Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M2R49_RS04185 Genome accession   NZ_CP096905
Coordinates   847217..848197 (+) Length   326 a.a.
NCBI ID   WP_054176416.1    Uniprot ID   -
Organism   Citrobacter amalonaticus strain RSHH22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 842217..853197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2R49_RS04155 (M2R49_04150) - 842854..843861 (+) 1008 WP_054176415.1 DUF1202 family protein -
  M2R49_RS04160 (M2R49_04155) hemW 843893..845029 (-) 1137 WP_042998064.1 radical SAM family heme chaperone HemW -
  M2R49_RS04165 (M2R49_04160) - 845022..845615 (-) 594 WP_044263976.1 XTP/dITP diphosphatase -
  M2R49_RS04170 (M2R49_04165) yggU 845623..845913 (-) 291 WP_044255664.1 DUF167 family protein YggU -
  M2R49_RS04175 (M2R49_04170) - 845910..846476 (-) 567 WP_042998061.1 YggT family protein -
  M2R49_RS04180 (M2R49_04175) - 846495..847199 (-) 705 WP_044263978.1 YggS family pyridoxal phosphate-dependent enzyme -
  M2R49_RS04185 (M2R49_04180) pilT 847217..848197 (+) 981 WP_054176416.1 type IV pilus twitching motility protein PilT Machinery gene
  M2R49_RS04190 (M2R49_04185) ruvX 848211..848627 (-) 417 WP_042998058.1 Holliday junction resolvase RuvX -
  M2R49_RS04195 (M2R49_04190) - 848627..849190 (-) 564 WP_042998057.1 YqgE/AlgH family protein -
  M2R49_RS04200 (M2R49_04195) gshB 849365..850312 (-) 948 WP_054176417.1 glutathione synthase -
  M2R49_RS04205 (M2R49_04200) rsmE 850325..851056 (-) 732 WP_042998055.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M2R49_RS04210 (M2R49_04205) endA 851137..851844 (-) 708 WP_054176418.1 deoxyribonuclease I -
  M2R49_RS04215 (M2R49_04210) - 851936..852436 (-) 501 WP_042998053.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35892.04 Da        Isoelectric Point: 7.0211

>NTDB_id=685004 M2R49_RS04185 WP_054176416.1 847217..848197(+) (pilT) [Citrobacter amalonaticus strain RSHH22]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRMERAPFTATDITGLLADWLDEGQQAALRQNGQLDFAVSLADNQRLRA
SAFHQRQGTSLALRLLPTQCPTLETLGTPSVLPELLRSENGLILVTGATGSGKSTTLAAMVTWLNQHIDGHILTLEDPIE
YIYTSQRCLIQQREVGLHCASFAIGLRAALREDPDVILLGELRDSETIRLALTAAETGHMVLATLHTRGAAQAIERLIDS
FPAQEKDPVRSQLAGSLRAVLSQKLEPDKQEGRVALYELLINTPAAGNLIREGKSHQLPHVIQTGQQMGMMTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=685004 M2R49_RS04185 WP_054176416.1 847217..848197(+) (pilT) [Citrobacter amalonaticus strain RSHH22]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCGCG
CTGGCGAAAACGGGGACGCATGGAGCGTGCACCCTTTACCGCAACCGATATTACCGGACTGCTGGCGGACTGGCTGGATG
AGGGGCAACAGGCGGCGTTACGACAAAATGGGCAACTGGATTTCGCGGTTTCGCTCGCGGATAACCAGCGCCTGCGGGCC
AGCGCCTTTCACCAGCGGCAGGGGACGTCGCTGGCGCTACGGCTGCTGCCGACGCAGTGCCCGACGCTGGAAACGCTGGG
GACGCCTTCTGTGCTGCCCGAACTGCTCCGCAGCGAGAATGGTTTGATTCTGGTGACAGGGGCGACAGGCAGCGGAAAAT
CAACTACCTTGGCGGCGATGGTCACGTGGCTGAATCAGCATATCGATGGACATATTCTGACGCTGGAAGATCCCATCGAG
TATATCTACACCAGCCAGCGCTGCCTGATTCAGCAGCGTGAAGTAGGACTGCACTGCGCCTCATTTGCCATCGGATTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGAGAGCTACGCGACAGCGAGACCATTCGTCTGGCGCTAACGG
CGGCGGAAACGGGGCATATGGTGCTGGCGACGCTGCATACGCGCGGTGCCGCGCAGGCGATCGAGCGACTGATTGATTCT
TTTCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCAGGCAGTCTGCGCGCCGTACTGTCGCAGAAACTGGAGCC
CGACAAGCAGGAGGGGCGCGTTGCGCTGTATGAACTGCTGATCAACACGCCGGCAGCGGGGAATTTGATTCGGGAAGGGA
AGTCCCATCAGTTACCGCATGTCATTCAGACCGGGCAGCAGATGGGGATGATGACCTTTGCCCAGAGCCTGCAACAGCGA
CAGGCACAAGGACGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.038

96.933

0.417
  pilU Vibrio cholerae strain A1552

42.581

95.092

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Acinetobacter baylyi ADP1

39.385

99.693

0.393