Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M1J35_RS18340 Genome accession   NZ_CP096886
Coordinates   3573354..3574397 (-) Length   347 a.a.
NCBI ID   WP_254653796.1    Uniprot ID   -
Organism   Rossellomorea sp. KS-H15a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3568354..3579397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1J35_RS18310 (M1J35_18310) - 3568424..3569209 (-) 786 WP_254651884.1 hypothetical protein -
  M1J35_RS18315 (M1J35_18315) - 3569206..3569823 (-) 618 WP_254651885.1 hypothetical protein -
  M1J35_RS18320 (M1J35_18320) pilM 3569823..3570785 (-) 963 WP_254651886.1 type IV pilus biogenesis protein PilM -
  M1J35_RS18325 (M1J35_18325) - 3570801..3571556 (-) 756 WP_254651887.1 A24 family peptidase -
  M1J35_RS18330 (M1J35_18330) - 3571573..3572031 (-) 459 WP_254651888.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  M1J35_RS18335 (M1J35_18335) - 3572142..3573353 (-) 1212 WP_254651889.1 type II secretion system F family protein -
  M1J35_RS18340 (M1J35_18340) pilT 3573354..3574397 (-) 1044 WP_254653796.1 type IV pilus twitching motility protein PilT Machinery gene
  M1J35_RS18345 (M1J35_18345) - 3574407..3576068 (-) 1662 WP_254651890.1 GspE/PulE family protein -
  M1J35_RS18350 (M1J35_18350) - 3576090..3577475 (-) 1386 WP_254651891.1 G5 domain-containing protein -
  M1J35_RS18355 (M1J35_18355) - 3577503..3578237 (-) 735 WP_254651892.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38523.56 Da        Isoelectric Point: 7.6696

>NTDB_id=684832 M1J35_RS18340 WP_254653796.1 3573354..3574397(-) (pilT) [Rossellomorea sp. KS-H15a]
MKEKVDYLLKSAFELGASDIHLTVGSPPIFRIHGDLKRYGKENLLPDHTELMAKAIISETMYPAFEEVGELDFSYGIPGV
SRFRVNAFKQRSCVSLAIRVIPTSIPTMEDLFLPHALKAIAEKPQGLFLVTGPTGSGKSTTLASIVDYMNRKMRKHIITL
EDPIEYLHKHGASIIDQREVGFDTRSFSKGLRSALRQDPDIILVGEMRDLETIHTAITAAETGHLVLATLHTSSAPATIE
RIVDVFPPEQQSQIRVQLASVLVGILSQRLFATVDKKGRRAATELLMNNAAIANLIRSEKVHQIQNVMQTSKSSGMHIMS
DSVMKLYEGGIISKEMAFPYSREEIPQ

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=684832 M1J35_RS18340 WP_254653796.1 3573354..3574397(-) (pilT) [Rossellomorea sp. KS-H15a]
TTGAAAGAAAAAGTTGATTATCTTCTTAAATCCGCTTTCGAGCTGGGGGCGTCGGATATCCATTTGACCGTGGGTTCCCC
GCCTATCTTCCGCATACACGGGGATTTAAAAAGATACGGGAAAGAGAACCTGTTGCCCGATCATACAGAGTTAATGGCAA
AAGCCATCATTTCAGAGACAATGTATCCTGCTTTTGAAGAAGTGGGTGAGCTGGATTTTTCCTATGGGATTCCCGGCGTG
TCAAGATTCAGGGTGAATGCATTTAAGCAGCGATCATGTGTATCCTTGGCTATACGTGTGATTCCAACTTCCATCCCAAC
AATGGAAGATCTGTTTCTTCCACATGCATTGAAGGCCATTGCCGAAAAACCTCAGGGACTGTTCCTCGTAACAGGGCCAA
CGGGAAGCGGGAAGTCTACCACACTCGCTTCCATCGTCGATTATATGAACCGGAAGATGAGGAAGCACATCATCACCCTG
GAAGATCCAATCGAGTACCTGCACAAACACGGTGCATCGATCATTGATCAGCGGGAAGTGGGGTTTGATACAAGGTCGTT
CTCAAAAGGACTGCGAAGTGCCCTGAGGCAGGATCCGGATATCATTCTTGTAGGAGAGATGCGTGACTTGGAGACCATCC
ATACGGCCATCACGGCAGCTGAGACCGGTCACCTTGTACTTGCGACCCTACACACATCCAGTGCGCCTGCCACGATCGAG
CGGATCGTAGACGTCTTCCCTCCGGAGCAGCAGTCACAAATCCGCGTTCAGTTAGCCTCGGTCCTTGTCGGGATCCTTTC
CCAAAGGCTATTTGCCACAGTGGATAAAAAGGGGAGAAGGGCAGCGACGGAACTCCTGATGAATAATGCGGCGATCGCCA
ATCTGATCCGTTCTGAAAAGGTCCACCAGATTCAAAATGTCATGCAGACGTCTAAAAGTTCAGGCATGCACATCATGAGC
GACAGTGTGATGAAACTGTATGAAGGTGGCATCATATCAAAGGAAATGGCGTTTCCTTACAGCAGAGAGGAGATTCCTCA
GTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.829

94.524

0.49
  pilT Pseudomonas aeruginosa PAK

48.105

98.847

0.476
  pilT Pseudomonas stutzeri DSM 10701

49.401

96.254

0.476
  pilT Legionella pneumophila strain Lp02

47.447

95.965

0.455
  pilT Legionella pneumophila strain ERS1305867

47.447

95.965

0.455
  pilT Acinetobacter nosocomialis M2

47.273

95.101

0.45
  pilT Acinetobacter baumannii D1279779

47.273

95.101

0.45
  pilT Acinetobacter baumannii strain A118

47.273

95.101

0.45
  pilT Acinetobacter baylyi ADP1

46.97

95.101

0.447
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.646

94.524

0.441
  pilT Vibrio cholerae strain A1552

46.646

94.524

0.441
  pilT Neisseria meningitidis 8013

46.417

92.507

0.429
  pilT Neisseria gonorrhoeae MS11

46.106

92.507

0.427
  pilU Pseudomonas stutzeri DSM 10701

41.617

96.254

0.401
  pilU Vibrio cholerae strain A1552

40.994

92.795

0.38
  pilU Acinetobacter baylyi ADP1

39.571

93.948

0.372