RS18895 (M2J86_18790) - 3899592..3900296 (-) 705 WP_181624047.1 YggS family pyridoxal phosphate-dependent enzyme -
  M2J86_RS18900 (M2J86_18795) pilT 3900314..3901294 (+) 981 WP_172750735.1 type IV pilus twitching motility protein PilT Machinery gene
  M2J86_RS18905 (M2J86_18800) ruvX 3901291..3901707 (-) 417 WP_003027087.1 Holliday junction resolvase RuvX -
  M2J86_RS18910 (M2J86_18805) - 3901707..3902270 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  M2J86_RS18915 (M2J86_18810) gshB 3902382..3903329 (-) 948 WP_003027083.1 glutathione synthase -
  M2J86_RS18920 (M2J86_18815) rsmE 3903349..3904080 (-) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M2J86_RS18925 (M2J86_18820) endA 3904155..3904862 (-) 708 WP_016150969.1 deoxyribonuclease I -
  M2J86_RS18930 (M2J86_18825) - 3904957..3905454 (-) 498 WP_003846365.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35878.94 Da        Isoelectric Point: 6.3638

>NTDB_id=685202 M2J86_RS18900 WP_172750735.1 3900314..3901294(+) (pilT) [Citrobacter freundii strain NY2385]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRHGKVESAPFTAPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRA
SAFVHLQGTSLALRLLPLDCPHLDDLQPPAVIPELLHSENGVILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGAHCASFATGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=685202 M2J86_RS18900 WP_172750735.1 3900314..3901294(+) (pilT) [Citrobacter freundii strain NY2385]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCAGACATGGAAAAGTCGAAAGCGCACCGTTTACCGCGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCTGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCCCATTTAGACGATCTTCA
GCCTCCCGCGGTCATACCTGAACTGCTTCACAGTGAAAACGGGGTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTAATTCAACAGCGGGAGGTGGGCGCACACTGCGCCTCTTTCGCCACCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGAGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGATAGCCTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTTTACAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.342

98.773

0.448
  pilT Legionella pneumophila strain ERS1305867

45.342

98.773

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365